Difference between revisions of "MitoEAGLE data: blood and cultured cells"
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:::: Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine. | :::: Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine. | ||
:::: A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2). | :::: A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2). | ||
== WG4 Participants == | |||
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Revision as of 14:55, 27 April 2016
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News and Events |  |
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Working Groups | Short-Term Scientific Missions | Management Committee | Members |  |
COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
| Country | City | Contact |
|---|---|---|
| Armenia | Yerevan | Trchounian K |
| Austria | Innsbruck | Fischer Christine |
| Austria | Innsbruck | Hoppel Florian |
| Austria | Innsbruck | Meszaros Andras T |
| Austria | Innsbruck | Torres-Quesada Omar |
| Austria | Innsbruck | Gnaiger Erich |
| Austria | Innsbruck | Cardoso Luiza HD |
| Austria | Innsbruck | Garcia-Souza Luiz F |
| Austria | Innsbruck | Doerrier Carolina |
| Austria | Innsbruck | Karabatsiakis Alexander |
| Belgium | Namur | Detraux Damien |
| Belgium | Leuven | Bird Matthew |
| Belgium | Namur | Arnould Thierry |
| Belgium | Liege | Votion Dominique-Marie |
| Belgium | Namur | Renard P |
| Bosnia and Herzegovina | Tuzla | Jusic Amela |
| Bosnia and Herzegovina | Tuzla | Hajrulahovic Anesa |
| Brazil | Sao Paulo | Verdaguer Ignasi Bofill |
| Brazil | Sao Paulo | Dominguez-Amorocho OA |
| Brazil | Sao Paulo | Alencar Mayke Bezerra |
| Brazil | São Paulo | Crispim Marcell |
| Croatia | Zagreb | Rubelj Ivica |
| Czech Republic | Prague 4 | Bajzikova Martina |
| Czech Republic | Prague | Pecina Petr |
| Czech Republic | Prague | Mracek Tomas |
| Czech Republic | Prague | Hansikova Hana |
| Czech Republic | Vestec | Zobalova Renata |
| Czech Republic | Pilsen | Kripnerova M |
| Czech Republic | Pilsen | Markova Michaela |
| Czech Republic | Prague | Elkalaf Moustafa |
| Czech Republic | Hradec Kralove | Vernerova Andrea |
| Czech Republic | Prague | Fisar Zdenek |
| Czech Republic | Hradec Kralove | Maseko Tumisang Edward |
| Czech Republic | Pilsen | Kuncova J |
| Czech Republic and Australia | Prague and Southport | Neuzil Jiri |
| Czech rep | Hradec Kralove | Falaye T |
| Denmark | Copenhagen N | Mortensen OH |
| Denmark | Copenhagen | Rasmussen LJ |
| Denmark | Copenhagen | Desler C |
| Denmark | Hvidovre | Nehlin Jan O |
| Denmark | Aarhus | Rattan Suresh IS |
| Denmark | Malov | Hey-Mogensen M |
| Egypt | Cairo | Abdel-Rahman Engy Ali |
| Egypt | Cairo | Ali Sameh S |
| Estonia | Tallinn | Truu Laura |
| Estonia | Tallinn | Klepinina Lyudmila |
| Estonia | Tartu | Grellier T |
| Finland | Helsinki | Pirinen E |
| Finland | Jyvaeskylae | Lehti Maarit |
| Finland | Turku | Stier Antoine |
| France | Bordeaux cedex | Mourier A |
| France | Bron | Rizwan Alam M |
| France | Paris | Petit Patrice X |
| Germany | Neuherberg | Borchard Sabine |
| Germany | Ulm | Kolassa Iris |
| Germany | Freising | Klingenspor Martin |
| Germany | Gießen | Silaidos Carmina |
| Germany | Düsseldorf | Prigione Alessandro |
| Germany | Leipzig | Ost Mario |
| Germany | Duesseldorf | Piel Sarah |
| Germany | Regensburg | Renner-Sattler Kathrin |
