Difference between revisions of "Thomas 2011 Mitochondrion"
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|injuries=Oxidative stress;RONS | |||
|organism=Human, Mouse | |organism=Human, Mouse | ||
|tissues=Heart, Skeletal muscle, Nervous system | |tissues=Heart, Skeletal muscle, Nervous system, Fibroblast | ||
|preparations=Homogenate, Isolated mitochondria | |preparations=Homogenate, Isolated mitochondria | ||
|topics=ATP, ATP production | |topics=ATP, ATP production | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
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Latest revision as of 15:36, 9 November 2016
Thomas RR, Khan SM, Portell FR, Smigrodzki RM, Bennett JP Jr (2011) Recombinant human mitochondrial transcription factor A stimulates mitochondrial biogenesis and ATP synthesis, improves motor function after MPTP, reduces oxidative stress and increases survival after endotoxin. Mitochondrion 11:108-18. |
Thomas RR, Khan SM, Portell FR, Smigrodzki RM, Bennett JP (2011) Mitochondrion
Abstract: Recombinant human mitochondrial transcription factor A protein (rhTFAM) was evaluated for its acute effects on cultured cells and chronic effects in mice. Fibroblasts incubated with rhTFAM acutely increased respiration in a chloramphenicol-sensitive manner. SH-SY5Y cells showed rhTFAM concentration-dependent reduction of methylpyridinium (MPP+)-induced oxidative stress and increases in lowered ATP levels and viability. Mice treated with weekly i.v. rhTFAM showed increased mitochondrial gene copy number, Complex I protein levels and ATP production rates; oxidative damage to proteins was decreased ~50%. rhTFAM treatment improved motor recovery rate after treatment with MPTP and dose-dependently improved survival in the lipopolysaccharide model of endotoxin sepsis. β’ Keywords: Recombinant TFAM, Mitochondrial biogenesis, ATP synthesis, MPTP, Sepsis
β’ O2k-Network Lab: US VA Richmond Bennett JP, US VA Richmond Iyer S
Labels:
Stress:Oxidative stress;RONS Organism: Human, Mouse Tissue;cell: Heart, Skeletal muscle, Nervous system, Fibroblast Preparation: Homogenate, Isolated mitochondria
Regulation: ATP, ATP production
HRR: Oxygraph-2k