MitoEAGLE data: muscle

News and Events

Working Groups

Short-Term Scientific Missions

Management Committee

Members

---

---

COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping

MitoEAGLE data: muscle

WG2

MITOEAGLE data repository in muscle and other tissues

WG2 Description

During the past 10 years, a dramatically increasing number of studies report respirometric data on human muscle tissue (e.g. Christiansen 2015 Am J Physiol). Muscle mitochondrial function is particularly studied in genetically engineered mouse models of human diseases (e.g. Jelenik 2014 Diabetes). However, despite the many published data, lack of consistency often precludes quantitative comparison among data sets with permeabilized or homogenized muscle fibres or isolated mitochondria, normalized to either tissue wet weight, dry weight, protein content, citrate synthase activity or other mitochondrial markers. In the absence of validated conversion factors, these data remain largely unconnected and can only be qualitatively linked.

The aim of WG2 will be to provide access to complete data sets for post-study statistical analysis and use harmonization tools (WG1) for standardized documentation, to initiate a data repository on muscle tissue from humans and model organisms in health and disease. Data sets shall be obtained from already published and new studies, prompting participating labs to report experimental details beyond those already published as far as possible (using tools such as PubMed Commons). As a result, specific protocols will be summarized for handling muscle tissue. Depending on specific interests, WG2 may decide to extend their focus to other tissue types.

