Difference between revisions of "Cardoso 2022 Abstract Bioblast"

Latest revision as of 08:34, 28 July 2022

4.3. «10+5» Cardoso Luiza HD, Doerrier C, Donnelly C, Komlódi T, Gnaiger E (2022) Redox monitoring and respiration - a new horizon with the NextGen-O2k. Bioblast 2022: BEC Inaugural Conference. In: https://doi.org/10.26124/bec:2022-0001

Link: Bioblast 2022: BEC Inaugural Conference

Cardoso Luiza HD, Doerrier Carolina, Donnelly Chris, Komlódi Timea, Gnaiger Erich (2022)

Event: Bioblast 2022

NADH-linked substrates (N-substrates) of TCA-cycle and other mt-matrix dehydrogenases feed electrons through the N-junction into Complex I. The redox states of the NAD-pool — defined as the sum of NAD(P)⁺ and NAD(P)H — and of the electron transfer system (ETS)-reactive coenzyme Q-pool [1] are linked in the N-pathway but are regulated independently by several convergent electron entries into the Q-junction [2]. Our results obtained with HRR and the NADH- and Q-Modules of the NextGen-O2k show that complementary to ET-pathway control of respiratory rate and redox state (redox push by electron input), coupling control exerts opposite effects on the metabolic parameters. Whereas redox push reduces the N- and Q-pool in the LEAK state at low O₂ flux and high protonomotive force pmF, stimulation of O₂ flux by ADP in the OXPHOS state [3] is accompanied by a redox pull to the oxidized state. Under these conditions, ET-pathways converging at the Q-junction yield partial additivity of O₂ flux, when ET-capacity downstream of Q and phosphorylation capacity exert flux control [2]. Monitoring of respiration together with NAD(P)H autofluorescence and Q-redox state [1] provides unique analytical and diagnostic power in the study of mitochondrial respiratory control at the N- and Q-junctions.

Komlódi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. https://doi.org/10.26124/bec:2021-0003
Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. https://doi.org/10.26124/bec:2020-0002
Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. https://doi.org/10.26124/bec:2020-0001.v1

• O2k-Network Lab: AT Innsbruck Oroboros

Affiliations and support

Oroboros Instruments GmbH, Innsbruck, Austria

This work was part of the Oroboros NextGen-O2k project, with funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement nº 859770.

List of abbreviations, terms and definitions - MitoPedia

» MitoPedia: Terms and abbreviations

Labels: MiParea: Respiration

Organism: Mouse Tissue;cell: Liver, HEK Preparation: Permeabilized cells, Isolated mitochondria

Coupling state: LEAK, OXPHOS, ET Pathway: N, S, NS, ROX HRR: Oxygraph-2k, NextGen-O2k Event: A4

@@ Line 1: / Line 1: @@
+[[File:Bioblast2022 banner.jpg|link=Bioblast_2022]]
 {{Abstract
-|title=Cardoso LHD, Gnaiger E (2022) Redox monitoring and respiration - a new horizon with the NextGen-O2k. Bioblast 2022: BEC Inaugural Conference.
+|title=4.3. '''«10+5»''' [[File:CardosoLHD.JPG|left|100px|Luiza Cardoso]] <u>Cardoso Luiza HD</u>, Doerrier C, Donnelly C, Komlódi T, Gnaiger E (2022) Redox monitoring and respiration - a new horizon with the NextGen-O2k. '''Bioblast 2022: BEC Inaugural Conference.''' In: https://doi.org/10.26124/bec:2022-0001 [[File:WatchThePresentationYoutube_icon.jpg|200px|link=https://www.youtube.com/watch?v=mNSu-hY3hUg&t=1962s|»''Watch the presentation''«]]
 |info=[https://wiki.oroboros.at/index.php/Bioblast_2022#Submitted_abstracts Bioblast 2022: BEC Inaugural Conference]
-|authors=Cardoso Luiza HD, Gnaiger Erich
+|authors=Cardoso Luiza HD, Doerrier Carolina, Donnelly Chris, Komlódi Timea, Gnaiger Erich
 |year=2022
-|abstract=NADH-linked substrates (N-substrates) of TCA-cycle and other mt-matrix dehydrogenases feed electrons through the N-junction into Complex I. The redox states of the NAD-pool — defined as the sum of NAD(P)<sup>+</sup> and NAD(P)H — and of the electron transfer system (ETS)-reactive coenzyme Q-pool are linked in the N-pathway but are regulated independently by several convergent electron entries into the Q-junction [1]. Complementary to ET-pathway control of respiratory rate and redox state (redox push by electron input), coupling control exerts opposite effects on the metabolic parameters. Whereas redox push reduces the N- and Q-pool in the LEAK state at low O<sub>2</sub> flux and high protonomotive force ''pmF'', stimulation of O<sub>2</sub> flux by ADP in the OXPHOS state [2] is accompanied by a redox pull to the oxidized state. Under these conditions, ET-pathways converging at the Q-junction yield partial additivity of O<sub>2</sub> flux, when ET-capacity downstream of Q and phosphorylation capacity exert flux control [1]. Monitoring of respiration together with NAD(P)H autofluorescence and Q-redox state [3] provides unique analytical and diagnostic power in the study of mitochondrial respiratory control at the N- and Q-junctions.
+|event=Bioblast 2022
+|abstract=NADH-linked substrates (N-substrates) of TCA-cycle and other mt-matrix dehydrogenases feed electrons through the N-junction into Complex I. The redox states of the NAD-pool — defined as the sum of NAD(P)<sup>+</sup> and NAD(P)H — and of the electron transfer system (ETS)-reactive coenzyme Q-pool [1] are linked in the N-pathway but are regulated independently by several convergent electron entries into the Q-junction [2]. Our results obtained with HRR and the NADH- and Q-Modules of the NextGen-O2k show that complementary to ET-pathway control of respiratory rate and redox state (redox push by electron input), coupling control exerts opposite effects on the metabolic parameters. Whereas redox push reduces the N- and Q-pool in the LEAK state at low O<sub>2</sub> flux and high protonomotive force ''pmF'', stimulation of O<sub>2</sub> flux by ADP in the OXPHOS state [3] is accompanied by a redox pull to the oxidized state. Under these conditions, ET-pathways converging at the Q-junction yield partial additivity of O<sub>2</sub> flux, when ET-capacity downstream of Q and phosphorylation capacity exert flux control [2]. Monitoring of respiration together with NAD(P)H autofluorescence and Q-redox state [1] provides unique analytical and diagnostic power in the study of mitochondrial respiratory control at the N- and Q-junctions.
 <small>
-# Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002
+# Komlódi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. https://doi.org/10.26124/bec:2021-0003
-# Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1
+# Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. https://doi.org/10.26124/bec:2020-0002
-# Komlódi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. Bioenerg Commun 2021.3. https://doi.org/10.26124/bec:2021-0003
+# Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. https://doi.org/10.26124/bec:2020-0001.v1
 </small>
 |mipnetlab=AT Innsbruck Oroboros
-|articletype=Abstract
 }}
 == Affiliations and support ==
@@ Line 18: / Line 19: @@
 :::: This work was part of the Oroboros [[NextGen-O2k]] project, with funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement nº 859770.
+== List of abbreviations, terms and definitions - MitoPedia ==
-== Help ==
+{{Template:List of abbreviations, terms and definitions - MitoPedia}}
-* [[MitoPedia: Terms and abbreviations]]
 {{Labeling
+|event=A4
 |area=Respiration
 |organism=Mouse