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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
Mracek Tomas
MitoPedia topics: EAGLE
COST: Member
COST WG4: WG4
| Name | Mracek Tomas, RNDr.
Head of laboratory |
|---|---|
| Institution |  Institute of Physiology, Czech Academy of Sciences, CZ |
| Address | Videnska 1083, 142 20 |
| City | Prague |
| State/Province | |
| Country | Czech Republic |
| [email protected] | |
| Weblink | |
| O2k-Network Lab | CZ Prague Houstek J |
Labels:
Publications
| Published | Reference | |
|---|---|---|
| Markovic 2022 Biomedicines | 2022 | Marković A, Tauchmannová K, Šimáková M, Mlejnek P, Kaplanová V, Pecina P, Pecinová A, Papoušek F, Liška F, Šilhavý J, Mikešová J, Neckář J, Houštěk J, Pravenec M, Mráček T (2022) Genetic complementation of ATP synthase deficiency due to dysfunction of TMEM70 assembly factor in rat. https://doi.org/10.3390/biomedicines10020276 |
| Pajuelo 2020 Cells | 2020 | Pajuelo Reguera D, Čunátová K, Vrbacký M, Pecinová A, Houštěk J, Mráček T, Pecina P (2020) Cytochrome c oxidase subunit 4 isoform exchange results in modulation of oxygen affinity. Cells 9:443. |
| BEC 2020.1 doi10.26124bec2020-0001.v1 | 2020 | Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1 |
| Pajuelo-Reguera 2020 Cells | 2020 | Pajuelo Reguera David, Čunátová Kristýna, Vrbacký Marek, Pecinová Alena, Houštěk Josef, Mráček Tomáš, Pecina Petr (2020) Cytochrome c oxidase subunit 4 isoform exchange results in modulation of oxygen affinity. Cells 9:E443. |
| Pecinova 2017 Oxid Med Cell Longev | 2017 | Pecinova A, Drahota Z, Kovalcikova J, Kovarova N, Pecina P, Alan L, Zima M, Houstek J, Mracek T (2017) Pleiotropic effects of biguanides on mitochondrial reactive oxygen species production. Oxid Med Cell Longev 7038603. |
| Pravenec 2017 Physiol Res | 2017 | Pravenec M1, Zídek V, Landa V, Mlejnek P, Šilhavý J, Šimáková M, Trnovská J, Škop V, Marková I, Malínská H, Hüttl M, Kazdová L, Bardová K, Tauchmannová K, Vrbacký M, Nůsková H, Mráček T, Kopecký J, Houštěk J (2017) Mutant Wars2 gene in spontaneously hypertensive rats impairs brown adipose tissue function and predisposes to visceral obesity. Physiol Res 66:917-24. |
| Melenovsky 2016 Eur J Heart Fail | 2016 | Melenovsky V, Petrak J, Mracek T, Benes J, Borlaug BA, Nuskova H, Pluhacek T, Spatenka J, Kovalcikova J, Drahota Z, Kautzner J, Pirk J, Houstek J (2016) Myocardial iron content and mitochondrial function in human heart failure: a direct tissue analysis. Eur J Heart Fail 19:522-30. |
| Pravenec 2016 Physiol Genomics | 2016 | Pravenec M, Mlejnek P, Zídek V, Landa V, Šimáková M, Šilhavý J, Strnad H, Eigner S, Eigner Henke K, Škop V, Malínská H, Trnovská J, Kazdová L, Drahota Z, Mráček T, Houštěk J (2016) Autocrine effects of transgenic resistin reduce palmitate and glucose oxidation in brown adipose tissue. Physiol Genomics 48:420-7. |
| Hartmannova 2016 Hum Mol Genet | 2016 | Hartmannová H, Piherová L, Tauchmannová K, Kidd K, Acott PD, Crocker JF, Oussedik Y, Mallet M, Hodaňová K, Stránecký V, Přistoupilová A, Barešová V, Jedličková I, Živná M, Sovová J, Hůlková H, Robins V, Vrbacký M, Pecina P, Kaplanová V, Houštěk J, Mráček T, Thibeault Y, Bleyer AJ, Kmoch S (2016) Acadian variant of Fanconi syndrome is caused by mitochondrial respiratory chain complex I deficiency due to a non-coding mutation in complex I assembly factor NDUFAF6. Hum Mol Genet 25:4062-79. |
