- high-resolution terminology - matching measurements at high-resolution
ADP
Description
Adenosine 5’diphosphate (D), C10H15N5O10P2K, potassium salt; substrate of ANT and ATP synthase.
Abbreviation: D
Reference: MiPNet03.02, MiPNet09.12
Labels:
Regulation: ATP; ADP; AMP; PCr"ATP; ADP; AMP; PCr" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
Application in HRR
D: ADP (Sigma,A 5285: Adenosine 5'diphosphate, C10H15N5O10P2K, potassium salt, FW=491.2)
Preparation of 500 mM ADP stock solution (dissolved in H2O):
- 491.2 mg/2 ml H2O of final volume (solution A)
- 1) weigh 491.2 mg of ADP (1 mmol ADP)
- 2) add 1.3 ml H2O !White precipitate will occur, which will be dissolved after 1-2 min of stirring on magnetic stirrer at room temperature!
- 3) neutralize with 5 N KOH (approx. 465 µl) !ADP will dissolve after addition of KOH!
- 4) check pH and adjust to 7 if necessary
- 5) adjust final volume to 2 ml and divide into 0.2 ml portions.
- 6) store frozen at -80 °C
Preparation of 500 mM ADP stock solution with 3 mM free Mg2+:
To keep free [Mg2+] constant during respiration measurement mix ADP (or ATP) with MgCl2 (0.6 mol/mol ADP or 0.8 mol/mol ATP).
MgCl2 (Scharlau MA0036: MgCl2.6H2O, MW=203.3)
- Solution A + MgCl2
- 1-3) follow steps 1-3 of the above instructions
- 3b) add 121,98 mg MgCl2
- 5-6) follow steps 5-6 of the above instructions
Final conc. in 2 ml O2k-chamber: 2-5 mM; titrate 8-20 µl using the 25 µl syringe. MiPNet09.12