| Germany | Freising | Fromme Tobias |
| Germany | Freising | Keppner Gloria |
| Germany | Mainz | Vujacic-Mirski Ksenija |
| Germany | Giessen | Eckert Gunter P |
| Germany | Munich | Jastroch M |
| Germany | Mainz | Kalinovic S |
| Germany | Magdeburg | Vieira Ligo Teixeira Camila |
| Germany | Biberach an der Riß | Nold V |
| Germany | Hamburg | Singer Dominique |
| Germany | Freising | Schoettl T |
| Germany | Duesseldorf | Eckers A |
| Germany | Ulm | Schaefer PM |
| Germany | Freising-Weihenstephan | Musiol Eva |
| Germany | Ulm | Calzia Enrico |
| Germany | Ulm | Hartmann C |
| Greece | Athens | Trougakos Ioannis P |
| Hungary | Debrecen | Verebne Tar K |
| Hungary | Budapest | Tretter Laszlo |
| Hungary | Budapest | Komlodi Timea |
| Ireland | Dublin | Porter Richard K |
| Ireland | Sligo | Murphy J |
| Ireland | Dublin | Karavyraki Marilena |
| Israel | Rishon Le Zion | Hachmo Yafit |
| Israel | Jerusalem | Saada Reisch Ann |
| Israel | Zrifin | Sova Marina |
| Italy | Milano | Bottoni P |
| Italy | Verona | Calabria Elisa |
| Italy | Bologna | Genova Maria Luisa |
| Italy | Bolzano | Pichler Irene |
| Italy | Bologna | Paterlini S |
| Italy | Bolzano/Bozen | Volani Chiara |
| Italy | Ancona | Battino Maurizio |
| Italy | Bologna | Porcelli AM |
| Italy | Rome | Scatena Roberto |
| Italy | Padova | Fernandez-Vizarra Erika |
| Italy | Bologna | Gasparre G |
| Italy | Bolzano | Castelo Rueda Maria Paulina |
| Japan | Sapporo | Shirakawa R |
| Latvia | Riga | Makarova Elina |
| Latvia | Riga | Kidere Dita |
| Malaysia | Kuala Lumpur | Abdul Karim Norwahidah |
| Malta | Msida | Vella Joanna |
| Netherlands | Nijmegen | Koopman Werner JH |
| Netherlands | Wageningen | Keijer Jaap |
| Netherlands | Groningen | Rots MG |
| Norway | Oslo | Eide L |
| Norway | Oslo | Lund Jenny |
| Norway | Oslo | Nikolic N |
| Norway | Oslo | Rustan AC |
| Norway | Bergen | Tronstad Karl Johan |
| Palestine | Ramallah | Stiban Johnny |
| Poland | Lodz | Labieniec-Watala Magdalena |
| Poland | Lodz | Siewiera Karolina |
| Poland | Poznań | Ludziejewska A |
| Poland | Warsaw | Wasilewski M |
| Poland | Krakow | Kaczara P |
| Poland | Poznan | Michalak Slawomir |
| Poland | Poznan | Swiniuch Daria |
| Poland | Warsaw | Dymkowska Dorota |
| Poland | Warsaw | Kampa Rafal Pawel |
| Poland | Warsaw | Sek Aleksandra |
| Poland | Poznan | Rybacka-Mossakowska Joanna |
| Poland | Lodz | Watala Cezary |
| Portugal | Coimbra | Pereira Susana P |
| Portugal | Porto | Bernardino RL |
| Portugal | Porto | Alves Marco G |
| Portugal | Cantanhede | Pinho Sonia A |
| Portugal | Porto | Beleza Jorge |
| Portugal | Catanhede | Pereira da Silva Grilo da Silva Filomena |
| Portugal | Coimbra | Carvalho Eugenia |
| Portugal | Porto | Carrageta D |
| Portugal | Porto | Magalhaes Jose |
| Portugal | Porto | Martins Ana Dias |
| Portugal | Porto | Crisostomo Luis |
| Portugal | Porto | Moreira Bruno P |
| Portugal | Cantanhede | Bento Guida Jose Freitas |
| Portugal | Coimbra | Palmeira Carlos |
| Portugal | Porto | Oliveira Pedro Fontes |
| Portugal | Covilhã | Dias Tania R |
| Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
| Republic of Korea | Daejeon | Park SH |
| Republic of Serbia | Belgrade | Pavlovic Kasja |
| Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
| Romania | Timisoara | Lelcu Theia |
| Romania | Timisoara | Lighezan R |
