Talk:MitoEAGLE protocols, terminology, documentation

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Participants

CountryCityContact
ArmeniaYerevanTrchounian K
AustraliaMelbourneStepto Nigel K
AustraliaDarlinghurst, SydneyStocker Roland
AustraliaSyndeyTon Riccardo
AustraliaBurwoodTrewin Adam J
AustraliaMelbourneHewakapuge Sudinna
AustraliaQueenslandDong Lan-Feng
AustraliaClayton VictoriaAlton Lesley
AustraliaGeelong, VictoriaMcKenzie Matthew
AustraliaMelbourneFerri Alessandra
AustraliaParkvilleHardee Justin P
AustraliaMelbourneLionett S
AustraliaQueenslandFisher Joshua J
AustraliaMelbourneCassar Samantha
AustraliaVictoriaVan Bergen Nicole J
AustraliaMelbourneKuang Jujiao
AustraliaSydneyKrycer James R
AustraliaMelbourneCoughlan Melinda T
AustraliaSydneyHoehn Kyle L
AustraliaVictoriaWinwood-Smith Hugh
AustraliaMelbourneGenders Amanda J
AustraliaMelobourneBotella Javier
AustraliaClaytonWolff Jonci Nikolai
AustraliaNedlandsFilipovska Aleksandra
AustraliaSouthportVidimce Josif
AustraliaQueenslandHolland Olivia J
AustraliaMelbourneWhitfield Jamie
AustraliaQueenslandRadenkovic Filip
AustraliaMelbourneSaner Nicholas
AustraliaMelbourne, VICMuccini Anna Maria
AustraliaNedlandsPerks Kara L
AustriaInnsbruckBergmeister Lisa
AustriaInnsbruckThapa Maheshwor
AustriaInnsbruckHoppel Florian
AustriaInnsbruckSchartner Melanie
AustriaInnsbruckDoerrier Carolina
AustriaSalzburgKofler B
AustriaInnsbruckCecatto Cristiane
AustriaInnsbruckKomlodi Timea
AustriaInnsbruckGallee Leon
AustriaInnsbruckDi Marcello Marco
AustriaInnsbruckNindl Veronika
AustriaInnsbruckBurtscher Martin
AustriaInnsbruckGarcia-Souza Luiz F
AustriaInnsbruckOroboros
AustriaInnsbruckGoebel G
AustriaInnsbruckPerez Valencia Juan Alberto
AustriaInnsbruckFischer Michael J
AustriaInnsbruckKarabatsiakis Alexander
AustriaInnsbruckLaner Verena
AustriaInnsbruckSchwarzer Christoph
AustriaInnsbruckHaider Markus
AustriaKlosterneuburgSazanov Leonid A
AustriaInnsbruckGnaiger Erich
AustriaInnsbruckKlocker Helmut
AustriaInnsbruckHuete-Ortega Maria
AustriaInnsbruckPassrugger Manuela
AustriaInnsbruckSchmitt Sabine
AustriaInnsbruckAntunes Diana
AustriaInnsbruckKeller Markus A
AustriaInnsbruckKapferer Werner
AustriaInnsbruckIglesias-Gonzalez Javier
AustriaViennaKozlov Andrey V
AustriaInnsbruckPlangger Mario
AustriaInnsbruckMeszaros Andras
AustriaInnsbruckCardoso Luiza HD
BelgiumBrusselsSonveaux P
BelgiumLeuven, FlandersBaekelandt V
BelgiumLiegeVotion Dominique-Marie
BelgiumNamurArnould Thierry
BelguimSart Tilman (Liège)Kruse C
BrasilPorto AlegreHaas Clarissa
BrasiliaCampinasRopelle Eduardo R
BrazilRio de JaneiroOliveira Marcus F
BrazilJaboticabalOliveira Marcos Tulio
BrazilCampinasVercesi Anibal E
BrazilRio de JaneiroGalina Antonio
BrazilSão PauloCrispim Marcell
BrazilSao PauloSilber Ariel Mariano
BrazilSão PauloKowaltowski Alicia J
BrazilSanta Maria - RSGoncalves Debora Farina
BrazilSanta Maria - Rio Grande do SulSoares Felix Alexandre Antunes
CanadaMontréalBreton Sophie
CanadaVancouver, BCTausan Daniel
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CanadaMontrealBettinazzi Stefano
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DenmarkCopenhagen NMaise Chroeis K
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DenmarkCopenhagenSoendergaard SD
DenmarkAarhus NJespersen Nichlas Riise
DenmarkAarhus NBoetker Hans Erik
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USACorvallisBatterson Philip M
USAAnn ArborTyrrell Daniel J
USABaton RougeHand Steven C
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USAAkronCohen Bruce H
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USALa JollaSchilling Jan M
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USALouisvilleMenze Michael A
USAGalvestonDurham William J
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USAAuroraFord Ellen
USACorvallisNewsom Sean A
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USAKansas CityThyfault John P
USAAuroraMoreau Kerrie
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USAIndianapolisBrozinick Joseph T
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USABlacksburgBrown David A
USAPortlandGarlid Keith D
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USADentonDzialowski Edward M
USABerkeleyWilliams Caroline M
USAFayettevilleRao RR
USAOklahoma CityAhn Bumsoo
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USABirminghamMoellering Douglas R
USANew YorkGalkin Alexander
USANashvilleWasserman David H
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USAStillwaterDavis Michael S
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USAClevelandHoppel Charles L
USAClevelandTandler Bernard
USAAtlantaJang Young Charles
USAPhiladelphiaGanetzky Rebecca
USAPuskarich Michael A
USASan Diego; La JollaMolina Anthony JA
USARochesterNair K Sreekumaran
USAProvoHancock Chad R
USAOrlandoCoen Paul M
USAOrlandoSparks Lauren M
USALittle RockAdams Sean H
USASan DiegoCannon Daniel T
USALittle RockMacMillan-Crow Lee Ann
USASan AntonioValentine Joseph Marco
USAOrlandoPino Maria F
USAPhiladelphiaOrynbayeva Zulfiya
USAGainesvilleLeeuwenburgh Christiaan
USAWinston-SalemGonzalez-Armenta Jenny L
USANorfolkLai Nicola
USANew HavenCavalcanti-de-Albuquerque Joao Paulo
USAMilwaukeeDash Ranjan K
USAFairbanksO'Brien Kristin
USABethesdaFessel Joshua Patrick
USAPhiladelphiaPiel Sarah
USARochesterLanza Ian R
USALouisvilleSkolik Robert A
USABethesdaSharma Pushpa
USABaltimoreGonzalez-Freire Marta
USAPhiladelphiaJang David H
USAMiamiFontanesi F
USAOrlandoGoodpaster Bret H
USALittle RockParajuli Nirmala
USAWakefieldVarricchio Frederick
USAFort CollinsLi Puma Lance C
USAKansas CitySwerdlow Russell H
USAFayettevilleIyer Shilpa
... further results


