O2k-Demo experiments: Difference between revisions
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: ยป [[O2k-Catalogue: O2k-MultiSensor]] | : ยป [[O2k-Catalogue: O2k-MultiSensor]] | ||
: ยป [[O2k-Publications: O2k-Fluorometry]] | : ยป [[O2k-Publications: O2k-Fluorometry]] | ||
{{#ask: | {{#ask:[[Instrument and method::O2k-Protocol]] [[Additional label::O2k-Demo]] [[Additional label::O2k-MultiSensor]] | ||
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=== O2k-MultiSensor Manual === | === O2k-MultiSensor Manual === | ||
{{#ask: | {{#ask:[[Instrument and method::O2k-Manual]] [[Additional label::O2k-MultiSensor]] | ||
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Revision as of 11:00, 12 September 2018
O2k-Demo experiments |
Oroboros O2k demo
Protocol | View | Topic | Preparation | Tissue and cell | Mammal and model | Last update |
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Gnaiger 2020 BEC MitoPathways | published online 2020-12-30
| Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002 | Permeabilized cells Permeabilized tissue Homogenate Isolated mitochondria Intact cells | Heart Skeletal muscle Fibroblast | Human Mouse | 2020 |
MiPNet12.01 Suppl T-issue | ยปVersions | MitoPathways at the Q-junction: mouse skeletal muscle fibers. | Permeabilized tissue | Skeletal muscle | Mouse | 2018-11-18 |
MiPNet21.14 Reference sample HRR | ยปVersions | Development of a reference sample for HRR. | Intact cells Permeabilized cells | HEK | Human | 2016-10-19 |
MiPNet08.09 CellRespiration | ยปVersions | High-resolution respirometry and coupling-control protocol with living cells: ROUTINE, LEAK, ET-pathway, ROX. | Intact cells | Blood cells Lymphocyte | Human | 2016-08-11 |
MiPNet17.03 Shredder vs Fibers | ยปVersions | Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder. | Homogenate | Heart Liver | Fishes | 2015-06-18 |
MiPNet12.11 MitoRespiration | ยปVersions | Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control. | Isolated mitochondria | Artemia Crustaceans | 2014-02-20 | |
Pesta 2012 Methods Mol Biol | PMID: 22057559 | Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58. https://doi.org/10.1007/978-1-61779-382-0_3 | Permeabilized cells Permeabilized tissue Intact cells | Skeletal muscle Other cell lines HEK Fibroblast HUVEC | Human | 2012 |
MiPNet08.12 IOC22 | NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report. | Isolated mitochondria | Liver | Rat | 2011-12-11 | |
Gnaiger 2008 POS | Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial dysfunction in drug-induced toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52. | Permeabilized cells Permeabilized tissue Intact cells | Blood cells | Mouse | 2008 | |
MiPNet04.05 Titration-Injection | From step titration to ramp injection: Uncoupling by FCCP with TIP. | Intact cells | Endothelial;epithelial;mesothelial cell HUVEC | Human | 1999-01 |
O2k-Demo TIP2k
Protocol | View | Topic | Preparation | Tissue and cell | Mammal and model | Last update |
---|---|---|---|---|---|---|
Pesta 2012 Methods Mol Biol | PMID: 22057559 | Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58. https://doi.org/10.1007/978-1-61779-382-0_3 | Permeabilized cells Permeabilized tissue Intact cells | Skeletal muscle Other cell lines HEK Fibroblast HUVEC | Human | 2012 |
Gnaiger 2008 POS | Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial dysfunction in drug-induced toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52. | Permeabilized cells Permeabilized tissue Intact cells | Blood cells | Mouse | 2008 | |
