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OctPM pathway control state

From Bioblast


high-resolution terminology - matching measurements at high-resolution


OctPM pathway control state

Description

PMOct: Pyruvate & Malate & Octanoylcarnitine.

MitoPathway control: CI&FAO

SUIT protocol: SUIT-RP1, SUIT-RP1

This substrate combination supports CI-linked flux which is typically higher than FAO capacity. In SUIT-RP1, PMOct is induced after PM(E), to evaluate any additive effect of adding Oct. In SUIT-RP2, FAO OXPHOS capacity is measured first, testing for the effect of increasing malate concentration (compare Malate alone, and pyruvate is added to compare FAO as the background state with CI&FAO as the reference state.

Abbreviation: PMOct

Reference: MiPNet21.06 SUIT reference assay





MitoPedia O2k and high-resolution respirometry: "SUIT state" is not in the list (O2k hardware, DatLab, Oroboros QM, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry) of allowed values for the "MitoPedia O2k and high-resolution respirometry" property. SUIT state"SUIT state" is not in the list (O2k hardware, DatLab, Oroboros QM, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry) of allowed values for the "MitoPedia O2k and high-resolution respirometry" property. 



PMOct(L)

PMOct(P)

  1. SUIT-RP2_4P: 1D 2Oct 3Mtit 3c (3NADH) 4P 5G 6S 7U 8Gp 9Rot 10Ama 11Tm 12Azd

PMOct(E)

  1. SUIT-RP1_4Oct: 1PM 2D 2c (2NADH) 3U 4Oct 5G 6S 7Rot 8Gp 9Ama 10Tm 11Azd

SUIT RP1


SUIT RP2