Difference between revisions of "NextGen-O2k Scientific developments"
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::::'''Project Year 1:'''Β During testing in the first project year, optimal measurement conditions were determined for using the Q-sensor with a variety of biological samples. Simultaneously testing began of the beta version of DatLab. New SUIT protocols were developed for general, pre-clinical, and algal biotechnology applications. A focus has also been given to internal and external quality control measures. | ::::'''Project Year 1:'''Β During testing in the first project year, optimal measurement conditions were determined for using the Q-sensor with a variety of biological samples. Simultaneously testing began of the beta version of DatLab. New SUIT protocols were developed for general, pre-clinical, and algal biotechnology applications. A focus has also been given to internal and external quality control measures. | ||
::::'''Project Year 2:''' In the second year of the project, experiments with microalgae began, including determining the process for permeabilization and SUIT protocol development. Two preprints were published on microalgae as a result of this work. A further preprint was published on data from Q-Module experiments about the coupling and pathway control of coenzyme Q redox state and respiration. In addition to these developments, NADH-Module testing also started. | |||
== Progress and next steps == | == Progress and next steps == | ||
See Β» [[Talk:NextGen-O2k Scientific developments|Track record]] | |||
<br/> | |||
<gallery mode=default perrow=4 widths="350px" heights="300px"> | |||
File:Luiza Q-module testing and DatLab8.jpg|[[Cardoso Luiza HD|Luiza Cardoza, PhD.]] testing the Q-Module and DatLab8 | |||
File:Microalgae for testing PB module.jpg|Microalgae for testing PB module | |||
|- | |||
: | </gallery> | ||
== NextGen-O2k MitoFit Preprints == | |||
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Revision as of 10:26, 6 May 2021
Template:MitoFit highlights page name
The revolutionary all-in-one instrument to conquer mitochondrial disease.
WP2: Scientific developments
Abstract
- The aim of this workpackage is to test measurement conditions for the newly developed modules and the development of specific applications.
- Determination of optimal Q-sensor measurement conditions
- Determination of optimal measurement conditions for the PB-Module
- Development of protocols for scientific, pre-clinical and algal biotechnology applications
- Data handling and quality control
- The aim of this workpackage is to test measurement conditions for the newly developed modules and the development of specific applications.
Achievements
- Project Year 1: During testing in the first project year, optimal measurement conditions were determined for using the Q-sensor with a variety of biological samples. Simultaneously testing began of the beta version of DatLab. New SUIT protocols were developed for general, pre-clinical, and algal biotechnology applications. A focus has also been given to internal and external quality control measures.
- Project Year 2: In the second year of the project, experiments with microalgae began, including determining the process for permeabilization and SUIT protocol development. Two preprints were published on microalgae as a result of this work. A further preprint was published on data from Q-Module experiments about the coupling and pathway control of coenzyme Q redox state and respiration. In addition to these developments, NADH-Module testing also started.
Progress and next steps
See Β» Track record
Luiza Cardoza, PhD. testing the Q-Module and DatLab8
NextGen-O2k MitoFit Preprints
Link | View | Reference | Year |
---|---|---|---|
Went 2021 MitoFit PB | MitoFit Preprints 2021.05. | Went N, Di Marcello M, Gnaiger E (2021) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry. MitoFit Preprints 2021.05. https://doi.org/10.26124/mitofit:2021-0005 | 2021 |
Huete-Ortega 2020 MitoFit Preprint Arch EA | MitoFit Preprint Arch EA20.1. High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii β towards biotechnology exploitations | Huete-Ortega M, Di Marcello M, Iglesias-Gonzalez J, Gnaiger E (2020) High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii β towards biotechnology exploitations. https://doi.org/10.26124/mitofit:ea20.algaeurope.0001 | 2020 |
Donnelly 2023 MitoFit | MitoFit Preprints 2023.2. Functional hypoxia reduces mitochondrial calcium uptake. | Donnelly C, KomlΓ³di T, Cecatto C, Cardoso LHD, Compagnion AC, Matera A, Tavernari D, Zanou N, Kayser B, Gnaiger E, Place N (2023) Functional hypoxia reduces mitochondrial calcium uptake. MitoFit Preprints 2023.2. https://doi.org/10.26124/mitofit:2023-0002 β 2024-11-17 published in Redox Biol. | 2023 |