Difference between revisions of "Template:SUIT-003 O2 ce D061"
Line 15: | Line 15: | ||
|- | |- | ||
| ce2Omy | | (ce2Omy) | ||
| ''[[L]]'' | | ''[[L]]'' | ||
| ย | | ย | ||
| ย | | ย | ||
| ce1;ce2Omy | | ce1;(ce2Omy) | ||
{{Template:SUIT L(n)}} ย | {{Template:SUIT L(n)}} ย | ||
Line 28: | Line 28: | ||
| ย | | ย | ||
| | | | ||
| | | cce1;(ce2Omy);ce3U | ||
{{Template:SUIT U*}} {{Template:SUIT ET}} | {{Template:SUIT U*}} {{Template:SUIT ET}} | ||
Line 37: | Line 37: | ||
| ย | | ย | ||
| | | | ||
| ce1;ce2Omy;ce3U;ce4Rot | | ce1;(ce2Omy);ce3U;ce4Rot | ||
ย | |||
{{Template:SUIT ROX}} | {{Template:SUIT ROX}} | ||
|- | |- | ||
| | | ce5DMSO | ||
| | | ''[[ROX]]'' | ||
| ย | | ย | ||
| | | | ||
| ce1;ce2Omy;ce3U;ce4Rot; | | ce1;(ce2Omy);ce3U;ce4Rot;ce5DMSO | ||
{{Template:SUIT | {{Template:SUIT ROX}} | ||
|- | |- | ||
| | | ce6DMSO | ||
| ''[[ROX]]'' | | ''[[ROX]]'' | ||
| ย | | ย | ||
| | | | ||
| ce1;ce2Omy;ce3U;ce4Rot; | | ce1;(ce2Omy);ce3U;ce4Rot;ce5DMSO;ce6DMSO | ||
{{Template:SUIT ROX}} | {{Template:SUIT ROX}} | ||
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| ย | | ย | ||
| | | | ||
| ce1;ce2Omy;ce3U;ce4Rot; | | ce1;(ce2Omy);ce3U;ce4Rot;ce5DMSO;ce6DMSO;ce7Ama | ||
{{Template:SUIT ROX}} | {{Template:SUIT ROX}} | ||
|} | |} |
Revision as of 12:09, 26 June 2019
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state.
| ||
(ce2Omy) | L | ce1;(ce2Omy)
Non-phosphorylating resting state (LEAK state); LEAK respiration L(n) in the absence of ADP, ATP, AMP (no adenylates). | ||
ce3U | E | cce1;(ce2Omy);ce3U
Uncoupler titration (avoiding inhibition by high uncoupler concentrations) to obtain electron transfer (ET) capacity E (noncoupled ET-state). Test for limitation of OXPHOS capacity P by the phosphorylation system (ANT, ATP synthase, phosphate transporter) relative to ET capacity E in mt-preparations: E-P control efficiency and E-L coupling efficiency. In living cells: E-R control efficiency and E-L coupling efficiency. Noncoupled electron transfer state, ET state, with ET capacity E. | ||
ce4Rot | ROX | ce1;(ce2Omy);ce3U;ce4Rot
Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated either after inhibition of CIII (e.g. antimycin A, myxothiazol), CIV (e.g. Cyanide) or in the absence of endogenous fuel-substrates. Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration. | ||
ce5DMSO | ROX | ce1;(ce2Omy);ce3U;ce4Rot;ce5DMSO
Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated either after inhibition of CIII (e.g. antimycin A, myxothiazol), CIV (e.g. Cyanide) or in the absence of endogenous fuel-substrates. Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration. | ||
ce6DMSO | ROX | ce1;(ce2Omy);ce3U;ce4Rot;ce5DMSO;ce6DMSO
Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated either after inhibition of CIII (e.g. antimycin A, myxothiazol), CIV (e.g. Cyanide) or in the absence of endogenous fuel-substrates. Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration. | ||
ce7Ama | ROX | ce1;(ce2Omy);ce3U;ce4Rot;ce5DMSO;ce6DMSO;ce7Ama
Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated either after inhibition of CIII (e.g. antimycin A, myxothiazol), CIV (e.g. Cyanide) or in the absence of endogenous fuel-substrates. Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration. |