| Hejzlarova 2015 Biochem J | 2015 | Hejzlarová K, Kaplanová V, Nůsková H, Kovářová N, Ješina P, Drahota Z, Mráček T, Seneca S, Houštěk J (2015) Alteration of structure and function of ATP synthase and cytochrome c oxidase by lack of Fo-a and Cox3 subunits caused by mitochondrial DNA 9205delTA mutation. Biochem J 466:601-11. |
| Kluckova 2015 Cell Death Dis | 2015 | Kluckova K, Sticha M, Cerny J, Mracek T, Dong L, Drahota Z, Gottlieb E, Neuzil J, Rohlena J (2015) Ubiquinone-binding site mutagenesis reveals the role of mitochondrial complex II in cell death initiation. Cell Death Dis 6:e1749. |
| Pecina 2014 Biochim Biophys Acta Clinical | 2014 | Pecina P, Houstkova H, Mracek T, Pecinova A, Nuskova H.Tesarova M, Hansikova H, Janota J, Zemanc J, Houstek J (2014) Noninvasive diagnostics of mitochondrial disorders in isolated lymphocytes with high resolution respirometry. Biochim Biophys Acta Clinical 2:62–71. |
| Mracek 2013 Biochim Biophys Acta | 2012 | Mráček T, Drahota Z, Houstek J (2013) The function and the role of the mitochondrial glycerol-3-phosphate dehydrogenase in mammalian tissues. Biochim Biophys Acta 1827:401-10. https://doi.org/10.1016/j.bbabio.2012.11.014 |
| Rauchova 2011 Horm Metab Res | 2010 | Rauchová H, Mrácek T, Novák P, Vokurková M, Soukup T (2010) Glycerol-3-phosphate dehydrogenase expression and oxygen consumption in liver mitochondria of female and male rats with chronic alteration of thyroid status. Horm Metab Res 43:43-7. |
| Vrbacky 2007 Biochim Biophys Acta | 2007 | Vrbacky M, Drahota Z, Mracek T, Vojtískova A, Jesina P, Stopka P, Houstek J (2007) Respiratory chain components involved in the glycerophosphate dehydrogenase-dependent ROS production by brown adipose tissue mitochondria. Biochim Biophys Acta 1767:989-97. |
| Honzik 2006 Placenta | 2006 | Honzik T, Drahota Z, Bohm M, Jesina P, Mracek T, Paul J, Zeman J, Houstek J (2006) Specific properties of heavy fraction of mitochondria from human-term placenta - Glycerophosphate-dependent hydrogen peroxide production. Placenta 27:348-56. |
| Drahota 2002 J Bioenerg Biomembr | 2002 | Drahota Z, Chowdhury SK, Floryk D, Mrácek T, Wilhelm J, Rauchova H, Lenaz G, Houstek J (2002) Glycerophosphate-dependent hydrogen peroxide production by brown adipose tissue mitochondria and its activation by ferricyanide. J Bioenerg Biomembr 34:105-13. |
Abstracts
| Published | Reference | |
|---|---|---|
| Mracek 2017 MiP2017 | 2017 | |
| Pecinova 2017 MiP2017 | 2017 |  |
| Pecina 2017 MiP2017 | 2017 |  |
| Pajuelo-Reguera 2017 MiP2017 | 2017 |  |
| Kovalcikova 2017 MiP2017 | 2017 |  |
| Efimova 2017 MiP2017 | 2017 |  |
| Cunatova 2017 MiP2017 | 2017 |  |
| Brazdova 2017 MiP2017 | 2017 |  |
| Alan 2017 MiP2017 | 2017 |  |
| Pecinova 2015 Abstract MiP2015 | 2015 | Autocrine effects of transgenic resistin reduce palmitate and glucose oxidation in brown adipose tissue. |
| Karbanova 2015 Abstract MiP2015 | 2015 | CD36 does not directly participate in mitochondrial fatty acid transport and oxidation. |
| Kovarova 2015 Abstract MiP2015 | 2015 | Tissue- and species-specific differences in cytochrome c oxidase assembly induced by SURF1 defects. |
| Tauchmannova 2015 Abstract MiP2015 | 2015 | Assembly of subunit Fo-a into mammalian ATP synthase. |
