O2k-MultiSensor

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Oroboros Instruments

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O2k-MultiSensor

O2k-Catalogue

Description When one (or more) analytical parameters are monitored simultaneously with oxygen concentration and oxygen flux in the Oroboros O2k, this is an O2k-MultiSensor application. The O2k-FluoRespirometer fully supports the O2k-MultiSensor Modules. For some O2k-MultiSensor applications it is necessary to introduce one or more additional sensors into the chamber through a MultiSensor stopper. Optical applications require the standard black stoppers.
Product ID 3####-##
Type
Link ISE Manual MiPNet15.03
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O2k-Catalogue: O2k-MultiSensor

 DescriptionProduct idProduct image
O2k-Fluorometer Series GO2k-Fluorometer Series G (up to 2017-July) - the experimental system complete for high-resolution respirometry (HRR) combined with fluorometry. The O2k-Fluorometer includes the O2k-Core, O2k-Fluo LED2-Module and TIP2k, and supports all other add-on O2k-Modules of the Oroboros O2k. The O2k is a sole source apparatus with no other instruments meeting its specifications.10010-01
O2k-Fluorometer Series G
O2k-Fluo LED2-ModuleThe O2k-Fluo LED2-Module is a component of the O2k-Fluorometer (O2k-Series D to G). It is an amperometric add-on module to the O2k-Core (O2k-Series D to G), adding a new dimension to high-resolution respirometry. Optical sensors are inserted through the front window of the O2k-glass chambers, for measurement of hydrogen peroxide production (Amplex UltraRed), ATP production (Magnesium green), mt-membrane potential (Safranin, TMRM), Ca2+ (Calcium green), and numerous other applications open for O2k-user innovation. The O2k-Fluo LED2-Module consists of optical sensors for both O2k-Chambers (LEDs for green and blue excitation), optical filters and the Fluorescence-Control Unit for regulation of light intensity and data input into the O2k-Main Unit (O2k-Series D to G). It includes the updated DatLab software.12100-02
Fluorescence-Control Unit lettered.jpg
O2k-TPP+ ISE-ModuleO2k-TPP+ ISE-Module: 2 potentiometric ion-selective electrodes (ISE), ISE-Service Kit and 2 PEEK-Stoppers, supported by the Oroboros O2k (O2k-Series D and higher). It also includes the newest DatLab Version. The ISE module is designed with replaceable membranes making it possible to measure different ions such as TPP+ or Ca2+ with the same electrode housing. The main application of the O2k-TPP+ ISE-Module is measurement of mitochondrial membrane potential with tetraphenylphosphonium (TPP+) or TPMP+.12300-03
TPP new.jpg
O2k-pH ISE-ModuleO2k-pH ISE-Module: two pH electrodes and reference electrodes and accessories, two black PEEK stoppers, supported by the Oroboros O2k. It also includes the newest DatLab version. Quality control: Stability of Oroboros pH electrodes is tested for drift <15 µpH·s-1·mL-1.12400-02
PH new.jpg
O2k-NO Amp-ModuleO2k-NO Amp-Module: 2 black PEEK stoppers for amperometric O2k-MultiSensor application (NO, H2S, H2O2); 1 additional hole (2.3 mm); central titration port; conical bottom; with Volume-Calibration Rings (A and B); 2 O-rings mounted on each stopper, with 16 spare O-rings (O-ring\Viton\12.5x1 mm; two boxes). The NO sensor is not included.12500-02
O2k-NO Amp-Module.jpg
Stirrer-Bar\black PEEK\15x6 mmStirrer-Bar\black PEEK\15x6 mm, for specific applications with light guides to minimize optical interference.23220-01
Stirrer-Bar black PEEK15x6 mm.JPG
Stopper\black PEEK\conical Shaft\central PortStopper\black PEEK\conical Shaft\central Port: with conical shaft and one central capillary (with PTFE, graphite, carbon fiber), Volume-Calibration Ring (A or B) for volume adjustment 1.5 to 3.2 ml; 2 mounted O-rings, with 8 spare O-rings (O-ring\Viton\12.5x1 mm). The black PEEK stoppers are required for optical O2k-MultiSensor Modules (O2k-Fluo_LED2-Module). Two units of this item are standard components of the O2k-Assembly Kit.24000-01
Stopper black PEEK conical Shaft central Port.JPG
O-ring\Viton\12.5x1 mmO-ring\Viton\12.5x1 mm, for PVDF or PEEK O2k-Stoppers, box of 8 as spares. Two spare boxes of this product are standard components of the O2k-Assembly Kit (O2k-FluoRespirometer) as well as the O2k-TPP+ ISE-Module, O2k-pH ISE-Module and the O2k-NO Amp-Module.24310-02