MiPNet04.05 Titration-Injection | From step titration to ramp injection: Uncoupling by FCCP with TIP. | Intact cells | Endothelial;epithelial;mesothelial cell HUVEC | Human | 1999-01 |
TIP2k Manual
Chapter | View | Section | Last update |
---|---|---|---|
MiPNet12.10 TIP2k-manual | ยปVersions | Titration-Injection microPump TIP2k manual. | 2021-07-08 |
O2k-Demo O2k-MultiSensor
Protocol | View | Topic | Preparation | Tissue and cell | Mammal and model | Last update |
---|---|---|---|---|---|---|
Komlodi 2021 BEC AmR-O2 | Bioenerg Commun 2021.4. published online 2021-12-21
| Komlรณdi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. Bioenerg Commun 2021.4. https://doi.org/10.26124/bec:2021-0004 | Intact cells | Other cell lines | Saccharomyces cerevisiae | 2021 |
MiPNet24.11 mtMP calculation | ยป Versions | Mitochondrial membrane potential calculation | 2019-08-05 | |||
MiPNet20.14 AmplexRed H2O2-production | ยปVersions | O2k-FluoRespirometry: HRR and simultaneous determination of H2O2 production with Amplex UltraRed. | Isolated mitochondria | Heart | Mouse | 2019-06-24 |
MiPNet20.13 Safranin mt-membranepotential | ยปVersions | O2k-FluoRespirometry: HRR and simultaneous determination of mt-membrane potential with safranin or TMRM. | Homogenate | Nervous system | Mouse | 2019-06-24 |
MiPNet18.05 Amplex-Mouse-heart | ยปVersions | O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate. | Homogenate | Heart | Mouse | 2019-06-24 |
Krumschnabel 2015 Methods Mol Biol | PMID: 25631019 | Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. | Isolated mitochondria | Nervous system | Mouse | 2015 |
Makrecka-Kuka 2015 Biomolecules | Biomolecules 5:1319-38. PMID: 26131977 Open Access | Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. https://doi.org/10.3390/biom5031319 | Permeabilized cells Homogenate Isolated mitochondria Intact cells | Heart Nervous system HEK | Human Mouse | 2015 |
Krumschnabel 2014 Methods Enzymol | PMID: 24862266 ยป | Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. https://doi.org/10.1016/B978-0-12-416618-9.00009-1 | Homogenate | Nervous system | Mouse | 2014 |
MiPNet08.12 IOC22 | NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report. | Isolated mitochondria | Liver | Rat | 2011-12-11 |
O2k-MultiSensor Manual
Chapter | View | Section | Last update |
---|---|---|---|
MiPNet15.03 O2k-MultiSensor-ISE | ยปVersions | O2k-MultiSensor system with ion selective electrodes (ISE). | 2021-08-02 |
MiPNet15.05 NO-manual | ยปVersions | O2k-MultiSensor system with amperometric sensors: NO, H2S, H2O2. | 2016-04-21 |
MiPNet17.05 O2k-Fluo LED2-Module | ยปVersions | O2k-Fluo LED2-Module. | 2021-06-22 |
MiPNet23.15 O2k-pH ISE-Module | Measurement of proton flux with the O2k-pH ISE-Module. | 2018-09-08 | |
MiPNet24.12 NextGen-O2k: Q-Module | ยป Versions | NextGen-O2k: Q-Module manual | 2021-10-29 |
MiPNet26.11 NextGen-O2k: PB-Module | ยป Versions | NextGen-O2k: PB-Module manual | 2021-10-29 |
MiPNet26.12 NextGen-O2k: NADH-Module | ยป Versions | NextGen-O2k: NADH-Module manual | 2022-05-17 |
MiPNet28.09 O2k-Fluo Smart-Module manual | ยป Versions | O2k-Fluo Smart-Module manual | 2023-09-25 |
O2k-Guides: ยปO2k-Catalogue :: ยปO2k-Manual :: ยปO2k-Publications
O2k-Protocols ยป Link | |
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ยป O2k-Protocols: MitoPathways | |
ยป O2k-Demo experiments | |
ยป O2k-chemicals and media | |
ยป O2k-mitochondrial preparations | |
ยป O2k-Protocols: mitochondrial and marker-enzymes |