| Pecina 2015 Abstract MiP2015 | 2015 | Pharmacological inhibition of fatty-acid oxidation synergistically enhances the effect of L-asparaginase in childhood ALL cells. |
| Kovalcikova 2015 Abstract MiP2015 | 2015 | Derangements of myocardial mitochondrial function in patients with end-stage heart failure is associated with reduced endonuclease G. |
| Mracek 2015 Abstract MiP2015 | 2015 | Myocardial iron and mitochondrial function in failing and non-failing human heart: direct tissue analysis. |
| Rohlena 2015 Abstract MiP2015 | 2015 | Ubiquinone-binding site mutagenesis reveals the role of mitochondrial complex II in cell death initiation. |
| Nuskova 2015 Abstract MiPschool London 2015 | 2015 | Insufficient energy provision or increased oxidative stress – what matters more in ATP synthase deficiencies? |
| Houstek 2014 Abstract MiP2014 | 2014 | Alteration of structure and function of ATP synthase and cytochrome c oxidase by lack of Fo-a and Cox3 subunits caused by mitochondrial DNA 9205delTA mutation. |
| Pecina 2014 Abstract MiP2014 | 2014 | Manifestation of mitochondrial disorders of nuclear origin in lymphocytes. |
| Mracek 2014 Abstract MiP2014 | 2014 | Function of mitochondrial energy provision apparatus is compromised in patients with chronic heart failure. Mitochondr Physiol Network 19.13. |
| Pecina 2013 Abstract MiP2013 | 2013 | Pecina P, Houšťková H, Mráček T, Pecinová A, Nůsková H, Tesařová M, Hansíková H, Janota J, Zeman J, Houštěk J (2013) The use of lymphocytes for diagnostics of mitochondrial oxidative phosphorylation disorders. Mitochondr Physiol Network 18.08. |
MitoEAGLE
- Benefits for COST and for the COST Action MitoEAGLE
- Thinking about our potential involvement, I would propose following:
- WG3 - we can easily squeeze here with comparison of Oroboros and Seahorse, which we try to do anyway in order to establish the value of Seahorse. Here, we can join N. Larssons group, if it joins mtEAGLE (did not find them here yet).
- WG3/WG1 – Measurement of lymphocytes and using lymphocytes for cross-laboratory validation –as we now have quite extensive experience with lymphoid cells, we can participate in protocol development and so on. We are also happy to host some visiting researchers and teach them our expertise.
- WG1 – we have just about started screening of mito phenotypes in rat RI strains (SHRxBN). In the end, data will be available in http://www.genenetwork.org/ database, but most likely as some ratios of various respiratory states to make them usable for QTL mapping. We can deposit original data into mitoEAGLE database as well. It will be more genotype x phenotype study than age, gender etc. x phenotype and it would include rats, which would require broadening the mitoEAGLE scope to laboratory rodents in general and not to keep it focussed on mice only.
- If there would be any chance to utilise “Short-Term Scientific Missions” to send young team members to other participating labs and learn some new techniques (just looking at the participant list, e.g. fluorescent measuring of ATP in the lab of C. Chinopoulos springs into mind), it would be great added value of this COST project for us. And of course, we are also happy to host others in our lab, as I proposed with lymphocyte programme (and it does not have to be limited to it).
