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MitoPediaAlert2020 Archive

From Bioblast
Currently Answered Questions from the »Oroboros Ecosystem Agenda«

May 2020

  • May-07 Th
MitoPedia: SUIT Do you know how to evaluate fatty acid oxidation (FAO, F-pathway) avoiding to overestimate it?
Did you know that malate alone might support mt-respiration in mitochondrial preparations, if malate anaplerotic pathways (e.g. mt-malic enzyme) are present?
- Learn more about - »malate anaplerotic pathway control state«
- DatLab 7.4 provides a step-by-step guide through the SUIT-027 to evaluate the malate anaplerotic pathway - »SUIT-027«
- Since F-pathway can be overestimated due to anaplerotic reactions, SUIT-002 was specially designed to give information on F-pathway in OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways - »SUIT-002«

Apr 2020

  • Apr-24 Fr
Electron transport chain
MitoPedia ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the electron transfer system.

- »Hatefi 1962 J Biol Chem-XLII«



  • Apr-21 Tu
Citrate synthase
MitoPedia Citrate synthase activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.

- »MiPNet17.04 CitrateSynthase«



  • Apr-13 Mo
MitoPedia: SUIT Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases?

- DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the mitochondrial preparation - »SUIT-024«



  • Apr-08 We
MitoPedia: SUIT The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.

- DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum digitonin concentration. - »SUIT-010«


Mar 2020

  • Mar-16 Mo
MitoPedia: SUIT The standardized coupling control protocol to explore the bioenergetics of living cells

- DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. - »SUIT-003«



  • Mar-11 We
MitoPedia Does blebbistatin reduce oxygen dependence of respiration of permeabilized muscle fibers? This is one of the questions addressed in the COST Action CA15203 MitoEAGLE WG2.

- »Blebbistatin«



  • Mar-06 Fr
MitoPedia: SUIT Explore metabolic profiles and specific modulations of mitochondrial pathways in health and disease with SUIT reference protocols.

- »RP1« and »RP2«



  • Mar-03 Tu
MitoPedia Rapamycin - more differences or more in common with other mitObesity drugs?

- »Rapamycin«


Feb 2020

  • Feb-26 We
MitoPedia It is essential to titrate uncouplers in multiple steps for respirometric measurement of electron transfer capacity.

- »Noncoupled respiration«



  • Feb-24 Mo
MitoPedia More research is needed on the impact of viral infection on mitochondrial function.

- »Viruses and mitochondrial medicine«



  • Feb-19 We
MitoPedia Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function.

- »Spermidine«



  • Feb-18 Tu
MitoPedia Why do we fight gas bubbles?

- Dive deep into »Oxygen solubility«



  • Feb-12 We
MitoPedia What do metformin, melatonin and other bioactive mitObesity compounds have in common?

- »MitObesity drugs«


Jan 2020

  • Jan-16 Th
MitoPedia Oxygen flux

- which SI units should be used?


MitoPedia Did you ever think that units for body mass could be an issue?

- »Body mass«



  • Jan-08 We
MitoPedia Force and Energy: this we all need in the New Year - and MitoPedia may help to clarify the difference.