Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Template talk:Next oroboros ecosystem agenda"

From Bioblast
Line 28: Line 28:


=== Next ===
=== Next ===
{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| '''Did you know that if [[ET_capacity|ET capacity]] is lower than [[OXPHOS_capacity|OXPHOS capacity]] (''P''>''E'') in living cells, or in mitochondrial preparations, then you face an experimental artefact? The reasons which may trigger this experimental artefact are:''' <br>
- loss of oxidative capacity during the time course of the respirometric assay,<br>
- insufficient uncoupler concentrations,<br>
- over titration of uncoupler, which inhibits ET capacity,<br>
- high concentration of the [https://www.bioblast.at/index.php/Oligomycin oligomycin] (inhibitor of the phosphorylation pathway) used to measure [https://wiki.oroboros.at/index.php/LEAK_respiration LEAK respiration] prior ET capacity.
|}


== O2k-Brief alert ==
== O2k-Brief alert ==

Revision as of 10:24, 15 June 2020

Antunes Diana (talk) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:
 {|
 | logos
 | title in bold <br>
 - link
 |}
 

e.g.,

O2k-Open SupportWarning: loss of data Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?

- »Instrumental background« and »TIP2k Filter Papers«

See the scheme:


Agenda/Alerts body text format


Bioblast alert 2020

Prep for circular done here: Talk:Bioblast alert 2020

MitoPedia alert

Next

MitoPedia
Did you know that if ET capacity is lower than OXPHOS capacity (P>E) in living cells, or in mitochondrial preparations, then you face an experimental artefact? The reasons which may trigger this experimental artefact are:

- loss of oxidative capacity during the time course of the respirometric assay,
- insufficient uncoupler concentrations,
- over titration of uncoupler, which inhibits ET capacity,
- high concentration of the oligomycin (inhibitor of the phosphorylation pathway) used to measure LEAK respiration prior ET capacity.

O2k-Brief alert

Next

O2k-Publications alert

Next

O2k-Open Support alert

Next

  • While in Home Office: Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.



Removed for correction

O2k-Open SupportWarning: loss of data Do you perform the leaky chamber test after chamber assembly?

An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly.
- »Leaky chamber test« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«

O2k-Open SupportWarning: loss of data Find out how to evaluate the effect of the obtained ‘O2 slope neg’ noise on experimental results

- » QC1: Oxygen sensor test«

O2k-Open Support alert circulars

O2k-Open Support alert circular 2020(01): in prep

New O2k-Open SupportWarning: loss of data A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.

See how you can check that the two blue OroboPOS-Holders are intact.
- »O2k-OroboPOS-Holder«

New O2k-Open SupportTake care Calibration parameters are transferred to experimental DatLab files

Save calibration information from selected Marks while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files.
- » Oxygen calibration - DatLab«

O2k-Open SupportWarning: loss of data Do you perform O2k-Chamber volume calibration?

The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module).
- »O2k-Chamber MitoPedia«

O2k-Open SupportTake care Do you use the correct O2 solubility factor (FM) in the ‘O2 calibration’ window in DatLab?

The O2 solubility factor varies according to the medium used and influences the air calibration.
- »Oxygen solubility factor«

In preparation

For discussion

Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 (if the specific alert section is yet to be attributed)
MitoPedia: SUIT Controlled stability of the MitoKit-CII based on HPLC and LCMS analysis: No degradation of MitoKit-CII compounds MitoKit-CII/Succinate-nv (NV118) and MitoKit-CII/Malonate-nv (NV161) for following storage conditions:
  • Proven -80 °C solid stability for more than 3 years (3 years 7 months)
  • Proven -20 °C solid stability for 1 year
  • Proven DMSO stability at room temperature for 1 week
  • Proven DMSO stability at -20 °C for 6 months (ongoing)

2021

Postponed events

FAT4BRAIN » FAT4BRAIN School Riga LV IOC147«
»APS2020 Chicago US«
O2k-Workshops San Diego CA US