Difference between revisions of "Template:SUIT low ADP"
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In the presence of endogenous substrates, the addition of a low concentration of [[ADP]] (0.1 ฮผM) is a crucial step to detect the fully | In the presence of endogenous substrates, the addition of a low concentration of [[ADP]] (0.1 ฮผM) is a crucial step to detect the fully oxidized [[NAD]] for the calculation of the [[NAD]] redox state. ADP stimulates the consumption of endogenous substrates, which can be observed as a decrease in respiration and the NADH fluorescence signal. |
Revision as of 13:40, 21 December 2023
In the presence of endogenous substrates, the addition of a low concentration of ADP (0.1 ฮผM) is a crucial step to detect the fully oxidized NAD for the calculation of the NAD redox state. ADP stimulates the consumption of endogenous substrates, which can be observed as a decrease in respiration and the NADH fluorescence signal.