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Difference between revisions of "Template talk:Next oroboros ecosystem agenda"

From Bioblast
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- communicated by »[[Doerrier Carolina]]«
- communicated by »[[Doerrier Carolina]]«
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[[User:Cecatto Cristiane|Cecatto Cristiane]] ([[User talk:Cecatto Cristiane|talk]]) 17:08, 08 October 2020 (CEST): What do you think about including the section of the MiPNet where is written the answer? The question is how or where? And what about an internal link? But then it doesn't open directly the pdf: [[MiPNet19.03 O2k-cleaning and ISS]]
 
[[User:Doerrier Carolina|Doerrier Carolina]] ([[User talk:Doerrier Carolina|talk]]) 15:17, 19 October 2020 (CEST) Yes, this is the idea, but we had to finish the updates in the MiPNet.
::: [[User:Cecatto Cristiane|Cecatto Cristiane]] ([[User talk:Cecatto Cristiane|talk]]) 17:08, 08 October 2020 (CEST): What do you think about including the section of the MiPNet where is written the answer? The question is how or where? And what about an internal link? But then it doesn't open directly the pdf: [[MiPNet19.03 O2k-cleaning and ISS]]
::: [[User:Doerrier Carolina|Doerrier Carolina]] ([[User talk:Doerrier Carolina|talk]]) 15:17, 19 October 2020 (CEST) Yes, this is the idea, but we had to finish the updates in the MiPNet.


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Revision as of 14:17, 19 October 2020

Antunes Diana (talk) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:
 {|
 | logos
 | title in bold <br>
 - link
 |}
 

e.g.,

O2k-Open SupportWarning: loss of data Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?

- »Instrumental background« and »TIP2k Filter Papers«

See the scheme:


Agenda/Alerts body text format

Bioblast alert 2020

Prep for circular done here: Talk:Bioblast alert 2020

MitoPedia alert

Next

Oct-29

MitoPedia
Would you like to monitor O2 flux production/consumption in response to light?

Check all the news here about the PB-Module, the new module in the NextGen-O2k that will allow to conduct experiments in PhotoBiology!
- »PB-Module«, »NextGen-O2k«, »PhotoBiology« 
- communicated by »Huete-Ortega M« 

  • xxx
MitoPedia
How can you measure mitochondrial membrane potential with O2k-Fluorometry?

Learn more about how to measure mt-membrane potential in mitochondrial preparations with safranin, TMRM or Rhodamine 123.
- »Safranin«, »TMRM«, »Rhodamine 123« 
- communicated by »Komlodi Timea« and »Iglesias-Gonzalez Javier« 

or

MitoPedia
How to measure mitochondrial membrane potential with O2k-Fluorometry?

Learn more about how to measure mt-membrane potential in mitochondrial preparations with safranin, TMRM or Rhodamine 123.
- »Safranin«, »TMRM«, »Rhodamine 123« 
- communicated by »Komlodi Timea« and »Iglesias-Gonzalez Javier« 

  • Oct-?? ??
MitoPedia
Human PBMC and Platelet as non-invasive samples for HRR

Two isolations procedures described in MiPNet21.17 Blood Cells Isolation.
- » (we need something here) « 
- communicated by »Cecatto Cristiane« and »[[]]« 

O2k-Brief alert

Next

O2k-Publications alert

Author names of O2k-Publications/ Brief for the Agenda vs Bioblast

1.Oroboros Ecosystem Agenda: SOP: Surname with long version and First name with short version. Please, see: Template alerts.
Example: Oroboros Ecosystem Agenda example
This means that we have to edit manually the author names within the O2k-Publications/ Brief alerts (i.e., Antunes Diana (from Bioblast) to Antunes D (to Agenda entries)) if they are long or copy from the gray box.
2.Bioblast: SOP: [1] – examples are available using both Surname and First name with long version (e.g., Antunes Diana). If in Bioblast the authors are with short surname in the links please update it to complete surname, guidelines are below.
Click to expand or collaps

Guidelines to update a reference

When creating an O2k-Publication/Brief alert, it is the responsibility of the author to check if the reference is accordingly to the correct guidelines:

Reference example.png

Correct format for a person called “First Name Surname”:
In the gray box should be “Surname FN” - From where references to be used as O2k-Publication/Brief alerts are copied from.

