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| {{MitoPedia | | {{MitoPedia |
| |abbr=N(PGM) | | |abbr= |
| |description=[[File:1PGM;2D;3U-.png|300px]] | | |description= |
| |info='''A''' [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-018.pdf|Bioblast pdf]] »[http://wiki.oroboros.at/index.php/File:SUIT-018.pdf Versions] | | |info='''A''' [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-018.pdf|Bioblast pdf]] »[http://wiki.oroboros.at/index.php/File:SUIT-018.pdf Versions] |
| }} | | }} |
| ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' N(PGM) | | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' |
| ::: '''[[SUIT protocol pattern]]:''' linear 1PGM;2D;3U | | ::: '''[[SUIT protocol pattern]]:''' |
| __TOC__ | | __TOC__ |
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| == References == | | == References == |
| {{#ask:[[Category:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-018]]
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| {{Template:SUIT-018}} | | {{Template:SUIT-018}} |
Revision as of 13:02, 18 January 2019
- high-resolution terminology - matching measurements at high-resolution
SUIT-018
Description
Reference: A 
»Versions
- SUIT-category:
- SUIT protocol pattern:
DLP applications
References
Steps and respiratory states
| Step
|
State
|
Pathway
|
Q-junction
|
Comment - Events (E) and Marks (M)
|
| 0DTPA
|
|
|
|
- DTPA is an iron chelator, which decreases the chemical fluorescence background created by the Amplex UltraRed assay. Administration of DTPA into the O2k-chamber is recommended before all other chemicals because the iron chelation capacity of the compound is time-dependent (approx. 10-15 min). However, the experiments can be carried out in the absence of DTPA.
|
| 0SOD
|
|
|
|
- SOD or superoxide dismutase converts the anion superoxide released by the mitochondria into H2O2, making it accessible to the Amplex UltraRed assay.
|
| 0HRP
|
|
|
|
- HRP or horseradish peroxidase catalyses the conversion of Amplex UltraRed and H2O2 towards the fluorescent resorufin.
|
| 0AmR
|
|
|
|
|
| Step
|
State
|
Pathway
|
Q-junction
|
Comment - Events (E) and Marks (M)
|
| 1GMS
|
GMSL(n)
|
NS
|
CI&CII
|
1GMS
- Respiratory stimulation by simultaneous action of type N substrates & succinate, with convergent electron flow in the NS-pathway for reconstitution of TCA cycle function.
- Non-phosphorylating resting state (LEAK state); LEAK respiration L(n) in the absence of ADP, ATP, AMP (no adenylates).
|
| 2D
|
GMSP
|
NS
|
CI&CII
|
1GMS;2D
- Respiratory stimulation by simultaneous action of type N substrates & succinate, with convergent electron flow in the NS-pathway for reconstitution of TCA cycle function.
- OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
|
| 3Ama
|
ROX
|
|
|
1GMS;2D;3Ama
- Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).
|
Strengths and limitations
Compare SUIT protocols
MitoPedia concepts:
MiP concept,
SUIT protocol,
Recommended
MitoPedia methods:
Respirometry
