Cardoso 2021 MitoFit MgG

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Cardoso 2021 MitoFit MgG

Publications in the MiPMap
Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. MitoFit Preprints 2021.1. doi:10.26124/mitofit:2021-0001

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Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration

Cardoso Luiza HD, Doerrier Carolina, Gnaiger Erich (2021-01-29) MitoFit Prep

Abstract: For the advanced study of mitochondrial function, high-resolution respirometry is extended by fluorometric measurement of ATP production using the fluorophore Magnesium Green™ (MgG). A common problem with several fluorescent dyes is the inhibition of mitochondrial respiration. In the present study, a coupling control protocol was applied in combination with MgG to measure ATP production simultaneously with respiration for calculation of P»/O2 ratios. MgG at 1.1 µM did not affect respiration through the NADH-linked and succinate-linked pathways. Respiration was not inhibited in any of the coupling control states, hence coupling control efficiencies were not affected by MgG.

Keywords: ATP, ATP production, high-resolution respirometry, Magnesium Green, mitochondria, oxidative phosphorylation, fluorometry, FluoRespirometry Bioblast editor: Gnaiger E O2k-Network Lab: AT Innsbruck Oroboros

ORCID: ORCID.png Cardoso Luiza HD, ORCID.png Doerrier Carolina, ORCID.png Gnaiger Erich

References

LinkReferenceViewYear
Budinger GRS, Duranteau J, Chandel NS, Schumacker PT (1998) Hibernation during hypoxia in cardiomyocytes. Role of mitochondria as the O2 sensor. J Biol Chem 273(6):3320-6.PMID:94524491998
Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. I. Kinetics of oxygen utilization. J Biol Chem 217:383-93.PMID: 13271402 Open Access1955
Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48.PMID: 24862274»O2k-brief2014
Chinopoulos C, Vajda S, Csanady L, Mandi M, Mathe K, Adam-Vizi V (2009) A Novel Kinetic Assay of Mitochondrial ATP-ADP Exchange Rate Mediated by the ANT. Biophys J 96, 2490-504.PMID: 243911342009
Devaux JBL, Hedges CP, Birch N, Herbert N, Renshaw GMC, Hickey AJR (2019) Acidosis maintains the function of brain mitochondria in hypoxia-tolerant triplefin fish: a strategy to survive acute hypoxic exposure? Front Physiol 9:1941.PMID:307135042019
Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70.PMID: 29850993 »O2k-brief
O2k-Protocols
2018
Fink BD, Bai F, Yu L, Sivitz WI (2017) Regulation of ATP production: dependence on calcium concentration and respiratory state. Am J Physiol Cell Physiol 313:C146-53.PMID: 28515085»O2k-brief2017
Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97.PMID: 11718759
Bioblast pdf
2001
Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2: 112 pp. doi:10.26124/bec:2020-0002Bioblast pdfPublished online: 2020-Dec-30

2020
Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Heidelberg, Berlin, New York:431-42.Bioblast pdf - Springer link
O2k-Protocols contents
2000
Gnaiger E, Méndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97:11080-5.PMID: 11005877 Open Access2000
Gnaiger E, Wyss M (1994) Chemical forces in the cell: Calculation for the ATP system. In: What is Controlling Life? (Gnaiger E, Gellerich FN, Wyss M, eds) Modern Trends in BioThermoKinetics 3. Innsbruck Univ Press:207-12.
BTK1994
Bioblast pdf
1994
Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1:44 pp. doi:10.26124/bec:2020-0001.v1BEC 2020.1 pdfPublished online: 2020-May-20