WG2 Participants

Country	City	Contact
Australia	Melbourne	Bishop David J
Austria	Innsbruck	Gnaiger Erich
Austria	Salzburg	Mayr JA
Austria	Innsbruck	Doerrier Carolina
Austria	Innsbruck	Laner Verena
Belgium	Liege	Votion Dominique-Marie
Belgium	Leuven	Bird Matthew
Brazil	São Paulo	Ferreira Julio Cesar B
Brazil	Jaboticabal, SP	Garcia Geovana S
Canada	Edmonton (Alberta)	Lemieux Helene
Canada	Rimouski	Blier Pierre U
Canada	Antigonish	Kane Daniel A
Canada	Rimouski	Vandermeeren E
Canada	Vancouver	Boushel Robert C
Croatia	Zagreb	Saric A
Croatia	Split	Mihanovic I
Czech Republic	Prague 10	Krajcova Adela
Czech Republic	Pilsen	Kuncova J
Czech Republic	Hradec Králové	Waldauf P
Czech Republic	Pilsen	Markova Michaela
Czech Republic	Prague	Elkalaf Moustafa
Denmark	Copenhagen N	Mortensen OH
Denmark	Copenhagen	Larsen Steen
Denmark	Hvidovre	Nehlin Jan O
Denmark	Copenhagen	Gonzalez-Franquesa Alba
Denmark	Malov	Hey-Mogensen M
Denmark	Copenhagen	Dela Flemming
Estonia	Tallinn	Kaambre Tuuli
Estonia	Tallinn	Vendelin Marko
Estonia	Tallinn	Tepp Kersti
Estonia	Tallinn	Aid-Vanakova Jekaterina
Estonia	Tallinn	Karro N
Estonia	Tartu	Paju Kalju
Estonia	Tallinn	Puurand Marju
Estonia	Tallinn	Truu Laura
Finland	Helsinki	Pirinen E
Finland	Jyvaeskylae	Lehti Maarit
France	Bordeaux cedex	Mourier A
France	Grenoble Cedex 9	Schlattner Uwe
France	Grenoble cedex 9	Dubouchaud Herve
France	Montpellier Cedex 1	Chabi Beatrice
France	Paris	Ventura-Clapier R
France	Montpellier	Cortade F
France	Paris	Armand Anne-Sophie
France	Paris	Petit Patrice X
Germany	Leipzig	Ost Mario
Germany	Rostock	Haider F
Germany	Cologne	Pesta Dominik
Germany	Nuthetal	Klaus S
Germany	Munich	Jastroch M
Germany	Bochum	Platen P
Germany	Düsseldorf	Granata Cesare
Germany	Köln	Kimoloi Sammy
Germany	Duesseldorf	Jelenik T
Germany	Duesseldorf	Roden Michael
Germany	Bremerhaven	Mark Felix Christopher
Greece	Heraklion	Tavernarakis Nektarios
Greece	Heraklio	Sidiropoulou K
Greece	Athens	Trougakos Ioannis P
India	Haryana	Sharma Vipin
Ireland	Dublin	O'Gorman Donal
Ireland	Dublin	Porter Richard K
Ireland	Sligo	Murphy J
Israel	Rishon Le Zion	Hachmo Yafit
Israel	Zrifin	Sova Marina
Israel	Jerusalem	Saada Reisch Ann
Italia	Udine	Salvadego Desy
Italy	Pisa	Gastaldelli Amalia
Italy	Rome	Scatena Roberto
Italy	Novara	Reano Simone
Italy	Milan	De Palma Clara
Italy	Milan	Giovarelli Matteo
Italy	Bologna	Genova Maria Luisa
Japan	Sapporo	Nambu H
Japan	Sapporo	Maekawa S
Japan	Sapporo	Shirakawa R
Latvia	Riga	Kuka Janis
Latvia	Riga	Zvejniece Liga
Latvia	Riga	Inashkina I
Latvia	Riga	Liepins Edgars
Luxembourg	Strassen	Devaux Yvan
Malta	Msida	Vella Joanna
Netherlands	Maastricht	Hoeks Joris
Netherlands	BT Amsterdam	Jaspers Richard T
Netherlands	Maastricht	Van Polanen N
Netherlands	Wageningen	Keijer Jaap
Netherlands	Amsterdam	De Goede Paul
Netherlands	Maastricht	Grevendonk L
Niederlande	ER Masstricht	De Wit-Verheggen V
Niederlande	ER Masstricht	De Theije C
Norway	Bergen	Tronstad Karl Johan
Norway	Oslo	Eide L
Norway	Tromsoe	Schots Pauke C
Norway	Tromsoe	Larsen Terje S
Norway	Ullevål	Heiestad Christina
Norway	Oslo	Lund Jenny
Norway	Tromsoe	Aasum E
Norway	Oslo	Nikolic N
Norway	Oslo	Torp May-Kristin
Norway	Tromsoe	Jansen Kirsten M
Norway	Oslo	Rustan AC
Poland	Warsaw	Sek Aleksandra
Poland	Warsaw	Kampa Rafal Pawel
Poland	Warsaw	Dymkowska Dorota
Poland	Lodz	Watala Cezary
Poland	Lodz	Labieniec-Watala Magdalena
Portugal	Cantanhede	Martins Joao D
Portugal	Cantanhede	Ferreira LL
Portugal	Coimbra	Martins Marques T
Portugal	Coimbra	Palmeira Carlos
Portugal	Porto	Coxito Pedro
Portugal	Porto	Magalhaes Jose
Portugal	Coimbra	Carvalho Eugenia
Portugal	Coimbra	Pereira Susana P
Portugal	Porto	Beleza Jorge
Romania	Timisoara	Lighezan R
Romania	Timisoara	Muntean Danina M
Singapore	Singapore	Hausenloy Derek J
Slovakia	Bratislava	Ukropcova B
Slovakia	Bratislava	Maderova D
Slovakia	Bratislava	Nemec Michal
Slovakia	Bratislava	Ukropec J
Slovakia	Bratislava	Sumbalova Zuzana
Slovenia	Ljubljana	Pirkmajer Sergej
Slovenia	Ljubljana	Dolinar K
Slovenia	Ljubljana	Mars T
South Africa		Smith James
Spain	Granada	Lopez Garcia Luis Carlos
Spain	Madrid	Moran M
Spain	Barcelona	Sebastian D
Spain	Madrid	Cadenas S
Spain	Barcelona	De la Torre Lara J
Spain	Sevilla	Bustos Matilde
Spain	Barcelona	Catalan M
Spain	Barcelona	Zorzano Antonio
Spain	Barcelona	Batlle M
Spain	Armilla (Granada)	Fernandez-Ortiz Marisol
Spain	Armilla (Granada)	Acuna-Castroviejo Dario
Spain	Barcelona	Gama Perez Pau
Spain	A Coruña	Blanco FJ
Spain	Barcelona	Guitart M
Spain	Barcelona	Garcia-Roves Pablo Miguel
Spain	Armilla, Granada	Escames Germaine
Sweden	Linköping	Fontes-Oliveira CC
Sweden	Stockholm	Cardinale Daniele A
Switzerland	Lausanne	Zalachoras I
Switzerland	Lausanne	Gebara E
Switzerland	Lausanne	Morato Fornaguera L
Switzerland	Lausanne	Sandi Carmen
Switzerland	Bern	Nuoffer Jean-Marc
Switzerland	Lausanne	Weger M
The Netherlands	Wageningen	Van der Ende Miranda
The Netherlands	BT Amsterdam	Wuest Rob CI
Turkey	Ankara	Tuncay Erkan
USA	Chicago	Dayanidhi Sudarshan
USA	Fairbanks	O'Brien Kristin
USA	Torrance	Rossiter Harry B
USA	Baton Rouge	Irving Brian A
USA	Baltimore	Gonzalez-Freire Marta
USA	Fort Collins	Chicco Adam J
USA	College Station	White-Springer Sarah H
USA	Baltimore	Fang Evandro F
USA	Colorado Springs	Jacobs RA
USA	Norfolk	Lai Nicola
USA	Ann Arbor	Beard Daniel A
USA	Gainesville	Hepple Russell T
USA	Boca Raton	Khamoui Andy V
USA	Orlando	Distefano Giovanna
USA	St Louis	Welch R
USA	Gainesville	Neyroud D
USA	College Station	Latham C
USA	Baton Rouge	Axelrod Christopher L
USA	Little Rock	Porter Craig
USA	Gainesville	Wohlgemuth Stephanie E
USA	Orlando	Coen Paul M
United Kingdom	London	Szabadkai Gyorgy
United Kingdom	Loughborough	Ebanks Brad
United Kingdom	Birmingham	Thakker A
United Kingdom	Birmingham	Garten Antje
United Kingdom	Plymouth, Devon	McKenna Helen T
United Kingdom	Cambridge	Shiroma Jonathan G
United Kingdom	Nottingham	Chakrabarti Lisa
United Kingdom	Brighton	Moore Anthony L
United Kingdom	Birmingham	Ashcroft S
United Kingdom	Leeds	Boyle JP
United Kingdom	Cambridge	Murray Andrew James
United Kingdom	Oxford	Makrecka-Kuka Marina
United Kingdom	Birmingham	Joanisse S
United Kingdom	Manchester	Giakoumaki I
United States	Providence	Siamwala J