Comments and discussion

  1. We are mostly interested in two WG: (1) Since we are working mostly with heart and muscles, we are interested in WG 2 - MITOEAGLE data repository in muscle and other tissues. (2) WG 1 - Standard operating procedures and user requirement document: Protocols, terminology, documentation. Since we have an expertise in fatty acid metabolism, we are specially interested in experimental protocols for the evaluation of mitochondrial capacities regarding fatty acid metabolism. Of course our parts of the WG are also in our interest. - Marina Makrecka-Kuka, Edgars Liepinsh (2016).
  2. My role in Mito-EAGLE would be mainly in WG 2 and 4, with a focus on skeletal and cardiac muscle and cultured cardiomyocytes and endothelial cells. I would particularly like to bridge the gap between the group leaders and young investigators, trying to represent the young people who are the active users of the OROBOROS equipment. This will fit in nicely with the goals in WG1. ... Major congratulations on obtaining this beautiful collaborative grant! Very glad to see this network getting the recognition it deserves! Looking forward to being part of this! - Rob C Wuest (2016).
  3. Please, find enclosed my declaration to participate with my team in the following workgroups: WG1, WG2, WG3, WG4. We are now involved and have been in not a distant past – in studying of the following cell /tissue types:
    1. Blood cells, with particular emphasis on platelets and lymphocytes – isolated from animals and humans.
    2. Endothelial cells, fibroblasts, neurocytes (neuroblastoma), astrocytes, cancer cell lines – predominantly cultured.
    3. Hepatocytes, cardiomyocytes, cell isolated from brain – isolated from lab animals. - Cezary Watala (2016).
  4. I have already contacted the portuguese CNC to nominate me for the MC of theaction. Concerning my active participation, taking into account the type of work we do in my lab I could be included in all WGs. As you know we work with mitochondria from liver, muscle and fat, as well as cultured cells from different origins (WG2-4). The elaboration of SOPs (WG1) we can certainly contribute (see the book I edited on Mitochondrial Bioenergetics and in which you contribute a chapter). I believe that everybody can be part of the WG5, but as the coordinators of the action, you and Sandra should decide what is best for the success of the action. - Carlos Palmeira (2016).
  5. I would propose my participation in the WG1: Standard operating procedures and user requirement document: Protocols, terminology, documentation, considering that the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. I could give my active contribution in the phase of making platform that will aggregate the Action’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases in particular tasks:
    1. Develop reccomendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalised service for data management, data mining and health-care conforming standards of data interpretation
    2. Provide a summary on strategic dissemination and an education programme for MITOEAGLE.
    3. Considering that we are in process of buying the oxygraph, thus broading capacities of my lab, I would be happy to open the door of my new lab for the innovative inter-laboratory ring test experiments and together with my team contribute in that phase of the MITOEAGLE Action. I look forward to the establishment of MitoGlobal EAGLE network where, as stated in the MoU, researchers will collaborate on mapping mitochondrial physiology and medicine. The creation of such a unique well-coordinated network of senior researchers and young investigators will definitely put an added value to existing data and publications, will increase quality and validity of the research, will bring new possibilities of data sharing and data mining and will hopefully bring us to the era of more economical research by saving resources through standardised and harmnonised protocols, where the measuremnts will not be wasted due to lack of comparability. - Nebojsa Lalic (2016).
  6. Our research interest is oriented to reproductive endocrinology; especially to mechanisms that govern steroidogenesis of testicular Leydig cells in disturbed homeostasis. We use several in vivo models:
    1. stress -/+ systemic or intratesticular blockade of different stress receptors (alpha1ADR; betaADR; GR)
    2. hypogonadal-hypogonadism -/+ testosterone-replacement therapy
    3. androgens anabolics treatment -/+ blockade od AR
    4. aged rats -/+ Viagra-treatment
    5. biological clock - Silvana Andric (2016).
