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== Publications: AmR == {{#ask:[[Additional label::AmR]]

|description=[[File:SUIT013 AmR ce D023.png|300px]] ...dependence in baker´s yeast. For more details see: [[Komlodi 2021 MitoFit AmR-O2]]. The respiration medium has to be choosen carefully since the [[Amplex

|abbr=AmR effect on ce - control |info='''B:''' Experimental control for [[SUIT-003 AmR ce D058]]

...:Publications]] [[Instrument and method::Oxygraph-2k]] [[Additional label::AmR]] ...ry:Abstracts]] [[Instrument and method::Oxygraph-2k]] [[Additional label::AmR]]

|description=[[File:SUIT013 AmR ce D023.png|400px]] |application=AmR

|abbr=AmR effect on ce ...trol in intact cells with oligomycin to study the inhibitory effect of the AmR assay components on the respiration ; ce: non-permeabilized cells -'''[[SUI

===SUIT-018 AmR mt D031=== [[File:SUIT-018 mt AmR D031 O2 DL-Files.png|600px]]

|application=AmR ...n be tested in separate protocols: [[SUIT-003 AmR ce D058]] and [[SUIT-003 AmR ce D059]]. For further information, ''see'': [[Amplex UltraRed]] page. To d

|application=AmR ..., see [[Setting the oxygen concentration]]. We recommend to run [[SUIT-018 AmR mt D040]] simultaneously at high oxygen concentration as a control.

...d cells - '''[[SUIT-009]]'''short protocol for H₂O₂ (AmR) for isolated mitochondria, tissue homogenate and permeabilized cells |application=AmR

|application=AmR [[File:SUIT-018 AmR mt D041-DL-File O2.png|600px]]

|abbr=CCP mt PM - AmR |application=AmR

|info='''B''': short protocol for H₂O₂ (AmR) in ce: non-permeabilized cells, pce: permeabilized cells -'''[[SUIT-009]] |O2k-Application: AmR

|application=AmR ...his protocol, we suggest not to go under ~100 µM O₂. [[SUIT-018 AmR mt D041]] has been designed to study O₂ dependence of H<sub>2</s

[[File:Komlodi 2021 MitoFit AmR-O2 graphical abstract.png|right|300px|Graphical abstract]] The fluorometric Amplex UltraRed AmR assay is frequently used for quantitative assessment of hydrogen peroxide p

...b>2</sub> measurements (AmR), apply [[SUIT-009 AmR mt D021]] or [[SUIT-009 AmR ce-pce D019]]. === SUIT-009 AmR mt D021 and SUIT-009 O2 mt D015 ===

|application=AmR SUIT-018 AmR mt D040 is a protocol to simultaneously study the oxygen dependence of O<s

...tract.png|right|300px|Graphical abstract]]The fluorometric Amplex UltraRed AmR assay is frequently used for quantitative assessment of hydrogen peroxide p ...(''1'') the medium specificity of the background fluorescence slope of the AmR assay, and (''2'') the oxygen dependence of H₂O₂ flux

:::* For H₂O₂-Application, choose [[SUIT-009 AmR ce-pce D019]]. ...Publications]] [[Additional label::SUIT-009 O2 ce-pce D016]] [[Additional label::O2k-Protocol]] [[Instrument and method::O2k-Protocol]]

...evealed contractile failure at nine but not 3 weeks after ischaemia whilst label-free quantitative proteomics identified an increase in proteins promoting a {{Template:Cited by Komlodi 2021 MitoFit AmR-O2}}

:::* For H₂O₂-Application, use [[SUIT-009 AmR mt D021]]. {{#ask:[[Category:Abstracts]] [[Additional label::SUIT-009 O2 mt D015]] [[Instrument and method::O2k-Protocol]]

.../sub> and H₂O₂ measurements (AmR), choose [[SUIT-006 AmR mt D048]], and for O₂ and membrane potential measurements, choos === SUIT-006 AmR mt D048 ===

{{#ask:[[Additional label::PLoSONE2022ace-sce]] {{Template:Cited by Komlodi 2021 MitoFit AmR}}

::::* [[SUIT-003 AmR ce D017]] this protocol has been designed to study the H₂O<sub>2 ...[[SUIT-003 AmR ce D058]] for studying the effect of the components of the AmR assay on the respiration using intact cells.

...ategory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Additional label::Leukemia]] |format=count}} | {{#ask:[[Category:Publications]] [[Additional label::Leukemia]] |format=count}}

{{#ask:[[Additional label::MitoFit 2021 CoQ]] {{Template:Cited by Komlodi 2021 MitoFit AmR}}

{{#ask:[[Category:Publications]] [[Additional label::SUIT-003 AmR ce D061]] [[Instrument and method::O2k-Protocol]]

{{#ask:[[Category:Publications]] [[Additional label::SUIT-003 AmR ce D060]] [[Instrument and method::O2k-Protocol]]

::: [[SUIT-006 AmR mt D048]] coupling control with pyruvate and malate as substrates for analy {{#ask:[[Category:Publications]] [[Additional label::SUIT-006 MgG mt D055]] [[Instrument and method::O2k-Protocol]]

::: [[SUIT-006 AmR mt D048]] coupling control with pyruvate and malate as substrates for analy {{#ask:[[Category:Publications]] [[Additional label::SUIT-006 MgG ce-pce D085]] [[Instrument and method::O2k-Protocol]]

...ntenance, calibrations and instrumental quality control for O₂, AmR, Fluo and MgG applications, without experimental sample in the incubation m ...abel, go to menu [Oroboros O2k]\O2k channel labels and set the new channel label as desired.

|abbr=AmR ...mitochondrial preparations. The reaction of H₂O₂ and AmR is catalyzed by [[horseradish peroxidase]] to produce the red fluorescent c