Hyderabad IN, 2011 Dec 08. Oroboros O2k-Workshop on high-resolution respirometry (HRR) - Satellite to 1st SMRM, IOC64. »IN Hyderabad Thangaraj K.
Abstract: International Course on High-Resolution Respirometry - Satellite to 1st SMRM. Hyderabad, India; 2011 December 08
ORO, IOC, 2011, O2k-Network
The 64th Workshop on High-Resolution Respirometry (HRR) is the First Oroboros O2k Workshop held in India. Participants are scientists, mostly the Ph.D. students, who want to learn in detail the experimental basis of high-resolution approaches to measure mitochondrial respiratory function. The workshop includes a basic introduction to the Oroboros O2k with integrated on-line analysis by DatLab 4.3 and applications of the Titration-Injection microPump TIP2k. Specific emphasis is placed on on-line practical demonstration of instrument and software performance, to introduce the participants into the technical and operational details of high-resolution respirometry (HRR). Quality control of instrumental performance will be demonstrated by on-line calibration of the oxygen sensors, evaluation of signal stability, and perspectives of HRR in mitochondrial physiology.
Novel developments are presented on substrate-uncoupler-inhibitor titration (SUIT) protocols. An overview is given on the O2k-MultiSensor System, with the TPP+ electrode for simultaneous measurement of mt-membrane potential and oxygen consumption. As an outlook to Oroboros innovations, our newest perspectives will be presented on the O2k-Fluorescence Module, which is developed in the frame of the project MitoCom Tyrol. Short presentations of 'Hot topics in Mitochondrial Physiology' extend the discussions on respiratory protocols.
O2k-Workshops are listed as MitoGlobal Events.