  7. I took a look to the MoU and the different working groups. You know well my research focus and having this into account I am interested in actions related to Working group 1 (Respirometric reference protocols, Interlaboratory proficiency test, Instrumental platforms). Working group 2 and 3: I have generated multiple mitochondrial respiration data sets in different mouse tissues (mainly in skeletal muscle, hypothalamus, liver and white adipose tissue). Currently we are also performing these assays in white adipose tissue from human patients. Hence I am interested in all milestones of WG 2 and 3. WG 5 is related to WG 1 and as you know I have interest in training, dissemination and also together with my collaborators (if needed) we could help with data sets and a data interpretation. Overall, I have a general interest on this Action and my active role in MITOEAGLE could be discussed. I could be of help participating in some of the tasks where my expertise and interest fit best. We will be forming and interdisciplinary research group with four group leaders: JC Perales, R Guimera, M Sales-Pardo and PM Garcia-Roves with two different affiliations, University of Barcelona (JCP and PMG-R - Energy metabolism) and University Rovira I Virgili (RG and MS – Data base, statistics, data mining). - Pablo M Garcia-Roves (2016).
  8. I will be pleased to contribute to the tasks of WG1 by developing interlaboratory proficiency tests and instrumental platform comparison. Moreover, as a (potential) MC member of MitoEAGLE I can also offer visibility to the Action itself (i.e. WG5: MITOEAGLE dissemination and training, according to mitoglobal.org webpages) by establishing new interactions with the International Coenzyme Q10 Association (ICQA) where I act as a member of the Executive Committee. ICQA organizes biennal conferences on new highlights of Coenzyme Q research. In 2015, we held the conference in Bologna (http://conference2015.wix.com/icqaconference) and we included in the program also some scientific sessions dedicated to mitochondrial bioenergetics and diseases. I will be pleased to enforce cooperation between ICQA and MitoEAGLE. - Maria Luisa Genova (2016).
  9. We found the scientific content and objectives of the call very interesting and compatible with our wide expertise on oxysterol analysis in food and biological systems. Our group has a large experience on lipid composition and their degradation products (volatile and non-volatile), as well as on sensory analysis. Moreover, we are currently involved in the H2020-GERI-2014-1 project "PLOTINA" ("Promoting gender balance and inclusion in research, innovation and training") with Prof. Gallina Toschi (in cc) as coordinator; the project actually includes gendered case studies where gender and sex variables are considered. You can find further information about our expertises, publications and the PLOTINA project at the following webpages: https://www.unibo.it/sitoweb/maria.rodriguez/, https://www.unibo.it/sitoweb/tullia.gallinatoschi/, http://www.unibo.it/en/research/projects-and-initiatives/research-projects-horizon-2020-1/plotina - Maria Teresa Rodriguez Estrada (2016).
  10. As you know, our current/developing interests are with "evolution" (species comparisons) and "environment" (hypoxia and cold exposure), but have also experience and interest in assessing effects of "lifestyle" (diet, exercise) and "aging" as well (in rodents). - Adam J Chicco (2016).
  11. Congratulations on the considerable progress with MITOEAGLE! I am keen to participate in this exciting project and I’m sure I can make a valuable contribution to it's success. I have extensive experience performing respirometry assays in human muscle biopsies, myoblasts/tubes and mouse tissues in the contexts of aging, obesity, exercise and inactivity. I feel that I could contribute to any of the five working groups, but particularly to WG2, WG4, and WG5. I am also happy and willing to serve on the Management Committee if needed. Again, congratulations on organizing such an exciting project. - Paul M Coen (2016).
  12. I would be interested to be involved with WG1 and WG2. In particular, I am interested in contributing to developing protocols for multicenter quality control among labs, and for permeabilized muscle bundle respirometry. - Harry B Rossiter (2016).
  13. I think that we could contribute via participation in the evaluation of the protocols, and helping to develop them further. In addition, we have been working on the establishing a database that would allow us to keep protocol details, cell isolation and several other aspects. There is nothing very advanced, but we could contribute to establishing such data collection practice. In addition, we, as opposed to many others, happen to use Strathkelvin respirometer. This alternative hardware would allow us to verify whether the protocols work on them as well. - Marko Vendelin (2016).


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