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| |year=2015-05-15 | | |year=2015-05-15 |
| |journal=Mitochondr Physiol Network | | |journal=Mitochondr Physiol Network |
| |abstract=Gnaiger E, Fasching M, Gradl L, Gradl P (2015) Oxygraph-2k: start high-resolution respirometry. Mitochondr Physiol Network 19.18(A02):3-16. | | |abstract=Gnaiger E, Fasching M, Gradl L, Gradl P (2015) Oxygraph-2k: start high-resolution respirometry. Mitochondr Physiol Network 19.18(A02):3-16. |
| | '''Videosupport''': Links to video clips are functional on the USB flash drive of the [[O2k-Compendium]]. |
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| The OROBOROS [[Oxygraph-2k]] provides a unique high-resolution approach to the monitoring of cellular and mitochondrial respiratory function. [[High-resolution respirometry]] is indispensable at low respiratory activities, kinetic transitions and low oxygen levels. Such high resolution is required in particular for the analysis of pathological effects resulting in reduced respiration (mitochondrial and metabolic diseases, ischemia-reperfusion injury, oxidative stress, apoptosis, aging); biopsies with limited amounts of sample (diagnosis of genetic and acquired mitochondrial defects in pediatric patients, diabetes); cell cultures with limited cell numbers or low activities; mutants with diminished respiratory capacity; chemical oxidation rates and antioxidant capacities; and respiratory measurements at low, physiological intracellular oxygen levels and oxygen kinetics.
| | :» Product: [[Oxygraph-2k]], '''[[DatLab]] 6''', [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] |
| High-resolution respirometry by the OROBOROS Oxygraph-2k is based on a radically different systems approach to polarographic measurement of oxygen dynamics. High-resolution respirometry rests on several tightly connected instrumental features and concepts integrated by the DatLab software, providing on-line display of oxygen concentration and respiration ([[oxygen flux]]). The mechanics, critical selection of materials, and state-of-the-art electronics provide the basis of signal stability of an optimized [[polarographic oxygen sensor]], in an electronically controlled thermal environment with high temperature stability. Calibrations and instrumental controls follow standardized procedures, with highly automatic on-line analysis by the DatLab software. Scientific support is complementary to the approach of high-resolution respirometry.
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| While the OROBOROS Oxygraph-2k provides the instrumental basis for high-resolution respirometry, successful operation at high accuracy up to the limit of detection depends on a professional application by the technician, scientist or student. Participation in a hands-on introductory [[O2k-Workshop]] is recommended to save time and gain from the experience obtained over many years of application in various settings.
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| Links to video clips are functional on the USB flash drive of the [[O2k-Compendium]].
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| :» Product: '''[[DatLab]] 6''', [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]
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| |keywords=HRR | | |keywords=HRR |
| |mipnetlab=AT_Innsbruck_OROBOROS | | |mipnetlab=AT_Innsbruck_OROBOROS |
| }} | | }} |
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| {{Labeling | | {{Labeling |
| |area=Instruments;methods | | |area=Instruments;methods |
Revision as of 18:51, 19 May 2015