O-ringViton12.5x1 mm.jpg
Cover-Slip\blackA Cover-Slip should be placed on top of the O2k-Stopper to minimize contamination and evaporation of liquid extruding from the capillary of the stopper. The Cover-Slips do not exert any direct effect on oxygen backdiffusion into the O2k-Chamber. Use the the Cover-Slip\black to avoid light penetration and disturbance of fluorescence signals and generally for optical measurements in the O2k. Two units of this item are standard components of the O2k-Assembly Kit.24411-01
Cover-Slip black.JPG
Forceps for membrane applicationForceps for membrane application: for OroboPOS and ISE membrane application; do not use for tissue preparation. This item is a standard component of the OroboPOS-Service Kit and the O2k-TPP+ ISE-Module.26700-01
Forcep for membrane application.jpg
O2k-Catalogue: O2k-Modules4#### O2k-Catalogue: O2k-Modules. O2k-Modules can be obtained with the O2k or added later, and can be simply installed by the user.4####4####
MultiSensor-ConnectorMultiSensor-Connector: for separate reference electrode and ISE; only for O2k-Series B and Series C with MultiSensor electronic upgrading before 2011.40420-00
30420-24 MultiSensorConnector.JPG
MultiSensor-Preamplifier 1/100MultiSensor-Preamplifier 1/100: Required only for O2k-Series A-C, for application of NO (or other amperometric) sensors (single chamber mode of application).40430-00
30430--24 NO-Attachment.JPG
O2k-Catalogue: O2k-MultiSensor\General41### O2k-Catalogue: O2k-MultiSensor\General41###41###
Stopper-Shaft angular\white PVDF\side PortStopper-Shaft angular\white PVDF\side Port: angular base, for drilling additional custom-made ports for user-specified O2k-MultiSensor applications.41111-00
Stopper\black PEEK\conical Shaft\central+side 2.5 mm PortStopper\black PEEK\conical Shaft\central+side 2.5 mm Port, black PEEK (with PTFE, graphite, carbon fiber); 1 additional hole (2.5 mm); central titration port; conical bottom; including Volume-Calibration Ring (A or B); 2 mounted O-rings (O-ring\Viton\12x1 mm).41220-01
Stopper\white PVDF\angular Shaft\side+5 mm PortStopper\white PVDF\angular Shaft\side+5 mm Port: 1 additional hole (5 mm); side titration port; angular bottom; including Volume-Calibration Ring (A or B); 2 mounted O-rings (O-ring\Viton\12x1 mm).41252-01
Stopper\white PVDF\angual Shaft\side+6 mm PortStopper\white PVDF\angual Shaft\side+6 mm Port: 1 additional hole (6 mm); side titration port; angular bottom; including Volume-Calibration Ring (A or B); 2 mounted O-rings (O-ring\Viton\12x1 mm).41262-01
Stopper\white PVDF\conical Shaft\central+specified PortsStopper\white PVDF\conical Shaft\central+specified Ports: for O2k-MultiSensor applications with user-specific sensors; with additional holes custom-made, according to customer specification (max. 3.5 mm diameter); including Volume-Calibration Ring (A or B); 2 mounted O-rings (O-ring\Viton\12x1 mm).41312-01
Stopper\white PVDF\angular Shaft\side+specified PortStopper\white PVDF\angular Shaft\side+specified Port: with additional holes custom-made according to customer specification (max. 8 mm diameter); angular bottom; including Volume-Calibration Ring (A or B); 2 mounted O-rings (O-ring\Viton\12x1 mm).41314-01
O2k-Catalogue: TPP+ ISE-Module42### O2k-Catalogue: TPP+ ISE-Module42###42###
ISE-Service BoxISE-Service Box: for storage and shipment of the O2k-TPP+ ISE-Module or O2k-pH ISE-Module.42001-01
ISE Package 1 TPP or CaO2k-TPP+ and Ca2+ ISE\1 Chamber: ISE-Package for 1 TPP+ and Ca2+ electrode.42010-01
ISE Package 1 TPP or Ca.JPG
Stopper\black PEEK\angular Shaft\side+6.2+2.6 mm PortStopper\black PEEK\angular Shaft\side+6.2+2.6 mm Port, for application with ISE; side titration port and two additional holes (6.2 mm and 2.6 mm); angular bottom; including Volume-Calibration Ring (A or B); 2 mounted O-rings, with 8 spare O-rings (O-ring\Viton\12.5x1 mm). Two stoppers are included in O2k-TPP+ ISE-Module.42100-01
Stopper black PEEK angular Shaft side+6.2+2.6 mm Port.JPG
Stopper-NeedleStopper-Needle: Short needle for bubble extrusion from port of the ISE-stopper.42120-01
Stopper-Needle.JPG
