- high-resolution terminology - matching measurements at high-resolution
ADP
Description
Adenosine 5’diphosphate (D), C10H15N5O10P2K, potassium salt; substrate of ANT and ATP synthase.
Abbreviation: D
Reference: MiPNet03.02, MiPNet09.12,
Labels:
Regulation: ATP; ADP; AMP; PCr"ATP; ADP; AMP; PCr" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR
D: ADP (Sigma,A 5285: Adenosine 5’diphosphate, C10H15N5O10P2K, potassium salt, MW=491.2)
Preparation of 500 mM (H2O) stock solution: 491.2 mg/2 ml of final volume. Weigh 491.2 mg of ADP, add 1 ml H2O and 450 ul 5 N KOH. ADP will dissolve after addition of KOH. Check pH and adjust to 7, adjust total volume to 2 ml and divide into 0.2 ml portions. Store frozen at -80 °C.
To keep [Mg2+] constant during respiration measurement mix ADP with MgCl2 (0.6 mol/mol ADP). Preparation of Mg-ADP: After adjusting pH of ADP to 7, add 121.98 mg of MgCl2 (Scharlau MA0036: MgCl2.6H2O, MW=203.3). White precipitate will occurr, which will be dissolved after 1-2 min of stirring on magnetic stirrer. Adjust final volume to 2 ml, divide into 0.2 ml portions and store at -80 °C.
Final conc. in 2 ml O2k-chamber: 2-5 mM; titrate 8-20 µl using the 25 µl syringe. MiPNet09.12
