Difference between revisions of "Coupling-control ratio"

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Coupling-control ratio

Description

Coupling-control ratios, CCR, are flux control ratios, FCR, at a constant mitochondrial pathway-control state. In mitochondrial preparations, there are three well-defined coupling states of respiration: LEAK, OXPHOS, and Electron transfer pathway state (ET state). In these states, the corresponding respirtory rates are symbolized as L, P, and E. In living cells, the OXPHOS state cannot be induced, but in the ROUTINE state the respiration rate is R. A reference rate J_ref is defined by taking J_ref as the maximum flux, i.e. flux in the ET state, E, such that the lower and upper limits of the CCR are defined as 0.0 and 1.0. Then there are two mitochondrial CCR, L/E and P/E, and two CCR for living cells, L/E and R/E.

Abbreviation: CCR

Reference: Flux control ratio

The Blue Book 2020: Fig. 2.4.

Coupling-control ratios related to free capacities

L/P coupling control ratio, L/P

Free OXPHOS capacity, P-L

OXPHOS-coupling efficiency, 1-L/P

L/R coupling control ratio, L/R

Free ROUTINE activity, R-L

ROUTINE coupling efficiency, 1-L/R

LEAK-control ratio, L/E

Free ET capacity, E-L

ET-coupling efficiency, 1-L/E

netOXPHOS-control ratio, (P-L)/E

Free OXPHOS capacity, P-L

NetROUTINE control ratio, (R-L)/E

Free ROUTINE activity, R-L

More details » ET-coupling efficiency

Coupling-control ratios related to excess capacities

OXPHOS-control ratio, P/E

Excess E-P capacity, E-P

Excess E-P capacity factor, 1-P/E

ROUTINE-control ratio, R/E

Excess E-R capacity, E-R

Excess E-R capacity factor, 1-R/E

Compare

» Coupling control factor, CCF = 1-CCR

» Respiratory acceptor control ratio, RCR

References

Bioblast link	Reference	Year
Gnaiger 2020 BEC MitoPathways	Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5^th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002	2020
BEC 2020.1 doi10.26124bec2020-0001.v1	Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1	2020

Bioblast links: Normalization - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Rate

» Normalization of rate

» Flow

» Oxygen flow

» Flux

» Oxygen flux

» Flux control ratio

» Coupling-control ratio

» Pathway control ratio

» Flux control efficiency

Quantities for normalization

» Count in contrast to Number

» Mitochondrial marker

» O2k-Protocols: mitochondrial and marker-enzymes

» Citrate synthase activity

General

» Extensive quantity

» Specific quantity

» Advancement

» Motive unit

» Iconic symbols

Related keyword lists

» Keywords: Concentration and pressure

MitoPedia concepts: Respiratory control ratio

MitoPedia methods: Respirometry

@@ Line 1: / Line 1: @@
 {{MitoPedia
 |abbr=''CCR''
-|description='''Coupling-control ratios''', ''CCR'', are [[flux control ratio]]s, ''FCR'', at a constant mitochondrial [[pathway-control state]]. In mitochondrial preparations, there are three well-defined coupling states of respiration, ''L'', ''P'', ''E'' ([[LEAK]], [[OXPHOS]], [[Electron transfer pathway]]). In living cells, the OXPHOS state cannot be induced, but a [[ROUTINE]] state of respiration, ''R'', can be measured. The reference state, ''J''<sub>ref</sub>, is defined by taking ''J''<sub>ref</sub> as the maximum flux, i.e. flux in the ET state, ''E'', such that the lower and upper limits of the ''CCR'' are defined as 0.0 and 1.0. Then there are two mitochondrial ''CCR'', [[L/E|''L/E'']] and [[P/E|''P/E'']], and two ''CCR'' for living cells, [[L/E|''L/E'']] and [[ROUTINE-control ratio|''R/E'']].
+|description='''Coupling-control ratios''', ''CCR'', are [[flux control ratio]]s, ''FCR'', at a constant mitochondrial [[pathway-control state]]. In mitochondrial preparations, there are three well-defined coupling states of respiration: [[LEAK]], [[OXPHOS]], and [[Electron transfer pathway |Electron transfer pathway state]] (ET state). In these states, the corresponding respirtory rates are symbolized as ''L'', ''P'', and ''E''. In living cells, the OXPHOS state cannot be induced, but in the [[ROUTINE]] state the respiration rate is ''R''. A reference rate ''J''<sub>ref</sub> is defined by taking ''J''<sub>ref</sub> as the maximum flux, i.e. flux in the ET state, ''E'', such that the lower and upper limits of the ''CCR'' are defined as 0.0 and 1.0. Then there are two mitochondrial ''CCR'', [[L/E |''L/E'']] and [[P/E |''P/E'']], and two ''CCR'' for living cells, [[L/E |''L/E'']] and [[ROUTINE-control ratio |''R/E'']].
 |info=[[Flux control ratio]]
 }}
@@ Line 7: / Line 7: @@
 == Coupling-control ratios related to free capacities ==
 :::* [[L/P coupling control ratio |''L/P'' coupling control ratio]], ''L/P''
-:::: [[Free OXPHOS capacity]], ''≈P = P-L''
+:::: [[Free OXPHOS capacity]], ''P-L''
-:::: [[OXPHOS-coupling efficiency]], ''j<sub>≈P</sub>'' = ''≈P''/''P'' = 1-''L/P''
+:::: [[OXPHOS-coupling efficiency]], 1-''L/P''
 :::* [[L/R coupling control ratio |''L/R'' coupling control ratio]], ''L/R''
-:::: [[Free ROUTINE activity]], ''≈R = R-L''
+:::: [[Free ROUTINE activity]], ''R-L''
-:::: [[ROUTINE coupling efficiency]], ''j<sub>≈R</sub>'' = ''≈R''/''R'' = 1-''L/R''
+:::: [[ROUTINE coupling efficiency]], 1-''L/R''
 :::* [[LEAK-control ratio]], ''L/E''
-:::: [[Free ET capacity]], ''≈E = E-L''
+:::: [[Free ET capacity]], ''E-L''
-:::: [[ET-coupling efficiency]], ''j<sub>≈E</sub>'' = ''≈E''/''E'' = 1-''L/E''
+:::: [[ET-coupling efficiency]], 1-''L/E''
-:::* [[netOXPHOS-control ratio]], ''≈P/E''=(''P-L'')/''E''
+:::* [[netOXPHOS-control ratio]], (''P-L'')/''E''
-:::: [[Free OXPHOS capacity]], ''≈P = P-L''
+:::: [[Free OXPHOS capacity]], ''P-L''
-:::* [[NetROUTINE control ratio]], ''≈R/E''=(''R-L'')/''E''
+:::* [[NetROUTINE control ratio]], (''R-L'')/''E''
-:::: [[Free ROUTINE activity]], ''≈R = R-L''
+:::: [[Free ROUTINE activity]], ''R-L''
 :::* ''More details'' » [[ET-coupling efficiency]]
@@ Line 24: / Line 24: @@
 == Coupling-control ratios related to excess capacities ==
 :::* [[OXPHOS-control ratio]], ''P/E''
-:::: [[Excess E-P capacity |Excess ''E-P'' capacity]], ''ExP = E-P''
+:::: [[Excess E-P capacity |Excess ''E-P'' capacity]], ''E-P''
-:::: [[Excess E-P capacity factor |Excess ''E-P'' capacity factor]], ''j<sub>ExP</sub>'' = ''ExP''/''E'' = 1-''P/E''
+:::: [[Excess E-P capacity factor |Excess ''E-P'' capacity factor]], ''1-''P/E''
 :::* [[ROUTINE-control ratio]], ''R/E''
-:::: [[Excess E-R capacity |Excess ''E-R'' capacity]], ''ExR = E-R''
+:::: [[Excess E-R capacity |Excess ''E-R'' capacity]], ''E-R''
-:::: [[Excess E-R capacity factor |Excess ''E-R'' capacity factor]], ''j<sub>ExR</sub>'' = ''ExR''/''E'' = 1-''R/E''
+:::: [[Excess E-R capacity factor |Excess ''E-R'' capacity factor]], 1-''R/E''