User talk:Spitzer Gudrun
From Bioblast
Asc gutest 1
Application in HRR
- As: Ascorbate sodium salt (Sodium L-ascorbate); Sigma A 7631, 100 g, store at RT; FW = 198.1.
- Caution: Light sensitive (store solution in a dark glass vial).
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- Preparation of 800 mM stock solution (dissolved in H2O) for use in 2-mL O2k-chamber:
- Weigh 1.584 g of ascorbate sodium salt and dissolve in 10 mL H2O
- To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL-1 solution of pH 2).
- Divide into 0.2 mL portions.
- Store frozen at -20 Β°C.
- Preparation of 1000 mM stock solution (dissolved in H2O) for use in 0.5-mL O2k-chamber:
- Weigh 1.980 g of ascorbate sodium salt and dissolve in 10 mL H2O
- To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL-1 solution of pH 2).
- Divide into 0.2 mL portions.
- Store frozen at -20 Β°C.
- Β»O2k manual titrations: MiPNet09.12
- Titration volume (2-mL O2k-chamber): 5 Β΅L 800mM using a 25 Β΅L Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 1 Β΅L 1000mM using a 10 Β΅L Hamilton syringe
- Final concentration: 2 mM.
Asc gutest 2
Application in HRR
- As: Ascorbate sodium salt (Sodium L-ascorbate); Sigma A 7631, 100 g, store at RT; FW = 198.1.
- Caution: Light sensitive (store solution in a dark glass vial).
- Preparation of 800 mM stock solution (dissolved in H2O) for 2-mL O2k-chamber:
- Weigh 1.584 g of ascorbate sodium salt and dissolve in 10 mL H2O
- To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL-1 solution of pH 2).
- Divide into 0.2 mL portions.
- Store frozen at -20 Β°C.
- Β»O2k manual titrations: MiPNet09.12
- Titration volume (2-mL O2k-chamber): 5 Β΅L 800 mM using a 25 Β΅L Hamilton syringe.
- Final concentration: 2 mM.
- Preparation of 1000 mM stock solution (dissolved in H2O) for 0.5-mL O2k-chamber:
- Weigh 1.980 g of ascorbate sodium salt and dissolve in 10 mL H2O
- To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL-1 solution of pH 2).
- Divide into 0.2 mL portions.
- Store frozen at -20 Β°C.
- Β»O2k manual titrations: MiPNet09.12
- Titration volume (0.5-mL O2k-chamber): 1 Β΅L 1000 mM using a 10 Β΅L Hamilton syringe.
- Final concentration: 2 mM.