Category:NextGen-O2k
The revolutionary all-in-one instrument to conquer mitochondrial disease
MitoPedia: NextGen-O2k
- » NextGen-O2k«
Term | Abbreviation | Description |
---|---|---|
Hydrogen peroxide | H2O2 | Hydrogen peroxide, H2O2 or dihydrogen dioxide, is one of several reactive oxygen intermediates generally referred to as reactive oxygen species (ROS). It is formed in various enzyme-catalyzed reactions (e.g., superoxide dismutase) with the potential to damage cellular molecules and structures. H2O2 is dismutated by catalase to water and oxygen. H2O2 is produced as a signaling molecule in aerobic metabolism and passes membranes more easily compared to other ROS. |
Mitochondrial membrane potential | mtMP, Δψ [V] | The mitochondrial membrane potential, mtMP, is the electric part of the protonmotive force, ΔpH+.
Δψ = ΔpH+ - ΔµH+ / F mtMP or Δψ is the potential difference across the inner mitochondrial (mt) membrane, expressed in the electric unit of volt [V]. Electric force of the mitochondrial membrane potential is the electric energy change per ‘motive’ electron or per electron moved across the transmembrane potential difference, with the number of ‘motive’ electrons expressed in the unit coulomb [C]. |
NextGen-O2k | ||
NextGen-O2k Instrument | NextGen-O2k Instrument | |
NextGen-O2k Technical developments | ||
Oxygen kinetics | Oxygen kinetics describes the dependence of respiration of isolated mitochondria or cells on oxygen partial pressure. Frequently, a strictly hyperbolic kinetics is observed, with two parameters, the oxygen pressure at half-maximum flux, p50, and maximum flux, Jmax. The p50 is in the range of 0.2 to 0.8 kPa for cytochrome c oxidase, isolated mitochondria and small cells, strongly dependent on Jmax and coupling state. | |
PB-Module | PB-Module | The PB-Module has been developed for conducting measurements of PhotoBiology, including photosynthesis. It consists of the PB-Sensor and electronic components which are an integral part of the NextGen-O2k. Measurements are recorded and evaluated with the DatLab 8 software. |
PB-Sensor | The PB-Sensor has been designed as a part of the PB-Module to provide with an external source of light, in the range of the blue (451 nm) or red (634 nm) wavelengths. This enables experiments for evaluating the production/consumption of O2 in the presence of light. The PB-Sensor consists on one LED and one photodiode mounted on the sensor tip behind a plastic cover. The LED emits with blue or red light on the sample and its intensity can be regulated from 0 to 5000 µEinsteins*m-2*s-1. The photodiode provides real-time measurement of the light intensity allowing for continuous adjustment to the desired value, which can also help compensate for the temperature rise due to the operation of the LED. | |
Q-Module | Q-Module | The Q-Module, developed both for measuring the Q redox state and for cyclic voltammetry measurements, is an integral part of the NextGen-O2k and consists of the Q-Sensor, integrated electronic components in the O2k, and the DatLab software. |
Q-Sensor | The Q-Sensor has been designed as a part of the Q-Module for measurements with cyclic voltammetry and voltammetry, allowing for analysis of the Q redox state. The Q-Stopper with the reference electrode is called Q-Sensor, which is plugged in the NextGen-O2k. A three-electrode system is used to detect the Q redox state. Two of the three electrodes (glassy carbon and platinum electrode) are built into the Q-Stopper, while the reference electrode is removable (Reference-Electrode\2.4 mm). |
- Bioblast links: Q - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coenzyme Q
- » Coenzyme Q
- » Quinone, Ubiquinone Q; oxidized
- » Quinol, Ubiquinol QH2; reduced
- » Semiquinone
- » Coenzyme Q2
- » Q-redox state
- » Q-pools
- Coenzyme Q
- Mitochondrial pathways, respiratory Complexes, and Q
- » Q-cycle
- » Q-junction
- » Convergent electron flow
- » NS-pathway
- » FNS
- » FNSGp
- Mitochondrial pathways, respiratory Complexes, and Q
- NextGen-O2k and Q-Module
- Bioblast links: NADH - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
Mitochondrial NADH and respiration
Mitochondrial NADH production
NextGen-O2k
- Bioblast links: Hydrogen peroxide - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Specific
- General
- MiPNet O2k-Procedures
- Bioblast links: ATP production - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
Phosphorylation pathway
Phosphorylation pathway substrates
- » ADP
- » ATP
- » Inorganic phosphate
Phosphorylation pathway inhibitors
Coupling control
Respiratory complexes and coupling
ATP production measurement
- Bioblast links: pH and protons - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- pH and protons
- » pH
- » hydrogen ion
- » proton
- » pH buffering capacity
- » proton flux
- » proton pump versus hydrogen ion pump
- » proton leak
- » proton slip
- » protonmotive force
- pH and protons
- O2k-pH
- » O2k-Catalogue: O2k-pH ISE-Module
- » O2k-Manual pH electrode: MiPNet23.15 O2k-pH ISE-Module
- » O2k-SOP: MiPNet08.16 pH calibration
- » File:PH-Calibration-List.xls
- » NextGen-O2k, ratiometric: Carboxy SNARF 1
- » NextGen-O2k, ratiometric: HPTS
- » pH calibration buffers
- O2k-pH
- O2k-Publications
- HRFR - general
- » O2k-Manual: MiPNet22.11 O2k-FluoRespirometer manual
- » O2k signals and output
- » O2k-SOP: MiPNet14.06 Instrumental O2 background
- » MiPNet19.18A O2k-Series G: Start
- » ESD
- » O2k configuration
- » O2k control
- » O2k-FluoRespirometer
- » O2k-Main Unit#O2k-Series
- » Titration-Injection microPump
- » Compare: O2k-TPP+_ISE-Module
- HRFR - general
- Bioblast links: Calcium - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Inhibitors
- General
- Bioblast links: PhotoBiology and plant physiology - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
PhotoBiology: photosynthesis
Plant physiology: respiration
NextGen-O2k and PB-Module
- » NextGen-O2k
- » PB-Module
- » PB-Sensor
Pages in category "NextGen-O2k"
The following 200 pages are in this category, out of 359 total.
