From Bioblast
NextGen-O2k
The revolutionary all-in-one instrument to conquer mitochondrial disease
Contents
Horizon 2020 Framework Programme Project NextGen-O2k
- EIC-SMEInst-2018-2020 — SME instrument phase 2, Grant Agreement No. 859770, Oroboros Instruments
- Budget: € 2.35 Million
- Duration: 24 months
- Application: 2019-01-09; Pitch in Brussels: 2019-02-15; Granted: 2019-03-05; Start: 2019-06-01
Mission
- Oroboros - as a driving force in mitochondrial physiology - extends the analytical and diagnostic power of high-resolution respirometry by integration of NADH- and Q-redox monitoring in the NextGen-O2k. We aim at establishing the Oroboros quality control management for dissemination to our worldwide O2k-Network laboratories. This will become an effective contribution to address the acute reproducibility crisis of scientific investigation. In the spirit of Open Science and global networking, we will enable data sharing across projects and institutions in an Open Access database on mitochondrial physiology and pathology, to resolve the inflation crisis and ultimately the value-impact crisis of present academic publication. This will support key developments in mitochondrial medicine. In addition, we expand our business to algal biotechnology and ecology with the photobiology module of the NextGen-O2k, widening our focus from medicine to environment and climate.
Open positions
Form the O2k to the NextGen-O2k
- Between 1992 and 1994, Oroboros Instruments released its core technology for high-resolution respirometry (HRR), the Oxygraph-2k or O2k, for real-time monitoring of oxygen consumption of mitochondria, tissues, and living cells. Dissolved oxygen is measured using high-resolution polarographic oxygen sensors. Its main advantages are the unique signal stability and resolution of oxygen. HRR is the core technology present in every single of the devices and it will remain the basis of the NextGen-O2k.
- In 2012, the updated version of the O2k was launched, the O2k-FluoRespirometer. The integrated Smart Fluo-Sensors combine fluorescence excitation and detection of defined wavelengths that in combination with fluorophores allow for the measurement of additional mitochondrial parameters, such as membrane potential, H2O2 production, calcium concentration, and ATP production.
- The unique software DatLab has been developed for data acquisition and analysis. DatLab allows the researcher to perform calibrations (including automatic barometric and temperature adjustments), background correction, experimental SUIT (substrate-uncoupler-inhibitor-titration) protocols and data analysis.
- With the O2k core technology, developed exclusively by Oroboros for high-resolution measurement of oxygen flux, the NextGen-O2k was developed.
- The NextGen-O2k is a MultiSensor all-in-one instrument for in-depth studies of (1) the mitochondrial role in human diseases, and (2) mitochondria and chloroplasts ability in regulating algal growth and metabolite production rates.
- It integrates 2 new modules: (+1) A Q-Module to measure Q-redox states, and (+2) a PB-Module to study PhotoBiology.
- In just one device researchers obtain a snapshot of the mitochondrial/chloroplast metabolic profile, saving on experimental time, reagents and samples, minimizing errors and ensuring reproducible and accurate results. In addition, the instruments offer the highest oxygen sensitivity (5 nM detection limit) with an exceptional resolution (wide oxygen concentration range, between 0-1,000 μM) and robustness (>10 years lifespan), being superior to any other market option.
O2k
- High-resolution respirometry (HRR) and unique combinations of the O2k with
- Fluorometry: the O2k-FluoRespirometer
- Potentiometry: the O2k-pH ISE-Module and O2k-TPP+ ISE-Module
- Amperometric measurement of nitric oxide: the O2k-NO Amp-Module
- High-resolution respirometry (HRR) and unique combinations of the O2k with
NextGen-O2k
- Extend high-resolution respirometry (HRR) to the all-in-one device
- (+1) Cyclic voltammetry: the Q-redox sensor
- (+2) Controlled wavelengths and light intensity: PhotoBiology-Module
- Save on experimental time, reagents and samples
- Minimize errors and ensure reproducible and accurate results
- Highest oxygen sensitivity (5 nM detection limit)
- Exceptional resolution (wide oxygen concentration range, between 0 - 1,000 μM)
- Robustness (>10 years lifespan)
- Extend high-resolution respirometry (HRR) to the all-in-one device
Partners
- Our partners WGT-Elektronik and SHTech have been essential in the development of the NextGen-O2k.
- Early testers
- The influence of KOLs and Early testers/adopters will be used to build awareness of NextGen-O2k among researchers, academic and industrial partners. We have confirmed relevant mitochondrial and algal biotechnology researchers who want to collaborate as early testers:
- Prof. Anthony Moore
- Prof. Massimo Zeviani
- Dr. Carlo Viscomi
- Prof. Pablo Garcia-Roves
- Prof. Christos Chinopoulos
- Dr. Anthony Molina
- Dr. Marten Szibor
- Prof. Howard Jacobs
- Prof. Mervyn Singer
- Prof. Tom Bibby
- Dr. Tomas Morosinotto
- Prof. Claire Remacle
- Dr. Yagut Allahverdiyeva
- Prof Ted Packard
Workpackages
- WP1
- » Technical developments
- WP1
- WP2
- » Scientific developments
- WP2
- WP3
- » Documentation
- WP3
- WP4
- » Exploitation
- WP4
- WP5
- » Dissemination
- WP5
- WP6
- » Project management
- WP6
Events
| When | Where | |
|---|---|---|
| OroDM01 Innsbruck AT | 2019-07-01 |  |
| MiPNet24.02 IOC141 Schroecken AT | 2019-09-23 | |
Gentle Science
- In addition to O2k-Workshops and on-site training, Oroboros will disseminate the not-for-profit initiatives on Mitochondrial Physiology and Bioenergetics Science Camps, specialized Mitochondrial Physiology Conferences, and Preprint publications:
- » Oroboros is a key player in support of the COST Action MitoEAGLE
- » MitoGlobal - the world-wide platform for societies and organizations supporting mitochondrial research and medicine
- » MitoEAGLE with cooperation by Oroboros launched MitoFit Preprint Archives - the preprint server for mitochondrial physiology and bioenergetics
- » A vision on preprints
- » Mitochondrial respiratory states and rates : >530 coauthors join forces towards harmonization on nomenclature
- » Gentle Science - recognizes the responsibility of the scientific community - for the quality of science, the quality of life in science, and its mission
- In addition to O2k-Workshops and on-site training, Oroboros will disseminate the not-for-profit initiatives on Mitochondrial Physiology and Bioenergetics Science Camps, specialized Mitochondrial Physiology Conferences, and Preprint publications:
