MiPNet11.02 IOC32

                

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MiPNet11.02 IOC32

Schroecken AT, 2006 Apr 21-25. : Oroboros O2k-Workshop on high-resolution respirometry (HRR): O2k, TIP-2k and DatLab 4, IOC32.

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Oroboros (2006-04-21) Mitochondr Physiol Network

Abstract: O2k-International course on high-resolution respirometry: Oroboros O2k, TIP-2k and DatLab 4. Schroecken, Voralberg, Austria; 2006 April 21-25.

>> O2k-Workshop: Current dates

>> Product: Oroboros O2k, O2k-Catalogue

• O2k-Network Lab: AT_Innsbruck_Oroboros

Labels:

ORO, IOC, 2006 

Lecturers and tutors

Gnaiger Erich, CEO, Oroboros Instruments

Susana Cadenas, O2k-Network Lab: Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM) and Departamento de Biología Molecular

Krivakova-Stankova Pavla, O2k-Network Lab: Charles University in Prague

Braun Valerie, O2k-Network Lab: Oroboros Instruments

Participants

Participant	Institution
Brasseur Gael	FR Marseille Brasseur G: Laboratoire de Bioénergétique et Ingénierie des Protéines (FR)
Cleary Joanne	US MI Ann Arbor Glick GD: University of Michigan (US)
Galkin Alexander	UK Belfast Galkin A: Queen's University Belfast (UK)
Grégori Gérald	FR Marseille Gregori G: Laboratoire de Microbiologie, Géochimie et Ecologie Marines (FR)
Hansen Lillian Lund	DK Copenhagen Quistorff B: Dept. of Medical Biochemistry and Genetics, University of Copenhagen (DK)
How Ole-Jakob	NO Tromsoe Larsen TS: Department of Medical Physiology, Institute of Medical Biology, Faculty of Medicine, University of Tromsø (NO)
Hunger Miriam	AT Innsbruck Oroboros (AT)
Keeney Paula M	US VA Richmond Bennett JP: Virginia Commonwealth University (US)
Larsen Terje S.	NO Tromsoe Larsen TS: Department of Medical Physiology, Institute of Medical Biology, Faculty of Medicine, University of Tromsø (NO)
Lenaers Ellen	NL Maastricht Schrauwen P: Department of Human Biology, Maastricht University (NL)
Lopez Anna	ES Granada Acuna-Castroviejo D: Centro de Investigación Biomédica, Parque Tecnológico de Ciencias de la Salud, Universidad de Granada (ES)
Palacino James	US MA Cambridge Liang J (US)
Petrov Svetlana	SE Molndal Hallen S (SE)
Phielix Esther	NL Maastricht Schrauwen P: Department of Human Biology, Maastricht University (NL)
Sauerwein Hand P.	NL Amsterdam Serlie MJ: Academic Medical Center, University of Amsterdam, Department of Endocrinology and Metabolism (NL)
Scheibye-Knudsen Morten	US MD Baltimore Scheibye-Knudsen M (US)
Schoonderwoerd Kees GC	NL Rotterdam Sluiter W, NL Rotterdam Schoonderwoerd KGC: Department of Biochemistry (NL)
Serlie Mireille	NL Amsterdam Serlie MJ: Academic Medical Center, University of Amsterdam, Department of Endocrinology and Metabolism (NL)
Sluiter Wim	NL Rotterdam Sluiter W: Department of Biochemistry (NL)
Subarsky Patrick	University of Toronto (CA)
Swenson Lara	US MI Ann Arbor Glick GD: University of Michigan (US)
Therkelsen Arent Ib	DK Copenhagen Quistorff B: Dept. of Medical Biochemistry and Genetics, University of Copenhagen (DK)

Hot topics in mitochondrial physiology - abstracts

• Look the abstracts >>>

	Has title
Cadenas 2006 Biochim Biophys Acta	Aguirre E, Rodriguez-Juarez F, Gnaiger E, Cadenas S (2006) Measurement of the control of cellular respiration by nitric oxide under normoxia and hypoxia: instrumental comparison including high-resolution respirometry. Biochim Biophys Acta, EBEC Short Reports Suppl 14:136-7.

