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Difference between revisions of "User talk:Zhang Feiyuan/My sandbox 3"

From Bioblast
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The Clark Oxygen Sensor
{{Product
|description='''Stirrer-Bar sV\white [[PVDF]]\11.5x6.2 mm''', operated in the [[O2k-sV-Chamber]] at constant stirring speed (standard is 750 rpm, or 12.5 Hz), to provide optimum mixing of the sample in the aqueous medium and ensure a stable signal of the polarographic oxygen sensor ([[OroboPOS]]) placed in a position of maximum current of the medium.


[http://chem.ch.huji.ac.il/ Dr. Leland Clark] - inventor of the "Clark Oxygen Sensor" (1954); the Clark type polarographic oxygen sensor remains the gold standard for measuring dissolved oxygen in biomedical, environmental and industrial applicationsย  - Obituary
Two units of this item are standard components of the [[O2k-sV-Chamber]].
<font color="#008B8B">(link,doesnt work) </font>;
[http://www.ncbi.nlm.nih.gov/pubmed/3519875?dopt=Abstract Review]


|product id=23421-01
|product type=O2k, O2k-sV-Chamber
|info
|product image=TO REPLACE [[Image:Stirrer bar PVDF.jpg|right|180px]]
}}


Convenience and simplicity
CHECK THE TEXT BELOW


* For dispatch of the [[O2k-sV-Chamber]], the Stirrer-Bars sV are shipped in the [[Storage box sV]].


'The convenience and simplicity of the polarographic 'oxygen electrode' technicque for measuring rapid changes in the rate of oxygen utilization by cellular and subcellular systems is now leading to its more general application in many laboratories. The types and design of oxygen electrodes vary, depending on the invetigator's ingenuity and specific requirements of the system under investigation.'
A "jumping" stirring bar problem is frequently caused by small particles sticking to the glass chamber, not the stirring bar itself. >> [[MiPNet19.03 O2k-cleaning and ISS]].
ย 
Estabrook R (1967) Mitochondrial respiratory control and the polarographic measurement of ADP:O ratios. Methods Enzymol. 10: 41-47.
ย 
ย 
More precise information
ย 
"However, one approach that is underutilized in whole-cell bioenergetics, and that is accessible as long as cells can be obtained in suspension, is the oxygen electrode, which can obtain more precise information on the bioenergetic status of the in situ mitochondria than more โ€˜high-techโ€™ approaches such as fluorescent monitoring of ฮ”ฯˆm.โ€ย 
ย 
Nicholls DG, Ferguson S (2002) Bioenergetics 3. Academic Press, London.

Revision as of 16:11, 17 June 2019

                



User talk:Zhang Feiyuan/My sandbox 3

O2k-Catalogue

Description Stirrer-Bar sV\white PVDF\11.5x6.2 mm, operated in the O2k-sV-Chamber at constant stirring speed (standard is 750 rpm, or 12.5 Hz), to provide optimum mixing of the sample in the aqueous medium and ensure a stable signal of the polarographic oxygen sensor (OroboPOS) placed in a position of maximum current of the medium.

Two units of this item are standard components of the O2k-sV-Chamber.

Product ID 23421-01
Type O2k, O2k-sV-Chamber
Link
Image TO REPLACE
Stirrer bar PVDF.jpg


CHECK THE TEXT BELOW

A "jumping" stirring bar problem is frequently caused by small particles sticking to the glass chamber, not the stirring bar itself. >> MiPNet19.03 O2k-cleaning and ISS.