Guzun 2011 Biochim Biophys Acta: Difference between revisions
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Revision as of 17:13, 7 November 2016
| Guzun R, Karu-Varikmaa M, Gonzalez-Granillo M, Kuznetsov AV, Michel L, Cottet-Rousselle C, SaaremΓ€e M, Kaambre T, Metsis M, Grimm M, Auffray C, Saks V (2011) Mitochondriaβcytoskeleton interaction: Distribution of Ξ²-tubulins in cardiomyocytes and HL-1 cells. Biochim Biophys Acta 1807:458-69. |
Guzun R, Karu-Varikmaa M, Gonzalez-Granillo M, Kuznetsov AV, Michel L, Cottet-Rousselle C, Saaremaee M, Kaambre T, Metsis M, Grimm M, Auffray C, Saks V (2011) Biochim Biophys Acta
Abstract: Mitochondria-cytoskeleton interactions were analyzed in adult rat cardiomyocytes and in cancerous non-beating HL-1 cells of cardiac phenotype. We show that in adult cardiomyocytes Ξ²II-tubulin is associated with mitochondrial outer membrane (MOM). Ξ²I-tubulin demonstrates diffused intracellular distribution, Ξ²III-tubulin is colocalized with Z-lines and Ξ²IV-tubulin forms microtubular network. HL-1 cells are characterized by the absence of Ξ²II-tubulin, by the presence of bundles of filamentous Ξ²IV-tubulin and diffusely distributed Ξ²I- and Ξ²III-tubulins. Mitochondrial isoform of creatine kinase (MtCK), highly expressed in cardiomyocytes, is absent in HL-1 cells. Our results show that high apparent K(m) for exogenous ADP in regulation of respiration and high expression of MtCK both correlate with the expression of Ξ²II-tubulin. The absence of Ξ²II-tubulin isotype in isolated mitochondria and in HL-1 cells results in increased apparent affinity of oxidative phosphorylation for exogenous ADP. This observation is consistent with the assumption that the binding of Ξ²II-tubulin to mitochondria limits ADP/ATP diffusion through voltage-dependent anion channel of MOM and thus shifts energy transfer via the phosphocreatine pathway. On the other hand, absence of both Ξ²II-tubulin and MtCK in HL-1 cells can be associated with their more glycolysis-dependent energy metabolism which is typical for cancer cells (Warburg effect).
β’ O2k-Network Lab: EE Tallinn Kaambre T, EE Tallinn Saks VA, FR Grenoble Saks VA, FR Grenoble Schlattner U
Labels: MiParea: Respiration, mt-Structure;fission;fusion
Organism: Rat
Tissue;cell: Heart
Preparation: Isolated mitochondria
Coupling state: OXPHOS
Pathway: N
HRR: Oxygraph-2k