WG2 Management

Tasks

1. Call for participating labs to deliver relevant experimental data on already published studies and to report them in a standardized format.
2. Collection and indexing of the data sets as required for statistical analysis.
3. Discussion of procedures, protocols and general guidelines.
4. Draft a comprehensive review on mt-function in muscle tissue (registration at PROSPERO, link with EQUATOR).
5. Opening of the database to the research public for data search and moderated entry of new data sets.

Milestones

1. Call for data report delivered.
2. Data sets entered in standardized format.
3. Summary, interpretation and discussion of data for review.

Deliverables

1. A MITOEAGLE database on muscle tissue from humans and model organisms.
2. A set of guidelines for future studies on mitochondrial bioenergetics in muscle tissue.
3. Draft of review manuscript.

WG2 Feedback

1. Skeletal muscle is the main tissue we are studying in the lab. - Beatrice Chabi (2016).
2. For the kind of work group, our expertise is certainly most on muscle tissue (skeletal and heart), although we also worked on all kinds of permeabilized cells (primary cells and cell lines). For all these, we have extended data sets, mainly rat, but also human, from diverse experimental setups (nutritional regimen, exercise, drugs ...). - Uwe Schlattner (2016).
3. I would fit rather in WG1, as myself I am not involved in human muscle fat or blood. However, in our team we made a study involving human liver samples. - Frédéric Bouillaud (2016).
4. In my working group we are mainly interested in muscle, especially focusing on aging and sarcopenia (using animal models). I would be happy if new cooperations could be formed through the COST Action. I could imagine working on a joint review article. Next to examining muscle from mouse models, we are studying biopsies from human heart muscle and liver, but there is no published data on these studies yet. - Susanne Klaus (2016). Translated by Sandra Fleischmann
5. I am very interested in being part of the management committee either as a MC member for managment tasks or in working group 4 MITOEAGLE data repository for blood cells and cultured cells. I have experience in analyzing mitochondrial function in different human tissues as cultured cells and blood cells (also subsets of specific blood populations, see list of publications), muscle and isolated mitochondria. This together with my experience in organizing summer schools and conferences could be helpful as MC member taking care of management tasks. However, if you think my expertise is more helpful in working group 4, I am looking forward to support this working group. - Kathrin Renner-Sattler (2016).
6. I am working in the field of mitochondrial biochemistry since many years. In detail my coworkers and I are carrying out studies on the regulation of ATP synthase and respiratory complexes in swine heart mitochondria as biological model of mammalian mitochondria. Some comparative aspects of mitochondrial bioenergetics and ATP synthase responses are also investigated in bivalve mollusk mitochondria. - Alessandra Pagliarani (2016).

Projects in progress

» MiPMap