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Difference between revisions of "Template:Current week ecosystem agenda"

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{{Template:Science for Ukraine}}


  2020 Week 27
2024 Week 19


* '''Jun-29 Mo'''
* '''May-06 Mo'''
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]  
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|'''Is the noise seen in the slope of the oxygen signal (O2 slope neg.) within recommended values?'''<br>
|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
:An 'O2 slope neg.' within ± 2 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> is optimal (± 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> is acceptable).<br>
It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
- »[[Oxygen_calibration_-_DatLab#Instrumental_DL-Protocols| Air and zero calibration]]«
- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«  
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* '''Jun-30 Tu'''
* '''May-07 Tu'''  
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="110" align="center" | [[File:Tributes to pioneers icon.jpg|left|80px|BEC Tributes to pioneers in bioenergetics|link=Tributes to pioneers in bioenergetics]]
|'''The antidiabetic drug empagliflozin protects cardiac mitochondria against high-fat diet-induced dysfunction in mice'''<br>
|'''Tributes to pioneers in bioenergetics''' will provide a fresh look at concepts in a state of flow rather than as fixed paradigms, which tend to be forgotten or distorted without wandering in the garden of memories.<br>
:from the O2k-Network »[[LV Riga Makrecka-Kuka M]]«  
- »'''[[Tributes to pioneers in bioenergetics |Tributes to pioneers in bioenergetics - wandering in the garden of memories]]'''« - a new BEC Publication Series<br>
*Makrecka-Kuka M, Korzh S, Videja M, Vilks K, Cirule H, Kuka J, Dambrova M, Liepinsh E (2020) Empagliflozin protects cardiac mitochondrial fatty acid metabolism in a mouse model of diet-induced lipid overload. Cardiovasc Drugs Ther [Epub ahead of print]. [[Makrecka-Kuka 2020 Cardiovasc Drugs Ther|»Bioblast link«]]
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* '''Jul-01 We'''  
* '''May-08 We'''  
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* '''May-09 Th'''   
:{|
:{|
| width="100" align="center" |[[Image:TRANSMIT Acronym.jpg|70px|link=https://wiki.oroboros.at/index.php/TRANSMIT#TRANSMIT_dissemination |TRANSMIT]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''Kick-off of the TRANSMIT crowdfunding campaign: NOW'''<br>
|'''"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"''' <br>
:Bring the world of scientific research closer to the world of patients by supporting four cancer patients’ associations.
* Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem [[Vera-Vives 2024 Plant Physiol Biochem|»Bioblast link«]]
 
:from the O2k-Network »[[IT Padova Morosinotto T]
:- »[https://www.ideaginger.it/progetti/now.html https://www.ideaginger.it/progetti/now.html
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* '''Jul-02 Th'''  
* '''May-10 Fr'''
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'''<big>Next Week</big>'''
 
2024 Week 20
 
* '''May-13 Mo'''
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
| '''Did you know that if [[ET_capacity|ET capacity]] [[File:E.jpg]] is lower than [[OXPHOS_capacity|OXPHOS capacity]] [[File:P.jpg]] ([[ET_capacity#The_important_difference_between_states_P_and_E|''P''>''E'']]) in [[Living_cells | living cells]] or [[Mitochondrial_preparations | mitochondrial preparations]], then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:''' <br>
|'''Do you see fluctuations of O<sub>2</sub> slope negative following a pattern similar to the Peltier power, but without block temperature variation?'''<br>
- loss of oxidative capacity during the time course of the respirometric assay,<br>
Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O<sub>2</sub> slope negative.
- insufficient [[Uncoupler|uncoupler]] concentrations,<br>  
<br>
- over titration of an uncoupler, which inhibits ET capacity,<br>
- »'''[[Oxygen_flux_-_instrumental_background#Fluctuations_of_O2_slope_negative|Fluctuations of O<sub>2</sub> slope negative]]'''«<br>
- inhibition of ET capacity due to the use of high [[Oligomycin|oligomycin]] (inhibitor of the ATP synthase) concentrations employed to measure [[LEAK_respiration|LEAK respiration]] prior ET capacity.
- communicated by »[[Cardoso Luiza]]« and »[[Grings Mateus]]«
 
Information from: Gnaiger Erich ''et al'' ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]«  
|}
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* '''Jul-03 Fr'''
* '''May-14 Tu'''
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* '''May-15 We'''
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* '''May-16 Th'''  
:{|
:{|
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''Mitochondrial H<sub>2</sub>O<sub>2</sub> flux in cardiac and skeletal muscle preparations is influenced by experimental oxygen concentration''' <br>
|''' "The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."'''<br>
: from the O2k-Network »[[US CO Fort Collins Chicco AJ]]« <br>
- »'''[[MiPNet27.07 Sample Holder]]'''«<br>
*          Li Puma LC, Hedges M, Heckman JM, Mathias AB, Engstrom MR, Brown AB, Chicco AJ (2020) Experimental oxygen concentration influences rates of mitochondrial hydrogen peroxide release from cardiac and skeletal muscle preparations. Am J Physiol Regul Integr Comp Physiol 318:R972-80. [[Li Puma 2020 Am J Physiol Regul Integr Comp Physiol|»Bioblast link«]]
- communicated by »[[Willis Jaime R]]« and »[[Timon-Gomez Alba]]«
|}
|}
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* '''May-17 Fr'''
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Latest revision as of 16:15, 6 May 2024

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2024 Week 19
  • May-06 Mo
MitoPedia
What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010« 
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba« 


  • May-07 Tu
BEC Tributes to pioneers in bioenergetics
Tributes to pioneers in bioenergetics will provide a fresh look at concepts in a state of flow rather than as fixed paradigms, which tend to be forgotten or distorted without wandering in the garden of memories.

- »Tributes to pioneers in bioenergetics - wandering in the garden of memories« - a new BEC Publication Series


  • May-08 We

  • May-09 Th
O2k-Publications in the MiPMap "The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"
  • Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem »Bioblast link«
from the O2k-Network »IT Padova Morosinotto T«

  • May-10 Fr

Next Week

2024 Week 20
  • May-13 Mo
O2k-Open SupportInfo: take care Do you see fluctuations of O2 slope negative following a pattern similar to the Peltier power, but without block temperature variation?

Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O2 slope negative.
- »Fluctuations of O2 slope negative«
- communicated by »Cardoso Luiza« and »Grings Mateus«


  • May-14 Tu

  • May-15 We

  • May-16 Th
O2k-Open SupportInfo: improve "The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."

- »MiPNet27.07 Sample Holder«
- communicated by »Willis Jaime R« and »Timon-Gomez Alba«


  • May-17 Fr