Difference between revisions of "SUIT-010"
From Bioblast
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::: '''[[SUIT protocol pattern]]:''' ce1;ce2Rot;ce3S;ce4D;1Dig;1c | ::: '''[[SUIT protocol pattern]]:''' ce1;ce2Rot;ce3S;ce4D;1Dig;1c | ||
__TOC__ | __TOC__ | ||
Communicated by [[Cardoso LH]] [[Iglesias-Gonzalez J]], [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2019-01-29) | Communicated by [[Cardoso LH]], [[Iglesias-Gonzalez J]], [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2019-01-29) | ||
== Specific SUIT protocols == | == Specific SUIT protocols == | ||
[[File:ce1;ce2Rot;ce3S;ce4D;1Dig;1c.png|400px|Respirometric test of optimum digitonin concentration ]] [[File:SUIT-010.png|500px]] | [[File:ce1;ce2Rot;ce3S;ce4D;1Dig;1c.png|400px|Respirometric test of optimum digitonin concentration ]] [[File:SUIT-010.png|500px]] |
Revision as of 15:05, 4 February 2019
Description
Abbreviation: ceS,S
Reference: A Optimization of digitonin concentration for pce »Versions
- SUIT-category: ceS,S
- SUIT protocol pattern: ce1;ce2Rot;ce3S;ce4D;1Dig;1c
Communicated by Cardoso LH, Iglesias-Gonzalez J, Huete-Ortega M and Gnaiger E (last update 2019-01-29)
Specific SUIT protocols
References
Year | Reference | Organism | Tissue;cell | |
---|---|---|---|---|
Doerrier 2018 Methods Mol Biol | 2018 | Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. https://doi.org/10.1007/978-1-4939-7831-1_3 | Human Mouse Rat Saccharomyces cerevisiae | Heart Skeletal muscle Endothelial;epithelial;mesothelial cell Blood cells HEK Platelet |
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
| ||
ce2Rot | ROX | ce1;ce2Rot
| ||
ce3S | SL(n) | S | CII | ce1;ce2Rot;ce3S
|
ce4D | SP | S | CII | ce1;ce2Rot;ce3S;ce4D
|
1Dig | SP | S | CII | ce1;ce2Rot;ce3S;ce4D;1Dig
|
1c | SP | S | CII | ce1;ce2Rot;ce3S;ce4D;1Dig;1c
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- Digitonin is a natural product and thus the effective concentration has to be determined by titrations for every batch. The optimum effective digitonin concentrations for complete plasma membrane permeabilization can be determined with this protocol.
Compare SUIT protocols
MitoPedia concepts:
MiP concept,
SUIT protocol,
Recommended
MitoPedia methods:
Respirometry