| Romania | Timisoara | Betiu AM |
| Romania | Timisoara | Avram Vlad F |
| Romania | Timisoara | Muntean Danina M |
| Romania | Timisoara | Danila Maria-Daniela |
| Russia | Moscow | Itkis YS |
| Russia | Moscow | Tsygankova P |
| Russia | Moscow | Krylova T |
| Saudi Arabia | Giza | Kotb IA |
| Serbia | Belgrad | Markovic Ivanka |
| Serbia | Belgrade | Misirlic-Dencic S |
| Serbia | Belgrade | Ivanovic Tijana |
| Serbia | Belgrade | Jeremic M |
| Serbia | Belgrade | Isakovic A |
| Serbia | Belgrade | Vukojevic M |
| Slovakia | Košice | Cizmarova Beata |
| Slovakia | Bratislava | Ukropec J |
| Slovakia | Bratislava | Ukropcova B |
| Slovakia | Bratislava | Maderova D |
| Slovakia | Bratislava | Nemec Michal |
| Slovakia | Bratislava | Sumbalova Zuzana |
| Slovenia | Ljubljana | Mars T |
| Slovenia | Ljubljana | Kopitar-Jerala Natasa |
| Slovenia | Ljubljana | Pirkmajer Sergej |
| Spain | A Coruña | Mayan MD |
| Spain | Barcelona | Zorzano Antonio |
| Spain | Sevilla | Bustos Matilde |
| Spain | Madrid | De los Rios C |
| Spain | Salamanca | Almeida Angeles |
| Spain | Barcelona | Tura Ceide O |
| Spain | Barcelona | Gama Perez Pau |
| Spain | Barcelona | Hyrossova Petra |
| Spain | Barcelona | Arago Marc |
| Spain | Armilla, Granada | Escames Germaine |
| Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
| Spain | Madrid | Cano-Abad MF |
| Spain | Barcelona | Smolders V |
| Spain | A Coruña | Blanco FJ |
| Spain | Barcelona | Gil J |
| Spain | Valencia | Victor Victor Manuel |
| Spain | Madrid | Monsalve Maria |
| Spain | Ourense | Reboredo-Rodriguez Patricia |
| Spain | Madrid | Lopez MG |
| Spain | Lleida | Gonzalo Hugo |
| Spain | Madrid | Cadenas S |
| Spain | Valencia | Borras Consuelo |
| Spain | Lleida | Nogueras L |
| Spain | Barcelona | Gonzalez Casacuberta I |
| Spain | Barcelona | Dahdah Norma Ramon |
| Spain | Valencia | Casado Pinna Marta |
| Spain | Granada | Quiles JL |
| Spain | Valencia | Fuertes Agudo Marina |
| Spain | Barcelona | Juarez D |
| Spain | Sevilla | Muntane Jordi |
| Sweden | Lund | Chamkha Imen |
| Sweden | Södertälje | Alfaro-Moreno E |
| Sweden | Lund | Simon Serrano Sonia |
| Sweden | Lund | Westerlund E |
| Sweden | Lund | Leffler M |
| Sweden | Lund | Abliva |
| Sweden | Lund | Karlsson M |
| Sweden | Linköping | Fontes-Oliveira CC |
| Sweden | Lund | Elmer Eskil |
| Sweden, SE | Lund | Aasander Frostner Eleonor |
| Switzerland | Geneva | Martinou JC |
| Switzerland | Lausanne | Zanou Nadege |
| The Netherlands | BT Amsterdam | Wuest Rob CI |
| Turkey | Tekirdag | Aral Cenk |
| Turkey | Izmir | Aydin HH |
| Turkey | Ankara | Engin Ayse Basak |
| Turkey | Ankara | Tuncay Erkan |
| Turkey | Istanbul | Baykal AT |
| USA | Philadelphia | Janowska Joanna |
| USA | St Louis | Welch R |
| USA | Iowa City | Buettner Garry R |
| USA | Fayetteville | Rao RR |
| USA | Philadelphia | Kilbaugh Todd |
| USA | Baton Rouge | Irving Brian A |
| USA | Orlando | Coen Paul M |
| USA | Fayetteville | Iyer Shilpa |
| USA | Philadelphia | Starr Jonathan |
| United Kingdom | Brighton | Moore Anthony L |
| United Kingdom | Oxford | Makrecka-Kuka Marina |
| United Kingdom | Stoke-on-Trent | McStay Gavin P |
| United Kingdom | Leicester | Moisoi Nicoleta |
| United Kingdom | Birmingham | Thakker A |
| United Kingdom | Birmingham | Joanisse S |
| Uruguay | Montevideo | Radi R |
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.
