Difference between revisions of "WG1 MitoEAGLE protocols, terminology, documentation"
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::::: '''[[Vendelin M|Marko Vendelin]]''', Dr. - [mailto:[email protected] [email protected]] | ::::: '''[[Vendelin M|Marko Vendelin]]''', Dr. - [mailto:[email protected] [email protected]] | ||
Revision as of 18:06, 4 January 2018
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News and Events |  |
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Working Groups | Short-Term Scientific Missions | Management Committee | Members |  |
COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
WG1 MitoEAGLE protocols, terminology, documentation
WG1 Action
- Standard operating procedures and user requirement document: Protocols, terminology, documentation
- Many analyses lack statistical power within a single study. Statistical combination of different studies is often difficult due to lack of methodological standardization and harmonization.
WG1 Action
WG Leader
- Kathrin Renner-Sattler, Mag. Dr. - [email protected]
WG Co-Leader
- Marko Vendelin, Dr. - [email protected]
Participants and discussion
Task groups
- TG leaders: suggestions discussed at the MitoFit Science Camp 2016 Kuehtai AT, and updated according to the MitoEAGLE MC Meeting 2016 Brussels BE.
- TG participants: please send us an Email to join: [email protected]
SUIT protocols and terminology
Task group 1.1
- Coupling states and coupling control ratios: consensus statements
- TG leaders: Renner-Sattler Kathrin DE / Gnaiger Erich AT / Hoppel Charles L US
- » The protonmotive force and respiratory control: Building blocks of mitochondrial physiology - Manuscript in preparation by the MitoEAGLE Working Group.
Task group 1.2
- SUIT protocols: nomenclature consistent with concept of database
- TG leader: Doerrier Velasco Carolina A AT, Gnaiger Erich AT
- TG participants: Attimonelli Marcella IT, Krako Nina RS, Vendelin Marko EE
Task group 1.3
- Experimental details in SUIT
- TG leaders: Chicco Adam J US/ Hickey Anthony J NZ/ Doerrier Velasco Carolina A AT
- TG participants: Casado Pinna Marta ES, Endlicher Rene CZ, Krako Nina RS, Kucera Otto CZ, Sobotka Ondrej CZ, Stankova Pavla CZ
- » MitoPedia: Sample preparations
- References
- Gnaiger E (2017) Quality first: a paradigm shift in mt-respiratory control towards global connection. Abstract MitoEAGLE Barcelona 2017. »Bioblast link«
- Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840, DOI:10.1038/s41598-017-02789-8. - »Bioblast link«
- References
Instrumental platforms: comparisons
Task group 1.4
- Instrumental platforms: comparisons
- TG leaders: Molina Anthony JA US
- TG participants: Canto Carles CH (suggested), Genova Maria Luisa IT, Tronstad Karl Johan NO (suggested), Vendelin Marko EE, Zorzano Antonio ES (suggested)
Knowledge management system and proficiency test
Task group 1.5
- Knowledge management system
- TG leaders: Drinnan Michael UK
- TG participants: Vendelin Marko EE, Garcia-Roves Pablo ES
- Towards and beyond a mitochondrial physiology database: filter and amplify more signals from this data, making them more meaningful and valuable for research and medicine.
Task group 1.6
- Proficiency test
- TG leaders: Gnaiger Erich AT/ Doerrier Velasco Carolina A AT
- TG participants: Genova Maria Luisa IT
- References
Documentation
Task group 1.7
- Documentation
- TG leaders: Arnould Thierry BE / Votion Dominique-Marie BE
- TG participants: Gnaiger Erich AT
- Preprints for MitoEAGLE
- Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of oxidative phosphorylation by temperature in the heart. bioRxiv doi10.1101/103457. - »Bioblast link«
- Preprints for MitoEAGLE
- Cores of reproducibility, data availability
- » Wagner P (2017) Cores of reproducibility in physiology (CORP): Advancing the corpus of physiological knowledge. J Appl Physiol 122:89-90.
- » Curran-Everett D (2017) CORP: Minimizing the chances of false positives and false negatives. J Appl Physiol 122:91-5.
- Think less about a simple P value and more about the scientific importance of the confidence interval bounds for some experimental result. Even a convincing P value of 0.005 can be associated with a confidence interval whose bounds indicate an effect that is scientifically inconsequential.
- Rely on repeated studies to accumulate evidence for some biological phenomenon.
- » Data availability statements in PLOS journals
- Cores of reproducibility, data availability
Dissemination and training
Task group 1.8
- Translation to community in general
- TG leader: Makrecka-Kuka Marina LV (Dissemination Coordinator), Rattan Suresh DK
- TG participants: Krako Nina RS, Irving Brian A US
Task group 1.9
- Dissemination Scientific community
- TG leaders: Makrecka-Kuka Marina LV / Gnaiger Erich AT
- TG participants: Ali Sameh S EG, Cervinkova Zuzana CZ, Chicco Adam J US, Coen Paul M US, Eide Lars NO, Garcia-Roves Pablo M ES, Gasparre Giuseppe IT, Genova Maria Luisa IT, Haendeler Judith DE, Iyer Shilpa US, Krako Nina RS, Laner Verena AT, Mayan Maria D ES, Mishmar Dan IL, Nehlin Jan DK, Palmeira Carlos PT, Paterlini Serena IT, Porcelli Anna Maria IT, Rao Raj US, Schaefer Patrick DE, Tronstad Karl Johan NO, Trougakos Ioannis GR, Volani Chiara AT, Votion Dominique-Marie BE
Task group 1.10
- Training, Short Term Scientific Missions
- TG leader: Labieniec-Watala Magdalena PL, Klaus Susanne DE
- TG participants: Ali Sameh S EG, Cervinkova Zuzana CZ, Chicco Adam J US, Coen Paul M US, Eide Lars NO, Garcia-Roves Pablo M ES, Gasparre Giuseppe IT, Genova Maria Luisa IT, Haendeler Judith DE, Iyer Shilpa US, Krako Nina RS, Mayan Maria D ES, Mishmar Dan IL, Nehlin Jan DK, Palmeira Carlos PT, Paterlini Serena IT, Porcelli Anna Maria IT, Rao Raj US, Schaefer Patrick DE, Tronstad Karl Johan NO, Trougakos Ioannis GR, Volani Chiara AT, Votion Dominique-Marie BE
Progress
- » 2017-11 MitoEAGLE Hradec Kralove 2017 CZ
- » 2017-07 MiPschool Obergurgl 2017 AT
- » 2017-03 MitoEAGLE Barcelona 2017 ES
- » 2016-11 MitoEAGLE Verona 2016 IT
Next steps
References
- General requirements for the competence of testing and calibration laboratories » ISO/IEC 17025:2005
