MiPNet21.16 DatLab 7
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OROBOROS (2016-08-15) Mitochondr Physiol Network
Abstract: Gradl L, Gnaiger E, Capek O, Plattner C (2016) DatLab 7: innovations. Mitochondr Physiol Network 21.16
- » Product: DatLab, O2k, O2k-Catalogue
• O2k-Network Lab: AT_Innsbruck_OROBOROS
DatLab 7, our newly developed software, is a next step in high-resolution respirometry (HRR) incorporating legacy of previous DatLab versions and new, user-friendly features on quality control, documentation, and traceability of measurements with the Oroboros O2k.
MultiSensor data aquisition:
DatLab 7 is optimized for O2k-MultiSensor signal acquisition: oxygen, fluorescence (H2O2, mitochondrial membrane potential, calcium, ATP), ion selective electrodes, pX and nitric oxide sensors. At the heart of DatLab 7, a conceptual unification is obtained in calibrations and flux analysis of O2k-MultiSensor channels. As a result, multipoint calibration is now available for pX and for the amperometric channels.
Display and graphs:
Graphs are further improved for real-time display and publication. Versatile options allow an optimal set-up of the graphical display of data. Standard, Lab and User-defined display layouts can be stored and organized to better handle various projects, sample types and protocols.
Management of user accounts and experiments:
As a new feature, user accounts are assigned to each measurement for better traceability and customization. This facilitates the organization of experimental data and allows user-specific configurations in DatLab 7. A detailed description section, cohort, sample and subsample data in the “Experimental setup” improves organization and analysis of results.
DatLab- (DL-) Protocols:
Besides keeping the flexibility to run user-defined experiments, DatLab 7 is now equipped with the DL-Protocol function. A collection of evaluated and tested DL-Protocols are available (in .DLP format) providing all necessary information (events, marks, titration volume and concentration). The user is guided stepwise through the protocol (displayed in the DL-Protocol window), facilitating calibration steps, minimizing handling errors and greatly improving performance during O2k measurements. Moreover, an automatic warning at low oxygen levels can be set.
Events and marks:
Concentration and volume of the chemicals added during Substrate-uncoupler-inhibition-titration (SUIT) protocols can be stored together with event names and comments. Automatic outlier detection helps to avoid unwanted data points when setting marks during respiratory steady-states. On top the user has the option to delete, interpolate or restore data points of marks.
Mark statistics:
Numerous statistical functions are available for data analysis. “Marks statistics” organizes the marks and provides flexibility in displaying DL-Protocol marks, user-defined marks or both. The copy-to-clipboard function, with extended settings for backward compatibility, facilitates export to any third-party analysis software.
Experimental log:
An experimental log enables to view and generate detailed report of experimental-, software- and hardware settings for optimal tracking of each experiment.
Contextual help:
A hyperlink-based contextual help system provides optimal support throughout settings and experimental steps in DatLab 7. MitoPedia provides the background for this function, which warranties up-to-date and verified information anytime.
TIP2k control:
The software-driven, feedback-controlled Titration-Injection microPump (TIP2k) is an integral part of the O2k. In DatLab 7 TIP2k control has improved and facilitates user friendly and more flexible programming.
Labels: MiParea: Instruments;methods
HRR: O2k-Manual
O2k-Manual, O2k-Core, DatLab
DatLab O2k-Manuals
| Chapter | Section | Last update |
|---|---|---|
| MiPNet06.03 POS-calibration-SOP |  | 2023-02-06 |
| MiPNet12.10 TIP2k-manual | | 2021-07-08 |
| MiPNet14.06 Instrumental O2 background | | 2023-10-19 |
| MiPNet15.03 O2k-MultiSensor-ISE | | 2021-08-02 |
| MiPNet17.05 O2k-Fluo LED2-Module | | 2021-06-22 |
| MiPNet19.01 O2k-Core Manual Series F DatLab 5 | | 2014-03 |
| MiPNet19.18 O2k-Series G Core manual | | 2016-08-08 |
| MiPNet19.18A O2k-Series G: Start | | 2016-08-08 |
| MiPNet19.18C DatLab 6: Guide | | 2016-08-24 |
| MiPNet19.18E O2 flux analysis - DatLab 6 | | 2016-08-08 |
| MiPNet22.11 O2k-FluoRespirometer manual | | 2023-03-14 |
| MiPNet23.15 O2k-pH ISE-Module | | 2018-09-08 |
| MiPNet24.14 O2k-sV-Module manual | | 2020-03-17 |
| MiPNet26.06 DatLab 7: Guide | | 2021-06-02 |