Gray box reference.png

In the links should be “ Surname First N”

Authors names links reference.png

How to edit:
A) Check if all authors with profiles in Bioblast (blue links) have their profiles with full name. If they have, proceed to next step (B). If not, you need to move it following the steps “How to move a profile” below.
B) Click in “Edit with form”.
C) The gray box is edited in “Reference” and the links are changed in “Author(s)”. Change it accordingly and click in “save page” at the end of the page.

Editing reference.png

How to move a profile (instructions sent by Marija):

So let’s take for example my page: Beno M [2]

As you can see there is a redirect from Beno Marija to Beno M. What has to be done is to switch the content of those pages. Here are the steps:
1. Go to e.g. “Beno M” on edit
2. Click on “Move”
3. Change the new title to the full name of the person. In my case it will be Beno M to Beno Marija

BenoMreference.jpg

4. Now it should look like that:

BenoMarijaReference.png

5. Click on “Move” and leave all the ticked boxes
6.Done
After that you can continue in step B.
If you have any doubt you can contact Cristiane, Marija or Mario.


Next

---

O2k-Open Support alert

  • While in Home Office: Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.

Next

O2k-Open SupportInfo: improve What is the advantage of using flux control ratios (FCRs)?


FCRs provide information on coupling and substrate control of mitochondrial respiration independent of (i) mt-content in cells or tissues, (ii) purification of isolated mitochondrial preparations, and (iii) assay conditions for determination of tissue mass or mt-markers.
- »Flux control ratio«


O2k-Open SupportInfo: improve Do you know how to insert properly the ISS-tip into the O2k-chamber?


- »MiPNet19.03 O2k-cleaning and ISS« 
- communicated by »Doerrier Carolina«

Cecatto Cristiane (talk) 17:08, 08 October 2020 (CEST): What do you think about including the section of the MiPNet where is written the answer? The question is how or where? And what about an internal link? But then it doesn't open directly the pdf: MiPNet19.03 O2k-cleaning and ISS
Doerrier Carolina (talk) 15:17, 19 October 2020 (CEST) Yes, this is the idea, but we had to finish the updates in the MiPNet.
O2k-Open SupportInfo: improve Do you know how to prepare a permeabilized muscle fibers?


- »O2k-Videosupport: Fiber permeabilization« 
- »Doerrier 2018 Methods Mol Biol« 
- communicated by »Doerrier Carolina«

  • xxxx
O2k-Open SupportInfo: improve Have you observed a high or unstable zero current?

Cleaning and drying the OroboPOS-Connector frequently solves this problem.
- »Cleaning the OroboPOS-Connector« 
- communicated by »Schmitt Sabine«

O2k-Open SupportInfo: improve Have you observed a high or unstable zero current?

A high or unstable zero current might be caused by the O2k-Main Unit, by the OroboPOS-Connector, by the OroboPOS or by bad connection between these components. High zero current test helps you to localize the problem.
- Visit our website to find out how to perform high zero current test: »High zero current test« 
- communicated by »Schmitt Sabine« and »Komlodi Timea«

Removed for correction

O2k-Open SupportWarning: loss of data Do you perform the leaky chamber test after chamber assembly?

An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly.
- »Leaky chamber test« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«

O2k-Open Support alert circulars

In preparation

For discussion

Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 (if the specific alert section is yet to be attributed)
MitoPedia: SUIT Controlled stability of the MitoKit-CII based on HPLC and LCMS analysis: No degradation of MitoKit-CII compounds MitoKit-CII/Succinate-nv (NV118) and MitoKit-CII/Malonate-nv (NV161) for following storage conditions:
  • Proven -80 °C solid stability for more than 3 years (3 years 7 months)
  • Proven -20 °C solid stability for 1 year
  • Proven DMSO stability at room temperature for 1 week
  • Proven DMSO stability at -20 °C for 6 months (ongoing)

2021

Postponed events

O2k-Workshops Pre-Conference O2k-Workshop »MiPNet25.11 IOC148 Riga LV « March 2021?
EU-CARDIOPROTECTION »6th EU-Cardioprotection Meeting 2020 Riga LV« (Oct 7-9)- March 2021?
»APS2020 Chicago US«
O2k-Workshops San Diego CA US
Bioenergetics Vienna 2020.jpg Bioenergetics Vienna 2020
O2k-Workshops IOC Schroecken AT
»36th Congress Czech Nutrition Society 2020 Hradec Kralove CZ« (Sep 10-12)