Difference between revisions of "ADP"

Revision as of 11:07, 10 November 2010

high-resolution terminology - matching measurements at high-resolution

ADP

Description

Adenosine 5’diphosphate (D), C10H15N5O10P2K, potassium salt; substrate of ANT and ATP synthase.

Abbreviation: D

Reference: MiPNet03.02, MiPNet09.12,

Labels:

Regulation: ATP; ADP; AMP; PCr"ATP; ADP; AMP; PCr" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.

HRR

D: ADP (Sigma,A 5285: Adenosine 5’diphosphate, C₁₀H₁₅N5O10P2K, potassium salt, MW=491.2)

Preparation of stock solution (dissolved in H2O):

# 491.2 mg/2 ml of final volume. Weigh 491.2 mg of ADP, add 1 ml H2O and 450 ul 5 N KOH. ADP will dissolve after addition of KOH. Check pH and adjust to 7 (solution A).

# 500 mM ADP stock solution: Adjust the total volume of solution A to 2 ml and divide into 0.2 ml portions. Store frozen at -80 °C.

# 500 mM ADP stock solution with 3 mM free Mg2+: To keep free [Mg2+] constant at 3 mM when titrating the ADP stock solution into MiR06, mix ADP (solution A) with MgCl2 (0.6 mol/mol ADP). Adjusting pH of ADP to 7, add 121.98 mg of MgCl2 (Scharlau MA0036: MgCl2.6H2O, MW=203.3). White precipitate will occurr, which will be dissolved after 1-2 min of stirring on magnetic stirrer at room temperature. Adjust final volume to 2 ml, divide into 0.2 ml portions and store at -80 °C.

Final conc. in 2 ml O2k-chamber: 2-5 mM; titrate 8-20 µl using the 25 µl syringe. <a href="MiPNet09.12">MiPNet09.12</a>

@@ Line 10: / Line 10: @@
 |type=Substrate PS
 }}
-==HRR==
+<h2>HRR</h2>
+<p>D: ADP (Sigma,A 5285: Adenosine 5&rsquo;diphosphate, C<sub>10</sub>H<sub>15</sub>N5O10P2K, potassium salt, MW=491.2)</p>
-D: ADP (Sigma,A 5285: Adenosine 5’diphosphate, C10H15N5O10P2K, potassium salt, MW=491.2)
+<p><strong>Preparation of stock solution</strong> (dissolved in H2O): </p>
+<p># 491.2 mg/2 ml of final volume. Weigh 491.2 mg of ADP, add 1 ml H2O and 450 ul 5 N KOH. ADP will dissolve after addition of KOH. Check pH and adjust to 7 (solution A).</p>
-Preparation of 500 mM (H2O) stock solution: 491.2 mg/2 ml of final volume. Weigh 491.2 mg of ADP, add 1 ml H2O and 450 ul 5 N KOH. ADP will dissolve after addition of KOH. Check pH and adjust to 7, adjust total volume to 2 ml and divide into 0.2 ml portions. Store frozen at -80 °C.
+<p># <strong>500 mM ADP stock solution</strong>: Adjust the total volume of solution A&nbsp;to 2 ml and divide into 0.2 ml portions. Store frozen at -80 &deg;C.</p>
+<p># <strong>500 mM </strong><strong>ADP stock solution with 3 mM free Mg2+</strong>: To keep free [Mg2+] constant at 3 mM when titrating the ADP stock solution into MiR06, mix ADP (solution A)&nbsp;with MgCl2 (0.6 mol/mol ADP). Adjusting pH of ADP to 7, add 121.98 mg of MgCl2 (Scharlau MA0036: MgCl2.6H2O, MW=203.3). White precipitate will occurr, which will be dissolved after 1-2 min of stirring on magnetic stirrer at room temperature. Adjust final volume to 2 ml, divide into 0.2 ml portions and store at -80 &deg;C.</p>
-To keep [Mg2+] constant during respiration measurement mix ADP with MgCl2 (0.6 mol/mol ADP).
+<p>Final conc. in 2 ml O2k-chamber: 2-5 mM; titrate 8-20 &micro;l using the 25 &micro;l syringe. <a href="MiPNet09.12">MiPNet09.12</a></p>
-Preparation of Mg-ADP: After adjusting pH of ADP to 7, add 121.98 mg of MgCl2 (Scharlau MA0036: MgCl2.6H2O, MW=203.3). White precipitate will occurr, which will be dissolved after 1-2 min of stirring on magnetic stirrer. Adjust final volume to 2 ml, divide into 0.2 ml portions and store at -80 °C.
-Final conc. in 2 ml O2k-chamber: 2-5 mM; titrate 8-20 µl using the 25 µl syringe. [[MiPNet09.12]]