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Cardoso 2021 MitoFit MgG
| Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. MitoFit Preprints 2021.1. doi:10.26124/mitofit:2021-0001. - 2021-06-30 Published in »Bioenergetics Communications 2021.1« |
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Cardoso Luiza HD, Doerrier Carolina, Gnaiger Erich (2021-01-29) MitoFit Prep
Abstract: Journal publication 2021-06-30 in »Bioenergetics Communications 2021.1«
For the advanced study of mitochondrial function, high-resolution respirometry is extended by fluorometric measurement of ATP production using the fluorophore Magnesium Green™ (MgG). A common problem with several fluorescent dyes is the inhibition of mitochondrial respiration. In the present study, a coupling control protocol was applied in combination with MgG to measure ATP production simultaneously with respiration for calculation of P»/O2 ratios. MgG at 1.1 µM did not affect respiration through the NADH-linked and succinate-linked pathways. Respiration was not inhibited in any of the coupling control states, hence coupling control efficiencies were not affected by MgG.
• Keywords: ATP, ATP production, high-resolution respirometry, Magnesium Green, mitochondria, oxidative phosphorylation, fluorometry, FluoRespirometry • Bioblast editor: Gnaiger E • O2k-Network Lab: AT Innsbruck Oroboros
ORCID: 
Cardoso Luiza HD, Doerrier Carolina, Gnaiger Erich
Data availability
- Original files are available Open Access at Zenodo repository: 10.5281/zenodo.4032674
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Keywords
- Bioblast links: ATP production - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
Phosphorylation pathway
Phosphorylation pathway substrates
- » ADP
- » ATP
- » Inorganic phosphate
Phosphorylation pathway inhibitors
Coupling control
Respiratory complexes and coupling
ATP production measurement
Support
- Supported by project NextGen-O2k which has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 859770.
Labels: MiParea: Respiration, Instruments;methods
Organism: Mouse
Tissue;cell: Heart
Preparation: Isolated mitochondria
Regulation: ATP production, Coupling efficiency;uncoupling Coupling state: LEAK, OXPHOS, ET Pathway: N, S, ROX HRR: Oxygraph-2k, O2k-Fluorometer, NextGen-O2k
SUIT-006, MgG, MitoEAGLEPublication