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Difference between revisions of "Ye 2011 J Biol Chem"

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{{Publication
{{Publication
|title=Ye F, Lemieux H, Hoppel CL, Hanson RW, Hakimi P, Croniger CM, Puchowicz M, Anderson VE, Fujioka H, Stavnezer E (2011) PPAR{gamma} MediatesĀ  a Ski-induced Shift from Glycolysis to OxidativeĀ  Energy Metabolism. J. Biol. Chem.Ā  2011 Sep 29. [Epub ahead of print].
|title=Ye F, Lemieux H, Hoppel CL, Hanson RW, Hakimi P, Croniger CM, Puchowicz M, Anderson VE, Fujioka H, Stavnezer E (2011) Peroxisome proliferator-activated receptor Ī³ (PPARĪ³) mediates a Ski-induced shift from glycolysis to oxidative energy metabolism. J Biol Chem 286:40013-24.
|info=[http://www.ncbi.nlm.nih.gov/pubmed/21917928 PMID:21917928 ]
|info=[http://www.ncbi.nlm.nih.gov/pubmed/21917928 PMID:21917928 Open Access]
|authors=Ye F, Lemieux H, Hoppel CL, Hanson RW, Hakimi P, Croniger CM, Puchowicz M, Anderson VE, Fujioka H, Stavnezer E
|authors=Ye F, Lemieux H, Hoppel CL, Hanson RW, Hakimi P, Croniger CM, Puchowicz M, Anderson VE, Fujioka H, Stavnezer E
|year=2011
|year=2011
|journal=J. Biol. Chem.
|journal=J Biol Chem
|abstract=Over-expression of the Ski oncogene induces oncogenic transformation of chicken embryo fibroblasts (CEFs). However, unlike most other oncogene-transformed cells, Ski-transformed CEFs (Ski-CEFs) do not display the classical Warburg effect. On the contrary, Ski transformation reduced lactate production and glucose utilization in CEFs. Compared to CEFs, Ski-CEFs exhibited enhanced TCA cycle activity, fatty acid catabolism through Ī²-oxidation, glutamate oxidation, oxygen consumption, as well as, increased numbers and mass of mitochondria. Interestingly, expression of PPARĪ³, a key transcription factor that regulates adipogenesis and lipid metabolism was dramatically elevated at both the mRNA and protein levels in Ski-CEFs. Accordingly, PPARĪ³ target genes that are involved in lipid uptake, transport and oxidation were also markedly up-regulated by Ski. Knocking down PPARĪ³ in Ski-CEFs by RNA interference reversed the elevated expression of these PPARĪ³ target genes, as well as the shift to oxidative metabolism and the increased mitochondrial biogenesis. Moreover, we found that Ski co-immunoprecipitates with PPARĪ³ and co-activates PPARĪ³-driven transcription.
|abstract=Over-expression of the Ski oncogene induces oncogenic transformation of chicken embryo fibroblasts (CEFs). However, unlike most other oncogene-transformed cells, Ski-transformed CEFs (Ski-CEFs) do not display the classical [[Warburg effect]]. On the contrary, Ski transformation reduced lactate production and glucose utilization in CEFs. Compared to CEFs, Ski-CEFs exhibited enhanced TCA cycle activity, fatty acid catabolism through Ī²-oxidation, glutamate oxidation, oxygen consumption, as well as, increased numbers and mass of mitochondria. Interestingly, expression of PPARĪ³, a key transcription factor that regulates adipogenesis and lipid metabolism was dramatically elevated at both the mRNA and protein levels in Ski-CEFs. Accordingly, PPARĪ³ target genes that are involved in lipid uptake, transport and oxidation were also markedly up-regulated by Ski. Knocking down PPARĪ³ in Ski-CEFs by RNA interference reversed the elevated expression of these PPARĪ³ target genes, as well as the shift to oxidative metabolism and the increased mitochondrial biogenesis. Moreover, we found that Ski co-immunoprecipitates with PPARĪ³ and co-activates PPARĪ³-driven transcription.
|keywords=Chicken embryo fibroblasts (CEFs),
|mipnetlab=US_OH_Cleveland_Hoppel_CL, CA_Edmonton_Lemieux_H
|mipnetlab=US_OH_Cleveland_Hoppel_CL, CA_Edmonton_Lemieux_H
}}
}}
{{Labeling
{{Labeling
|area=Respiration, mt-Biogenesis;mt-density
|organism=Chicken
|tissues=Fibroblast
|preparations=Intact cells
|topics=Aerobic glycolysis
|couplingstates=ROUTINE
|instruments=Oxygraph-2k
|instruments=Oxygraph-2k
|organism=Other Non-Mammal
|tissues=Fibroblast
|preparations=Intact Cell; Cultured; Primary, Permeabilized Cell or Tissue; Homogenate
|topics=Respiration; OXPHOS; ETS Capacity
}}
}}

Latest revision as of 14:47, 9 November 2016

Publications in the MiPMap
Ye F, Lemieux H, Hoppel CL, Hanson RW, Hakimi P, Croniger CM, Puchowicz M, Anderson VE, Fujioka H, Stavnezer E (2011) Peroxisome proliferator-activated receptor Ī³ (PPARĪ³) mediates a Ski-induced shift from glycolysis to oxidative energy metabolism. J Biol Chem 286:40013-24.

Ā» PMID:21917928 Open Access

Ye F, Lemieux H, Hoppel CL, Hanson RW, Hakimi P, Croniger CM, Puchowicz M, Anderson VE, Fujioka H, Stavnezer E (2011) J Biol Chem

Abstract: Over-expression of the Ski oncogene induces oncogenic transformation of chicken embryo fibroblasts (CEFs). However, unlike most other oncogene-transformed cells, Ski-transformed CEFs (Ski-CEFs) do not display the classical Warburg effect. On the contrary, Ski transformation reduced lactate production and glucose utilization in CEFs. Compared to CEFs, Ski-CEFs exhibited enhanced TCA cycle activity, fatty acid catabolism through Ī²-oxidation, glutamate oxidation, oxygen consumption, as well as, increased numbers and mass of mitochondria. Interestingly, expression of PPARĪ³, a key transcription factor that regulates adipogenesis and lipid metabolism was dramatically elevated at both the mRNA and protein levels in Ski-CEFs. Accordingly, PPARĪ³ target genes that are involved in lipid uptake, transport and oxidation were also markedly up-regulated by Ski. Knocking down PPARĪ³ in Ski-CEFs by RNA interference reversed the elevated expression of these PPARĪ³ target genes, as well as the shift to oxidative metabolism and the increased mitochondrial biogenesis. Moreover, we found that Ski co-immunoprecipitates with PPARĪ³ and co-activates PPARĪ³-driven transcription. ā€¢ Keywords: Chicken embryo fibroblasts (CEFs)

ā€¢ O2k-Network Lab: US_OH_Cleveland_Hoppel_CL, CA_Edmonton_Lemieux_H


Labels: MiParea: Respiration, mt-Biogenesis;mt-density 


Organism: Chicken  Tissue;cell: Fibroblast  Preparation: Intact cells 

Regulation: Aerobic glycolysis  Coupling state: ROUTINE 

HRR: Oxygraph-2k