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WG1 MitoEAGLE protocols, terminology, documentation

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Revision as of 22:55, 28 January 2017 by Gnaiger Erich (talk | contribs)


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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping


WG1 MitoEAGLE protocols, terminology, documentation



WG1 Action

Standard operating procedures and user requirement document: Protocols, terminology, documentation

» WG1 Project application
MITOEAGLE Working Groups
Many analyses lack statistical power within a single study. Statistical combination of different studies is often difficult due to lack of methodological standardization and harmonization.


WG Leader

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Kathrin Renner-Sattler, Mag. Dr. - [email protected]





WG Co-Leader

Marko Vendelin, Dr. - [email protected]

Participants and discussion

» Talk:WG1 MITOEAGLE protocols, terminology, documentation


Track record

» 2017-07 MiPschool Obergurgl 2017
» 2017-03 MITOEAGLE 2017 Barcelona ES
» 2016-11 MITOEAGLE 2016 Verona IT


Task groups

TG leaders: suggestions discussed at the MitoFit Science Camp 2016 Kuehtai AT, and updated according to the MITOEAGLE MC Meeting 2016 Brussels BE.
TG participants: summary of the feedback received.

TG1.1 Coupling states and coupling control ratios: consensus statements

TG leaders: Renner-Sattler Kathrin DE / Gnaiger Erich AT / Hoppel Charles L US
TG participants:

TG1.2 SUIT protocols: nomenclature consistent with concept of database

TG leader: Doerrier Velasco Carolina A AT, Gnaiger Erich AT
TG participants: Attimonelli Marcella IT, Krako Nina RS, Vendelin Marko EE
» MiPNet21.06 SUIT reference protocol

TG1.3 Experimental details in SUIT

TG leaders: Chicco Adam J US/ Hickey Anthony J NZ/ Doerrier Velasco Carolina A AT
TG participants: Casado Pinna Marta ES, Endlicher Rene CZ, Krako Nina RS, Kucera Otto CZ, Sobotka Ondrej CZ, Stankova Pavla CZ
» MitoPedia: Sample preparations
» MitoPedia: Media for respirometry
» MitoPedia: Substrates and metabolites

TG1.4 Instrumental platforms: comparisons

TG leaders: Molina Anthony JA US
TG participants: Canto Carles CH (suggested), Genova Maria Luisa IT, Tronstad Karl Johan NO (suggested), Vendelin Marko EE, Zorzano Antonio ES (suggested)

TG1.5 Knowledge management system

TG leaders: Drinnan Michael UK
TG participants: Vendelin Marko EE, Garcia-Roves Pablo ES
  • Towards and beyond a mitochondrial physiology database: filter and amplify more signals from this data, making them more meaningful and valuable for research and medicine.
» MitoFit-KMP
» MitoFit Quality Control System
» MiPMap

TG1.6 Proficiency test

TG leaders: Gnaiger Erich AT/ Doerrier Velasco Carolina A AT
TG participants: Genova Maria Luisa IT
» MitoFit proficiency test
» MiPNet21.07 MitoFit DatLab PT

TG1.7 Documentation

TG leaders: Arnould Thierry BE / Votion Dominique-Marie BE
TG participants: Gnaiger Erich AT
Preprints for mitochondrial physiology
  1. The preprint server for biology: http://biorxiv.org/
  2. Vale RD (2015) Accelerating scientific publication in biology. Proc Natl Acad Sci U S A 112:13439-46. - »Bioblast link
  3. Berg JM et al (2016) Preprints for the life sciences. Science 352:899-901. - »Bioblast link - Ginsparg was emphatic that a preprint, because it has a time stamp and is publicly available, plays a key role in establishing priority of discovery.
Preprint relevant for WG1: protocols and terminology
  1. Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of oxidative phosphorylation by temperature in the heart. bioRxiv doi: https://doi.org/10.1101/103457.
Data availability
  1. Data availability statements in PLOS Biology

TG1.8 Translation to community in general

TG leader: Makrecka-Kuka Marina LV (Dissemination Coordinator), Rattan Suresh DK
TG participants: Krako Nina RS, Irving Brian A US

TG1.9 Dissemination Scientific community

TG leaders: Makrecka-Kuka Marina LV / Gnaiger Erich AT
TG participants: Ali Sameh S EG, Cervinkova Zuzana CZ, Chicco Adam J US, Coen Paul M US, Eide Lars NO, Garcia-Roves Pablo M ES, Gasparre Giuseppe IT, Genova Maria Luisa IT, Haendeler Judith DE, Iyer Shilpa US, Krako Nina RS, Laner Verena AT, Mayan Maria D ES, Mishmar Dan IL, Nehlin Jan DK, Palmeira Carlos PT, Paterlini Serena IT, Porcelli Anna Maria IT, Rao Raj US, Schaefer Patrick DE, Tronstad Karl Johan NO, Trougakos Ioannis GR, Volani Chiara AT, Votion Dominique-Marie BE

TG1.10 Training, Short Term Scientific Missions

TG leader: Labieniec-Watala Magdalena PL, Klaus Susanne DE
TG participants: Ali Sameh S EG, Cervinkova Zuzana CZ, Chicco Adam J US, Coen Paul M US, Eide Lars NO, Garcia-Roves Pablo M ES, Gasparre Giuseppe IT, Genova Maria Luisa IT, Haendeler Judith DE, Iyer Shilpa US, Krako Nina RS, Mayan Maria D ES, Mishmar Dan IL, Nehlin Jan DK, Palmeira Carlos PT, Paterlini Serena IT, Porcelli Anna Maria IT, Rao Raj US, Schaefer Patrick DE, Tronstad Karl Johan NO, Trougakos Ioannis GR, Volani Chiara AT, Votion Dominique-Marie BE

References

» ISO/IEC 17043:2010 General requirements for proficiency testing
» ISO 13528:2015 Statistical methods for use in proficiency testing by interlaboratory comparison
» ISO 15189:2012 Medical laboratories — Particular requirements for quality and competence
» ISO 17511:2003 In vitro diagnostic medical devices
» ISO 9001:2015 Quality management systems - requirements
» ISO 10012:2003 Measurement management systems
General requirements for the competence of testing and calibration laboratories » ISO/IEC 17025:2005


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