SUIT-002 O2 mt D005: Difference between revisions
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| SUIT reference protocol RP2 for permeabilized cells (pce) with digitonin | | SUIT reference protocol RP2 for permeabilized cells (pce) with digitonin | ||
| style="text-align:center"| [[File:Download1.png|20px|link=http:// | | style="text-align:center"| [[File:Download1.png|20px|link=http://www.bioblast.at/images/1/14/RP2pce%28Dig%29.DLP]] | ||
| style="text-align:center"| [[File:Download2.png|20px|link=http://wiki.oroboros.at/images/c/ca/RP2pce%28Dig%29.xlsx]] | | style="text-align:center"| [[File:Download2.png|20px|link=http://wiki.oroboros.at/images/c/ca/RP2pce%28Dig%29.xlsx]] | ||
Revision as of 11:57, 23 October 2017
Description
Abbreviation: FNSGp(PGM)
Reference: A: SUIT_RP2 - MiPNet21.06 SUIT RP
MitoPedia concepts:
SUIT protocol,
SUIT A
- MitoPedia: SUIT - SUIT reference protocol RP2
- SUIT-category: FNSGp(PGM)
- SUIT protocol pattern: diametral
Harmonized SUIT protocols
- SUIT RP1: 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd
- SUIT RP2: 1D;1M.1;2Oct;2c;2M2;3P;4G;5S;6Gp;7U;8Rot;9Ama;10Tm;11Azd
Harmonization between RP1 and RP2
RP2 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see Digitonin). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet pathway control states.
- SUIT RP1 in cells: ce1;1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd
- SUIT RP2 in cells: ce1;1Dig;1D;1M.1;2Oct;2c;2M2;3P;4G;5S;6Gp;7U;8Rot;9Ama;10Tm;11Azd
File:RP1 RP2 harmonization cells.jpg Harmonization between RP1 and RP2 in cells
SUIT O2 DLP for RP2 (DatLab 7.1)
- DL-Protocols (DLP) for substrate-uncoupler-inhibitor titrations (see MitoPedia: SUIT) guide through a sequence of coupling and pathway control states. A specific SUIT protocol can be assigned to O2k-Chamber A or B or both. DL-Protocols for different SUIT protocols can be found in the library of protocols MitoPedia: SUIT. Instrumental DL-protocols are displayed in the DL-Protocol library.
Download instructions.
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