News and Events | Working Groups | Short-Term Scientific Missions | Management Committee | Members |
COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE preprint 2017-09-21
MitoEAGLE preprint 2018-02-18(21) The protonmotive force and respiratory control. - http://www.mitoeagle.org/index.php/MitoEAGLE_preprint_2017-09-21. |
Β» - Β» Versions -- Next step Β» MitoEAGLE preprint 2018-02-08
MitoEAGLE (2018) MitoEAGLE preprint
Abstract: Note: Subscript βΒ§β indicates throughout the text those parts, where potential differences provide a mathematically correct but physicochemically incomplete description and should be replaced by stoichiometric potential differences (Gnaiger 1993b). A unified concept on vectorial motive transformations and scalar chemical reactions will be derived elsewhere (Gnaiger, in prep.). Appreciation of the fundamental distinction between differences of potential versus differences of stoichiometric potential may be considered a key to critically evaluate the arguments presented in Section 3 on the protonmotive force. Since this discussion appears to be presently beyond the scope of a MitoEAGLE position statement, Section 3 will be removed from the next version and final manuscript. This section should become a topic of discussion within Working Group 1 of the MitoEAGLE consortium, following a primary peer-reviewed publication of the concept of stoichiometric potential differences. β’ Keywords: Mitochondrial respiratory control, coupling control, mitochondrial preparations, protonmotive force, chemiosmotic theory, oxidative phosphorylation, OXPHOS, efficiency, electron transfer, ET; proton leak, LEAK, residual oxygen consumption, ROX, State 2, State 3, State 4, normalization, flow, flux
- Citation:
- MitoEAGLE preprint 2017-09-21(Version 21). The protonmotive force and respiratory control. - http://www.mitoeagle.org/index.php/MitoEAGLE_preprint_2017-09-21
Authors
- Contributing co-authors: Confirming to have read the final manuscript, possibly to have made additions or suggestions for improvement, and to agree to implement the recommendations into future manuscripts, presentations and teaching materials. (alphabetical, to be extended)
- Supporting co-authors: Confirming to have read the final manuscript, and to agree to implement the recommendations into future manuscripts, presentations and teaching materials. (alphabetical, to be extended)
- Building blocks of mitochondrial physiology MitoEAGLE recommendations Part 1.
- Phase 3: Β» MitoEAGLE preprint 2018-02-08
- Phase 2: Β» MitoEAGLE preprint 2017-09-21
Section 2: Oxidative phosphorylation and coupling states in mitochondrial preparations
Section 3: The protonmotive force and proton flux
Section 4: Normalization: fluxes and flows
Labels: MiParea: Respiration, mt-Awareness
Preparation: Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria
Enzyme: Marker enzyme
Regulation: Coupling efficiency;uncoupling, Flux control, mt-Membrane potential, Uncoupler
Coupling state: LEAK, OXPHOS, ET
Pathway: ROX