Difference between revisions of "State 2"

Revision as of 08:15, 23 June 2014

high-resolution terminology - matching measurements at high-resolution

State 2

Description

Substrate limited state of residual oxygen consumption, after addition of ADP to isolated mitochondria suspended in mitochondrial respiration medium in the absence of reduced substrates (ROX_D). Residual endogenous substrates are oxidized during a transient stimulation of oxygen flux by ADP. The peak – supported by endogenous substrates – is, therefore, a pre-steady state phenomenon preceding State 2. Subsequently oxygen flux declines to a low level (or zero) at the steady State 2 (Chance and Williams 1955). ADP concentration (D) remains high during ROX_D.

Abbreviation: ROX_D

Reference: Chance 1955 JBC-III, Gnaiger 2012 MitoPathways

MitoPedia methods: Respirometry, Spectrophotometry

MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.

The LEAK state of respiration: towards a concept-linked terminology of respiratory states

More details: >> LEAK respiration

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 {{MitoPedia
 |abbr=ROX<sub>D</sub>
-|description=Substrate limited state of [[residual oxygen consumption]], after addition of [[ADP]] to isolated mitochondria suspended in mitochondrial respiration medium in the absence of reduced substrates (ROX<sub>D</sub>).  Residual endogenous substrates are oxidized during a transient stimulation of oxygen flux by ADP. The peak – supported by endogenous substrates – is, therefore, a pre-steady state phenomenon preceding State 2. Subsequently oxygen flux declines to a low level (or zero) at the steady '''State 2''' ([[Chance_1955_JBC-III|Chance and Williams 1955]]). ADP concentration (D) remains high during ROX<sub>D</sub>.
+|description=[[File:ROX.jpg |link=Residual oxygen consumption]] Substrate limited state of [[residual oxygen consumption]], after addition of [[ADP]] to isolated mitochondria suspended in mitochondrial respiration medium in the absence of reduced substrates (ROX<sub>D</sub>).  Residual endogenous substrates are oxidized during a transient stimulation of oxygen flux by ADP. The peak – supported by endogenous substrates – is, therefore, a pre-steady state phenomenon preceding State 2. Subsequently oxygen flux declines to a low level (or zero) at the steady '''State 2''' ([[Chance_1955_JBC-III|Chance and Williams 1955]]). ADP concentration (D) remains high during ROX<sub>D</sub>.
 |info=[[Chance 1955 JBC-III]], [http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways]
 |type=Respiration