O2k-Open Support

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O2k-Open Support

We provide service and support based on open innovation

O2k-Open Support: technical help

O2k-Open Support includes Open Access pages that help to optimally use the O2k, to apply instrumental O2k-Quality Control, and perform user-service when needed.

1. MitoPedia: O2k: find information quickly with MitoPedia, the Bioblast glossary with lists of key words and links to relevant pages.
   1. MitoPedia: O2k-Open Support
   2. MitoPedia: O2k hardware
   3. MitoPedia: DatLab
2. O2k-Procedures: explain various applications of the O2k with O2k-Demo experiments
3. O2k-Manual: topic specific manuals with step by step help
4. O2k-Videosupport
5. O2k-Products: is not merely a 'List of products', it presents a guided tour to the O2k and integrates the concept of Gentle Science and Open Science.

O2k-Quality Control

Many commonly raised issues of O2k-technical support are solved in the newest version of DatLab.

The O2 sensor and O2k chamber tests are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of solution protocols (preparation of chemicals), sample preparation protocols (such as mitochondrial preparations), and SUIT protocols.

1. Clean O2k-chambers and contamination-free incubation medium are required.
2. Analysis of DatLab files recorded in instrumental QC tests is essential.
3. Biological experiments are not suitable for trouble shooting.

The sequence of instrumental QC tests is strictly

1. O2 sensor test

The OroboPOS (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:

» O2 sensor test: QC1: Oxygen sensor test

2. O2k-chamber test

If all quality control criteria of the O2 sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail:

» O2k-chamber test: QC2: Instrumental O₂ background

O2k-Open Support agreement

The Oroboros experts will be happy to help in using our O2k-Open Support system.

» Search for defective O2k components

» In case you need further technical support and for your valuable feedback, please contact support@oroboros.at

» O2k-Open Support agreement

» O2k repair

The O2k-Network

The Oroboros O2k-Network serves to connect and support: Contact for advice on applications of the O2k and high-resolution respirometry - the global network in mitochondrial physiology.

» The O2k-Network Labs: » O2k-Network

» O2k-Network discussion forum

» Oroboros O2k-Laboratory - open for innovation and cooperation.

» Oroboros Science Scholarships - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry