Difference between revisions of "Coupling/pathway control diagram"
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|abbr=CPCD | |abbr=CPCD | ||
|description=[[File:SUIT-nomenclature.jpg|300px|right|SUIT protocols]] | |description=[[File:SUIT-nomenclature.jpg|300px|right|SUIT protocols]] | ||
'''Coupling-pathway control diagrams''' illustrate the respiratory '''states''' obtained step-by-step in substrate-uncoupler-inhibitor titrations in a [[SUIT protocol]]. Each step (the next state) is defined by an initial state and a [[metabolic control variable]], ''X''. The respiratory states are shown by boxes. ''X'' is usually the titrated substance in a SUIT protocol. If ''X'' (such as ADP or an uncoupler) exerts '''coupling control''', then a transition is induced between two [[coupling control state]]s. If ''X'' (such as pyruvate | '''Coupling-pathway control diagrams''' illustrate the respiratory '''states''' obtained step-by-step in substrate-uncoupler-inhibitor titrations in a [[SUIT protocol]]. Each step (to the next state) is defined by an initial state and a [[metabolic control variable]], ''X''. The respiratory states are shown by boxes. ''X'' is usually the titrated substance in a SUIT protocol. If ''X'' (such as ADP or an uncoupler) exerts '''coupling control''', then a transition is induced between two [[coupling control state]]s. If ''X'' (such as the fuel substrates pyruvate and succinate, or the inhibitor rotenone) exerts '''pathway control''', then a transition is induced between two [[pathway control state]]s. The type of metabolic control (''X'') is shown by arrows linking two respiratory states, with vertical arrows indicating coupling control, and horizontal arrows indicating pathway control. [[Marks in DatLab]] define the section of an experimental trace in a given [[respiratory state]] (steady state). [[Events in DatLab]] define the titration of ''X'' inducing a transition in the SUIT protocol. The specific sequence of coupling control and pathway control steps defines the [[SUIT protocol pattern]]. The coupling-pathway control diagrams define the [[categories of SUIT protocols]] (see Figure). | ||
|info=[[Gnaiger 2014 MitoPathways]] | |info=[[Gnaiger 2014 MitoPathways]] | ||
}} | }} |
Revision as of 11:13, 31 October 2016
- high-resolution terminology - matching measurements at high-resolution
Coupling/pathway control diagram
Description
Coupling-pathway control diagrams illustrate the respiratory states obtained step-by-step in substrate-uncoupler-inhibitor titrations in a SUIT protocol. Each step (to the next state) is defined by an initial state and a metabolic control variable, X. The respiratory states are shown by boxes. X is usually the titrated substance in a SUIT protocol. If X (such as ADP or an uncoupler) exerts coupling control, then a transition is induced between two coupling control states. If X (such as the fuel substrates pyruvate and succinate, or the inhibitor rotenone) exerts pathway control, then a transition is induced between two pathway control states. The type of metabolic control (X) is shown by arrows linking two respiratory states, with vertical arrows indicating coupling control, and horizontal arrows indicating pathway control. Marks in DatLab define the section of an experimental trace in a given respiratory state (steady state). Events in DatLab define the titration of X inducing a transition in the SUIT protocol. The specific sequence of coupling control and pathway control steps defines the SUIT protocol pattern. The coupling-pathway control diagrams define the categories of SUIT protocols (see Figure).
Abbreviation: CPCD
Reference: Gnaiger 2014 MitoPathways
MitoPedia concepts:
MiP concept,
SUIT concept
Communicated by Gnaiger Erich 2016-06-07, edited 2016-10-31.