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- Fatty acid oxidation
- FI Helsinki Jacobs HT
- Filter Set AmR
- Filter Set MgG / CaG
- Filter Set Saf
- Filter-Cap
- Fluorescence-Control Unit
- Fluorescence-Sensor Blue
- Fluorescence-Sensor Green
- Fluorescence-Service Box
- Forceps for membrane application
- Forceps\stainless Steel\angular Tip\fine
- Forceps\stainless Steel\rounded Tip\sharp
- Forceps\stainless Steel\straight Tip\sharp
G
H
I
- Iglesias-Gonzalez Javier
- Illumination
- Ion-Selective Electrode TPP+ and Ca2+
- ISE Package 1 TPP or Ca
- ISE-Ca2+ Membranes
- ISE-Compressible Tube
- ISE-Consumables Spares Set
- ISE-Filling Syringe
- ISE-Inner Glass Electrode
- ISE-Membrane Mounting Tool
- ISE-Membrane Seal
- ISE-Service Box
- ISE-TPP+ Membranes
- ISS-Filter and Tubing
- ISS-Integrated Suction System
- ISS-Integrated Suction System\120 V\US-CA
- ISS-Integrated Suction System\230 V\AU-NZ
- ISS-Integrated Suction System\230 V\EU
- ISS-Lid
- ISS-Steel Housing
- ISS-Waste Bottle
- IT Padova Viscomi C
M
- Magnesium Green
- Microbalance 120 g
- Microbalance 220 g
- Microbalance-Set
- Microbalance-Transport Case
- Microsyringe\10 mm3 51/0.13 mm
- Microsyringe\100 mm3 51/0.41 mm
- Microsyringe\200 mm3\TIP2k
- Microsyringe\25 mm3 51/0.15 mm
- Microsyringe\50 mm3 51/0.15 mm
- Microsyringe\500 mm3\TIP2k
- MiP2019/MitoEAGLE Belgrade RS
- MiPNet24.01 IOC139 Schroecken AT
- MiPNet24.02 IOC141 Schroecken AT
- MiPNet24.04 IOC140 Amsterdam NL
- MiPNet24.13 IOC142 Fukuoka JP
- MiPNet24.15 IOC143 Haryana IN
- MiPNet25.01 IOC152 Schroecken AT
- MiPNet25.02 IOC Schroecken AT
- MiPNet25.03 IOC144 Innsbruck AT
- MiPNet25.06 IOC145 Innsbruck AT
- Talk:MiPNet25.09 IOC San Diego CA US
- MiPNet25.10 Distributor engagement Tokyo JP
- MiPNet25.16 Virtual O2k-Workshop HRR
- MiPNet25.17 Virtual O2k-Workshop PhotoBiology
- MiPNet26.02 Virtual O2k-Workshop:Q-Module
- MiPschool Coimbra 2019
- MiR05-Kit
- Mitochondrial membrane potential
- MitoEAGLE WG2 Innsbruck AT
- MitoEAGLE WG4 Innsbruck AT
- MitoKit-CII
- MitoPedia: BEC
- MitoPedia: DatLab
- Talk:MitoPedia: Fluorometry
- MitoPedia: Gentle Science
- MitoPedia: O2k
- MitoPedia: O2k hardware
- MitoPedia: O2k-FluoRespirometry
- MitoPedia: O2k-Open Support
- MitoPedia: O2k-Respirometry
- MitoPedia: Oroboros QM
- MitoPedia: SUIT
- Molina Anthony JA
- Moore Anthony L
- Morosinotto Tomas
- Motic Microscope
- MultiSensor-Connector
- MultiSensor-Preamplifier 1/100
N
O
- O-ring sV\Viton\9.5x1 mm
- O-ring\Viton\12.5x1 mm
- O-ring\Viton\16x2 mm
- O-ring\Viton\18x2 mm
- O-ring\Viton\6x1 mm
- O-ring\Viton\8x1 mm
- O-ring\Viton\9x1 mm
- O2-Zero Powder
- O2k repair
- O2k series
- O2k-Amperometric OroboPOS Twin-Channel
- O2k-Assembly Kit
- O2k-Barometric Pressure Transducer
- O2k-Catalogue: Microbalance Mettler-Toledo
- O2k-chamber
- O2k-Chamber Holder
- O2k-Chamber Holder sV
- O2k-Chamber sV
- O2k-Dissection Set
- O2k-Electromagnetic Stirrer Twin-Control
- O2k-Events
- O2k-Fluo LED2-Module
- O2k-Fluo Smart-Module
- O2k-FluoRespirometer
- O2k-Fluorometer Series G
- O2k-Fuse Power Plug\M2.5 A\5x20 mm
- O2k-Main Basic
- O2k-Main Power Cable
- O2k-Main Power Cable\120 V\US-CA
- O2k-Main Power Cable\230 V\AU-NZ
- O2k-Main Power Cable\230 V\Europe
- O2k-Main Unit
- O2k-Manual
- O2k-MultiSensor
- O2k-NO Amp-Module
- O2k-Open Support
- O2k-Open Support agreement
- O2k-Peltier Mitopedia