Feedback

• Thank you for the slides of the April 2006 O2K Course. They remind me of what a wonderful time all of us had together! We are delighted with our O2k! It is obviously a very sensitive piece of equipment and the DatLab software makes using it very easy. The respiratory state of our cell lines will be important information for our group. I must be honest though, with no background in respirometry or with the O2k, I would have been lost without all the very helpful instruction I received in Shrocken. Thank you for making our use of this fine machine possible! - Keeney Paula M, O2k-Network Lab: US VA Richmond Bennett JP

• First of all, thank you, everybody, for a most pleasant meeting in Schröcken! Concerning some details of the Oxygraph time derivative, I have performed a small experiment called "Stirrer.dld", from which two Excel-files, "Stirrer-A" and "Stirrer-B" evolves. The Excel-files are more or less identical, simply Chamber A and B on the same instrument. The following references to figures concerns the file "Stirrer-A". Oxygraph users have noticed, that upon rapid changes in Oxygen concentration (as seen by switching the stirrer on and off) the Oxygraph time derivative seems to lag behind (Fig.1, yellow line), and, as pointed out by Alexander G., even stretches to a much longer time span than the duration of the oxygen changes themselves would suggest. (Fig.2, yellow line) On discussing this topic with Erich G., it appears that the Oxygraph employs a calculation procedure which, at any time point, uses the preceeding 40 data points to determine the time derivative. The procedure also seems to introduce a good deal of smoothing, and for most pracical purposes, in which equilibration evolves over a slow time span of minutes, the built-in time derivative is robust and easy to read. However, if need be, there are other ways of calculating the time derivative. For comparison with the Oxygraph, I have used a procedure called "Deriv" which is an inherent part of software "IDL" (Interactive Data Language). Code for Deriv is attached to this mail as well for the benefit of anyone curious. It is a 3-point Lagrange numerical derivative, which closely follows the Oxygen changes in time, but the outcome is far more noisy than the Oxygraph procedure. - Therkelsen Arent Ib, O2k-Network Lab: DK Copenhagen Quistorff B

• Thanks a lot for the lovely time we had at the Oroboros respiration course. I came back to Sweden and today is my second working day. We started to talk about the first experiments with the Oroboros instrument and decided that the best way to start will to repeat the PCT-protocol with the cell suspension of 32D cells according to the protocol we got from the course. - Petrov Svetlana, O2k-Network Lab: SE Molndal Hallen S

• I would like to thank you for the excellent respirometry course you have organized. - Galkin Alexander, O2k-Network Lab: UK Belfast Galkin A

• I wanted to thank you for a very informative and fun O2k Course last week. - Swenson Lara, O2k-Network Lab: US MI Ann Arbor Glick GD

• Thanks again for your organization of the High-Resolution Respirometry workshop. As you already know, I thoroughly enjoyed myself, learning a great deal of science and enjoying the mountain air. - Subarsky Patrick, O2k-Network Lab: University of Toronto

• This week in Austria has been fabulous. It's been the first time that I´ve left Spain for working and it wasn´t really an effort, just the opposite, I´ve enjoyed a lot and I´m glad we´ve come. Thank you very much for being so friendly and kindly with us. Now this week is part of the good old days of my life. Thanks for everything. - Lopez Anna, O2k-Network Lab: ES Granada Acuna-Castroviejo D

• Thanks a lot for all the efforts you have done for the fabulous workshop in Schrocken. There is a nice collection of photos which reflects not only our "hard work" we have done with the Oxygraph-2k but also the very good time we have spent in the mountains and at the bar...discussing "always" about respiration, uncoupling with alcohol, and also sometimes O2 kinetics for high affinity terminal bd oxidases in funny bugs.. Thanks a lot and I will recommand your machine and the nice place in Schroecken to my colleagues in the field, specially the ones who still use the prehistoric gilson machine.. All the best, - Brasseur Gael, O2k-Network Lab: FR Marseille Brasseur G

• I am back to the lab and want to thank you one more time for this wonderfull workshop. It was great and the people were so nice. - Grégori Gérald, O2k-Network Lab: FR Marseille Gregori G

Impressions from the IOC32

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O2k-Workshops are listed as MitoGlobal Events.