2020
Goo S, Pham T, Han JC, Nielsen P, Taberner A, Hickey A, Loiselle D (2013) Multiscale measurement of cardiac energetics. Clin Exp Pharmacol Physiol 40:671-81.PMID: 237459442013
Horgan DJ (1978) A spectrophotometric assay of ATP synthesized by sarcoplasmic reticulum. Aust J Biol Sci 31(1):21-4.PMID:981391978
Iftikar FI, Hickey AJ (2013) Do mitochondria limit hot fish hearts? Understanding the role of mitochondrial function with heat stress in Notolabrus celidotus. PLoS One 8:e64120.PMID: 23724026 Open Access2013
Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81.PMID: 24862266 »O2k-brief
O2k-Protocols contents
2014
Laboratory protocol: isolation of mouse heart mitochondria.Bioblast pdf »Versions
O2k-Protocols
2015-06-22
Lark DS, Torres MJ, Lin CT, Ryan TE, Anderson EJ, Neufer PD (2016) Direct real-time quantification of mitochondrial oxidative phosphorylation efficiency in permeabilized skeletal muscle myofibers. Am J Physiol Cell Physiol 311:C239-45.PMID: 27335172 Open Access2016
Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840, DOI:10.1038/s41598-017-02789-8.PMID: 28588260 Sci Rep Open Access2017
Leyssens A, Nowicky AV, Patterson L, Crompton M, Duchen MR (1996) The relationship between mitochondrial state, ATP hydrolysis, [Mg2+]i and [Ca2+]i studied in isolated rat cardiomyocytes. J Physiol 496:111-28.1996
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265-75.PMID:149077131951
Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38.PMID: 26131977 Open Access
O2k-Protocols contents
2015
Manfredi G, Yang L, Gajewski CD, Mattiazzi M (2002) Measurements of ATP in mammalian cells. Methods 26(4):317-26.PMID: 120549222002
Masson SWC, Hedges CP, Devaux JBL, James CS, Hickey AJR (2017) Mitochondrial glycerol 3-phosphate facilitates bumblebee pre-flight thermogenesis. Sci Rep 7:13107.PMID: 29026172 Open Access2017
Menegollo M, Tessari I, Bubacco L, Szabadkai G (2019) Determination of ATP, ADP, and AMP Levels by Reversed-Phase High-Performance Liquid Chromatography in Cultured Cells. Methods Mol Biol 1925:223-32.PMID:306740302019
Morciano G, Sarti AC, Marchi S, Missiroli S, Falzoni S, Raffaghello L, Pistoia V, Giorgi C, Di Virgilio F, Pinton P (2017) Use of luciferase probes to measure ATP in living cells and animals. Nat Protoc 12(8):1542-62.PMID:286830622017
Napa K, Baeder AC, Witt JE, Rayburn ST, Miller MG, Dallon BW, Gibbs JL, Wilcox SH, Winden DR, Smith JH, Reynolds PR, Bikman BT (2017) LPS from P. gingivalis negatively alters gingival cell mitochondrial bioenergetics. Int J Dent 2017:2697210.Open Access2017
Pham T, Loiselle D, Power A, Hickey AJ (2014) Mitochondrial inefficiencies and anoxic ATP hydrolysis capacities in diabetic rat heart. Am J Physiol 307:C499–507.PMID: 24920675 Open Access2014
Power A, Pearson N, Pham T, Cheung C, Phillips A, Hickey A (2014) Uncoupling of oxidative phosphorylation and ATP synthase reversal within the hyperthermic heart. Physiol Rep pii:e12138.PMID: 252632022014
Salin K, Villasevil EM, Anderson GJ, Lamarre SG, Melanson CA, McCarthy I, Selman C, Metcalfe NB (2019) Differences in mitochondrial efficiency explain individual variation in growth performance. Proc Biol Sci 286:20191466.PMID: 31431161 Open Access2019
Salin K, Villasevil EM, Anderson GJ, Selman C, Chinopoulos C, Metcalfe NB (2018) The RCR and ATP/O indices can give contradictory messages about mitochondrial efficiency. Integr Comp Biol 58:486-94.PMID: 299826162018
Salin K, Villasevil EM, Auer SK, Anderson GJ, Selman C, Metcalfe NB, Chinopoulos C (2016) Simultaneous measurement of mitochondrial respiration and ATP production in tissue homogenates and calculation of effective P/O ratios. Physiol Rep 10.14814/phy2.13007.PMID: 27798358 Open access2016
Sausen CW, Rogers CM, Bochman ML (2019) Thin-Layer Chromatography and Real-Time Coupled Assays to Measure ATP Hydrolysis. Methods Mol Biol 1999:245-253.PMID:311275812019
Scaduto RC Jr, Grotyohann LW (1999) Measurement of mitochondrial membrane potential using fluorescent rhodamine derivatives. Biophys J 76:469-77.PMID:98761591999
Selected media and chemicals for respirometry with mitochondrial preparations.Bioblast pdf  »Versions
O2k-Protocols
2016-08-30

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Bioblast links: ATP production - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Phosphorylation pathway

» Phosphorylation pathway
» ATP synthase
» Adenine nucleotide translocase
» Phosphate carrier

Phosphorylation pathway substrates

» ADP
» ATP
» Inorganic phosphate

Phosphorylation pathway inhibitors

» Oligomycin
» Carboxyatractyloside
» Atractyloside
» Bongkrekik acid

Coupling control

» Oxidative phosphorylation
» OXPHOS capacity
» P-L control efficiency
» ROUTINE respiration
» R-L control efficiency

Respiratory complexes and coupling

» Complex I
» Complex III
» Complex IV
» Proton pump
» Electron-transfer-pathway state

ATP production measurement

» Magnesium Green



Labels: MiParea: Respiration, Instruments;methods 


Organism: Mouse  Tissue;cell: Heart  Preparation: Isolated mitochondria 

Regulation: ATP production, Coupling efficiency;uncoupling  Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, ROX  HRR: Oxygraph-2k, O2k-Fluorometer 

SUIT-006, MgG, MitoEAGLEPublication