Ion-Selective Electrode TPP+ and Ca2+Ion-Selective Electrode TPP+ and Ca2+: ISE with 6 mm outer diameter shaft, for Stopper\white PVDF\angular Shaft\side+6.2+2.6 mm Port. O2k-TPP+ ISE-Module: 2 ISE.42200-01
Ion-Selective Electrode TPP+ and Ca2+.JPG
ISE-Inner Glass ElectrodeISE-Inner Glass Electrode of ISE, with Ag/AgCl- and Pt-wire42212-01
ISE-Inner Glass Electrode.JPG
ISE-Membrane SealISE-Membrane Seal for Ion-Selective Electrode TPP+ and Ca2+.42213-01
ISE-Membrane Seal.JPG
ISE-Compressible TubeISE-Compressible Tube for Ion-Selective Electrode TPP+ and Ca2+.42214-01
ISE-Compressible Tube.JPG
ISE-Consumables Spares SetISE-Consumables Spares Set42250-01
ISE-Consumables Spares Set.JPG
ISE-TPP+ MembranesISE-TPP+ Membranes, PVC, 4 mm diameter, box of 5 membranes. O2k-TPP+ ISE-Module: 2 boxes per electrode.42270-01
ISE-TPP+ Membranes.JPG
ISE-Ca2+ MembranesISE-Ca2+ Membranes: PVC, 4 mm diameter, box of 5 membranes. To be used with the O2k-TPP+ ISE-Module. The ISE-Ca2+ Membranes have to be ordered separately.42280-01
Ca2+ membranes.jpg
ISE-Membrane Mounting ToolISE-Membrane Mounting Tool for Ion-Selective Electrode TPP+ and Ca2+. O2k-TPP+ ISE-Module: mounting tool included.42300-01
ISA-Membrane Mounting Tool.JPG
ISE-Filling SyringeISE-Filling Syringe with needle for Ion-Selective Electrode TPP+ and Ca2+.42310-01
ISE-Filling Syringe.JPG
Reference-Electrode\2.4 mmReference-Electrode\2.4 mm: 2.4 mm diameter glass shaft, for ISE.42400-01
Reference Electrode 2.4 mm.jpg
Replacement-BarrelReplacement-Barrel for Reference-Electrode\2.4 mm, 2.4 mm diameter glass.42401-01
Replacement-Barrel.jpg
Electrolyte\Reference-ElectrodeElectrolyte\Reference-Electrode for Reference-Electrode\2.4 mm42402-01
Electrolyte Reference-Electrode.jpg
O2k-Catalogue: pH-Electrode43### O2k-Catalogue: pH-Electrode43###43###
PH-Glass electrodepH-Glass electrode with BNC plug. Two pH electrodes are included in the O2k-pH ISE-Module.43200-01
PH-Glass Electrode.JPG
O2k-Catalogue: O2k-Fluorescence44### O2k-Catalogue: O2k-Fluorescence44###44###
Fluorescence-Control UnitFluorescence-Control Unit with O2k-Front Fixation, Current-Control (O2k-Chamber A and B) for regulation of light intensity of the LED in the fluorescence sensors. This item is a standard component of the O2k-Fluorescence LED2-Module.44100-01
Fluorescence-Control Unit lettered.jpg
Fluorescence-SensorFluorescence-Sensor: LED with specified wavelength, photodiode, Filter-Cap attached with specific optical filter for the LED and/or photodiode. See Fluorescence-Sensor Green and Fluorescence-Sensor Blue.442##
Fluorescence-Sensor.jpg
Fluorescence-Sensor GreenFluorescence-Sensor Green: excitation LED 525 nm (dominant wavelength), photodiode, Filter-Cap equipped with Filter Set AmR for Amplex UltraRed measurements when delivered. Two units of this item are standard components of the O2k-Fluo LED2-Module.44210-01
Fluorescence-Sensor Green.JPG
Fluorescence-Sensor BlueFluorescence-Sensor Blue: excitation LED 465 nm (dominant wavelength), photodiode, Filter-Cap equipped with Filter Set Saf for measurement of mitochondrial membrane potential with Safranin when delivered. Filter sets for Magnesium green® / Calcium green® measurements are included. Two units of this item are standard components of the O2k-Fluo LED2-Module.44220-01
Fluorescence-Sensor Green.JPG
Filter-CapFilter-Cap: O2k-Fluo LED2-Module (O2k-Series D to G) sensors (Fluorescence-Sensor Green and Fluorescence-Sensor Blue) and O2k-FluoRespirometer (O2k-Series H) sensors (Smart Fluo-Sensor Green and Smart Fluo-Sensor Blue) are equipped with a removable Filter-Cap for exchange of optical filters for the optical pathways from the LED to the sample and from the sample to the photodiode.44310-01
Filter-Cap.JPG
Filter Set AmRFilter Set AmR: Set of filters for the determination of H2O2 production with Amplex UltraRed. These filters should be used together with Fluorescence-Sensor Green. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular). One pair of these filters is attached to Fluorescence-Sensor Green when delivered as part of the O2k-Fluorescence LED2-Module. One filter set is a standard component of the O2k-Fluorescence LED2-Module and O2k-FluoRespirometer.44321-01
Filter Set AmR.JPG

