Difference between revisions of "ADP"
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|abbr=D | |abbr=D | ||
|description=Adenosine 5’diphosphate (D), C10H15N5O10P2K, potassium salt; substrate of [[ANT]] and [[ATP synthase]]. | |description=Adenosine 5’diphosphate (D), C10H15N5O10P2K, potassium salt; substrate of [[ANT]] and [[ATP synthase]]. | ||
|info=[[MiPNet03.02]], [[MiPNet09.12]] | |info=[[MiPNet03.02]], [[MiPNet09.12]] | ||
|type=Substrate PS | |type=Substrate PS | ||
}} | }} | ||
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<p># <strong>500 mM ADP stock solution</strong>: Adjust the total volume of solution A to 2 ml and divide into 0.2 ml portions. Store frozen at -80 °C.</p> | <p># <strong>500 mM ADP stock solution</strong>: Adjust the total volume of solution A to 2 ml and divide into 0.2 ml portions. Store frozen at -80 °C.</p> | ||
<p># <strong>500 mM </strong><strong>ADP stock solution with 3 mM free Mg2+</strong>: To keep free [Mg2+] constant at 3 mM when titrating the ADP stock solution into MiR06, mix ADP (solution A) with MgCl2 (0.6 mol/mol ADP). Adjusting pH of ADP to 7, add 121.98 mg of MgCl2 (Scharlau MA0036: MgCl2.6H2O, MW=203.3). White precipitate will occurr, which will be dissolved after 1-2 min of stirring on magnetic stirrer at room temperature. Adjust final volume to 2 ml, divide into 0.2 ml portions and store at -80 °C.</p> | <p># <strong>500 mM </strong><strong>ADP stock solution with 3 mM free Mg2+</strong>: To keep free [Mg2+] constant at 3 mM when titrating the ADP stock solution into MiR06, mix ADP (solution A) with MgCl2 (0.6 mol/mol ADP). Adjusting pH of ADP to 7, add 121.98 mg of MgCl2 (Scharlau MA0036: MgCl2.6H2O, MW=203.3). White precipitate will occurr, which will be dissolved after 1-2 min of stirring on magnetic stirrer at room temperature. Adjust final volume to 2 ml, divide into 0.2 ml portions and store at -80 °C.</p> | ||
<p>Final conc. in 2 ml O2k-chamber: 2-5 mM; titrate 8-20 µl using the 25 µl syringe. | <p>Final conc. in 2 ml O2k-chamber: 2-5 mM; titrate 8-20 µl using the 25 µl syringe. [[MiPNet09.12]] |
Revision as of 11:11, 10 November 2010
Description
Adenosine 5’diphosphate (D), C10H15N5O10P2K, potassium salt; substrate of ANT and ATP synthase.
Abbreviation: D
Reference: MiPNet03.02, MiPNet09.12
Labels:
Regulation: ATP; ADP; AMP; PCr"ATP; ADP; AMP; PCr" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR
D: ADP (Sigma,A 5285: Adenosine 5’diphosphate, C10H15N5O10P2K, potassium salt, MW=491.2)
Preparation of stock solution (dissolved in H2O):
# 491.2 mg/2 ml of final volume. Weigh 491.2 mg of ADP, add 1 ml H2O and 450 ul 5 N KOH. ADP will dissolve after addition of KOH. Check pH and adjust to 7 (solution A).
# 500 mM ADP stock solution: Adjust the total volume of solution A to 2 ml and divide into 0.2 ml portions. Store frozen at -80 °C.
# 500 mM ADP stock solution with 3 mM free Mg2+: To keep free [Mg2+] constant at 3 mM when titrating the ADP stock solution into MiR06, mix ADP (solution A) with MgCl2 (0.6 mol/mol ADP). Adjusting pH of ADP to 7, add 121.98 mg of MgCl2 (Scharlau MA0036: MgCl2.6H2O, MW=203.3). White precipitate will occurr, which will be dissolved after 1-2 min of stirring on magnetic stirrer at room temperature. Adjust final volume to 2 ml, divide into 0.2 ml portions and store at -80 °C.
Final conc. in 2 ml O2k-chamber: 2-5 mM; titrate 8-20 µl using the 25 µl syringe. MiPNet09.12