Difference between revisions of "P-L net OXPHOS capacity"

Revision as of 01:27, 2 June 2020

high-resolution terminology - matching measurements at high-resolution

P-L net OXPHOS capacity

Description

The free OXPHOS capacity, ≈P, is the OXPHOS capacity corrected for LEAK respiration, ≈P = P-L. ≈P is the scope for ADP stimulation, the respiratory capacity potentially available for phosphorylation of ADP to ATP. Oxygen consumption in the OXPHOS state, therefore, is partitioned into the free OXPHOS capacity, ≈P, strictly coupled to phosphorylation, ~P, and nonphosphorylating LEAK respiration, L_P, compensating for proton leaks, slip and cation cycling: P = ≈P+L_P. It is frequently assumed that LEAK respiration, L, as measured in the LEAK state, overestimates the LEAK component of respiration, L_P, as measured in the OXPHOS state, particularly if the protonmotive force is not adjusted to equivalent levels in L and L_P. However, if the LEAK component increases with enzyme turnover during P, the low enzyme turnover during L may counteract the effect of the higher Δp_mt.

Abbreviation: ≈P

Reference: Gnaiger 2014 MitoPathways

MitoPedia concepts: Respiratory state, Recommended

MitoPedia topics: EAGLE

Communicated by Gnaiger E 2014-08-09, edited 2015-01-19.

The Blue Book 2014: Fig. 2.4.

Coupling control states for ≈P

» Respiratory state

Reference state, Z_X: OXPHOS capacity, P = P´-ROX
Background state, Y_X: LEAK-respiration, L = L´-ROX
Metabolic control variable, X=Z_X-Y_X: Scope of ADP stimulation, free OXPHOS capacity, ≈P = P-L

Flux control factor

» Flux control factor, FCF

Coupling control factor, 1-Y_X/Z_X: P-L coupling control factor, OXPHOS coupling efficiency: j_≈P = ≈P/P =(P-L)/P = 1-L/P

Compare

Free ET capacity, ≈E = E-L

netOXPHOS control ratio, ≈P/E control ratio: ≈P/E = (P-L)/E

ET-coupling efficiency, E-L control factor: j_≈E = ≈E/E = (E-L)/E = 1-L/E

Intact cells: Free ROUTINE activity, ≈R = R-L

@@ Line 1: / Line 1: @@
 {{MitoPedia
 |abbr=''≈P''
-|description=[[Image:P-L.jpg|50 px|Free OXPHOS-capacity]] The '''free OXPHOS-capacity''', ''≈P'', is the [[OXPHOS-capacity]] corrected for [[LEAK respiration]], ''≈P'' = ''P-L''. ''≈P'' is the scope for ADP stimulation, the respiratory capacity potentially available for phosphorylation of ADP to ATP. Oxygen consumption in the OXPHOS state, therefore, is partitioned into the free OXPHOS-capacity, ''≈P'', strictly coupled to phosphorylation, ''~P'', and nonphosphorylating LEAK respiration, ''L<sub>P</sub>'', compensating for proton leaks, slip and cation cycling: ''P'' = ''≈P''+''L<sub>P</sub>''. It is frequently assumed that [[LEAK respiration]], ''L'', as measured in the LEAK state, overestimates the LEAK component of respiration, ''L<sub>P</sub>'', as measured in the OXPHOS state, particularly if the protonmotive force is not adjusted to equivalent levels in ''L'' and ''L<sub>P</sub>''. However, if the LEAK component increases with enzyme turnover during ''P'', the low enzyme turnover during ''L'' may counteract the effect of the higher Δ''p''<sub>mt</sub>.
+|description=[[Image:P-L.jpg|50 px|Free OXPHOS capacity]] The '''free OXPHOS capacity''', ''≈P'', is the [[OXPHOS capacity]] corrected for [[LEAK respiration]], ''≈P'' = ''P-L''. ''≈P'' is the scope for ADP stimulation, the respiratory capacity potentially available for phosphorylation of ADP to ATP. Oxygen consumption in the OXPHOS state, therefore, is partitioned into the free OXPHOS capacity, ''≈P'', strictly coupled to phosphorylation, ''~P'', and nonphosphorylating LEAK respiration, ''L<sub>P</sub>'', compensating for proton leaks, slip and cation cycling: ''P'' = ''≈P''+''L<sub>P</sub>''. It is frequently assumed that [[LEAK respiration]], ''L'', as measured in the LEAK state, overestimates the LEAK component of respiration, ''L<sub>P</sub>'', as measured in the OXPHOS state, particularly if the protonmotive force is not adjusted to equivalent levels in ''L'' and ''L<sub>P</sub>''. However, if the LEAK component increases with enzyme turnover during ''P'', the low enzyme turnover during ''L'' may counteract the effect of the higher Δ''p''<sub>mt</sub>.
 |info=[[Gnaiger 2014 MitoPathways]]
 }}
@@ Line 17: / Line 17: @@
 == Coupling control states for ''≈P'' ==
 ::» [[Respiratory state]]
-* [[Reference state]], ''Z<sub>X</sub>'': [[Image:P.jpg|link=OXPHOS-capacity|OXPHOS]] [[OXPHOS-capacity]], ''P'' = ''P´''-ROX
+* [[Reference state]], ''Z<sub>X</sub>'': [[Image:P.jpg|link=OXPHOS capacity|OXPHOS capacity]] [[OXPHOS capacity]], ''P'' = ''P´''-ROX
 * [[Background state]], ''Y<sub>X</sub>'': [[Image:L.jpg|link=LEAK-respiration|LEAK]] [[LEAK-respiration]], ''L'' = ''L´''-ROX
-* [[Metabolic control variable]], ''X=Z<sub>X</sub>-Y<sub>X</sub>'': [[Image:P-L.jpg|50 px|link=Free OXPHOS-capacity |Free OXPHOS-capacity]] Scope of ADP stimulation, [[free OXPHOS-capacity]], ''≈P'' = ''P-L''
+* [[Metabolic control variable]], ''X=Z<sub>X</sub>-Y<sub>X</sub>'': [[Image:P-L.jpg|50 px|link=Free OXPHOS capacity |Free OXPHOS capacity]] Scope of ADP stimulation, [[free OXPHOS capacity]], ''≈P'' = ''P-L''
@@ Line 28: / Line 28: @@
 == Compare ==
-:: [[Image:E-L.jpg|50 px|link=Free ET-capacity |Free ET-capacity]] [[Free ET-capacity]], ''≈E'' = ''E-L''
+:: [[Image:E-L.jpg|50 px|link=Free ET capacity |Free ET capacity]] [[Free ET capacity]], ''≈E'' = ''E-L''
 :: [[Image:NetP over E.jpg|60 px|link=NetOXPHOS control ratio |netOXPHOS control ratio]] [[netOXPHOS control ratio]], ''≈P/E'' control ratio: ''≈P/E'' = (''P-L'')/''E''
 :: [[Image:j--E.jpg|50 px|link=ETS coupling efficiency |ET-coupling efficiency]] [[ET-coupling efficiency]], ''E-L'' control factor: ''j<sub>≈E</sub>'' = ''≈E/E'' = (''E-L'')/''E'' = 1-''L/E''
 * Intact cells: [[Image:R-L.jpg|50 px|link=Free ROUTINE activity |Free ROUTINE activity]] [[Free ROUTINE activity]], ''≈R'' = ''R-L''