AmR excitation scan 1mA.png

Excitation spectrum obtained with the Fluorescence-Sensor Green at 1mA current supply and equipped with the Filter Set AmR.
Filter Set SafFilter set Saf: Set of filters for the (qualitative) determination of mitochondrial membrane potential with Safranin. These filters should be used together with Fluorescence-Sensor Blue. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular). One pair of these filters is attached to Fluorescence-Sensor Blue when delivered as part of the O2k-Fluorescence LED2-Module.


One filter set is a standard component of the O2k-Fluorescence LED2-Module and O2k-FluoRespirometer.
44322-01
Filter Set Saf.JPG

Saf excitation scan 1mA.png

Excitation spectrum obtained with the Fluorescence-Sensor Blue at 1mA current supply and equipped with the Filter Set Saf.
Filter Set MgG / CaGFilter set MgG / CaG: Set of filters for the determination of concentraions of Mg2+ or Ca2+ with the fluorophores Magnesium green and Calcium green, respectively. These filters should be used together with Fluorescence-Sensor Blue. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular). One filter set is a standard component of the O2k-Fluorescence LED2-Module and O2k-FluoRespirometer.44323-01
Filter Set MgG CaG.JPG

MgG excitation scan 1mA.png

Excitation spectrum obtained with the Fluorescence-Sensor Blue at 1mA current supply and equipped with the filter set for Magnesium green® / Calcium green®.
O2k-Catalogue: NO-Amp-Module46### O2k-Catalogue: NO-Amp-Module46###46###
Stopper\black PEEK\conical Shaft\central Port\peripheral 2.3 mm PortStopper\black PEEK\conical Shaft\central+peripheral 2.3 mm Port: for potentiometric O2k-MultiSensor applications (NO, H2S, H2O2); 1 additional port (2.3 mm); central titration port; conical bottom; including Volume-Calibration Ring (A or B); 2 mounted O-rings, with 8 spare O-rings (O-ring\Viton\12.5x1 mm). O2k-MultiSensor: two stoppers in O2k-NO Amp-Module.46100-01
Stopper black PEEK conical Shaft central Port peripheral 2.3 mm Port.JPG
... further results

O2k-MultiSensor Manual

ChapterSectionLast update
MiPNet15.03 O2k-MultiSensor-ISE
O2k-Manual
O2k-MultiSensor system with ion selective electrodes (ISE).
2018-01-25
MiPNet15.05 NO-manual
O2k-Manual
O2k-MultiSensor system with amperometric sensors: NO, H2S, H2O2.
2016-04-21
MiPNet17.05 O2k-Fluo LED2-Module
O2k-Manual
O2k-Fluo LED2-Module.
2016-08-09
MiPNet23.15 O2k-pH ISE-Module
O2k-Manual
Measurement of proton flux with the O2k-pH ISE-Module.
2018-09-08

See also: