MiPNet19.18A O2k-Series G: Start

MiPNet19.18A O2k-Series G: Start

Oxygraph-2k: start high-resolution respirometry.

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OROBOROS (2015-11-10) Mitochondr Physiol Network

Abstract: Gnaiger E, Fasching M, Gradl L, Gradl P (2015) Oxygraph-2k: start high-resolution respirometry. Mitochondr Physiol Network 19.18(A03):3-16. » Versions

» O2k-Videosupport

» Product: O2k, DatLab 6, O2k-Catalogue

• Keywords: HRR

• O2k-Network Lab: AT_Innsbruck_OROBOROS

Labels: MiParea: Instruments;methods

HRR: Oxygraph-2k, O2k-Manual

O2k-Core, O2k-SOP, DatLab

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

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Chamber assembly - possible problems

After assembly, medium is leaking out of the chamber

Siphon off medium and disassemble the chamber.
Check for apparent damages of the glass chamber, the POS seal tip and O-rings on chamber holders and stoppers. If any damage is visible, replace the part.
Reassemble the chamber and proceed with an O2 sensor test.

Oxygen leak of the chamber (strongly negative intercept in the instrumental background test
Unstable O2 signal
Unexpected high or low O2 signal

The possible contribution of chamber assembly is checked by removing the OroboPOS from the chamber and observing, if the signal changes to the expected values. - » Futher details.
If the O2k signal responds as expected, reassemble the O2k-chamber and proceed with an O2 sensor test.

Chamber disassembly

After siphoning off any medium from the chamber, remove the OroboPOS-Connector and O2k-Chamber Holder from the O2k.

Problem: Glass chamber stuck in the O2k, and the O2k-Chamber cannot be removed. It remains stuck even after washing with water.

This may be caused by spilled medium acting as an adhesive between glass chamber and copper block. Wash with plenty of warm water and try to remove the chamber while the O2k is switched on and heated up to 37 °C. Then GENTLY screw in the sensor holder (not the sensor connector) to push the glass chamber upwards (as you do during chamber_assembly.
If this does not work, increase the temperature to 40 °C (or slightly higher) for washing and continue with removing attempts.