Difference between revisions of "Template:SUIT-006 O2 mt D022"
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== Steps and respiratory states == | == Steps and respiratory states == | ||
[[File:| | [[File:1PGM;2D;3U-.png|400px]] | ||
{| class="wikitable" border="1" | {| class="wikitable" border="1" | ||
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|- | |- | ||
| 1SRot | | 1SRot | ||
| S<sub>L</sub> | | [[S]]<sub>L</sub> | ||
| [[S]] | | [[S]] | ||
| CII | | CII | ||
| L, LEAK respiration | | L, LEAK respiration | ||
{{Template:SUIT L n}} | {{Template:SUIT S}} {{Template:SUIT Rot}} {{Template:SUIT L n}} | ||
|- | |- | ||
| 2D | | 2D | ||
| S<sub>P</sub> | | [[S]]<sub>P</sub> | ||
| [[S]] | | [[S]] | ||
| CII | | CII | ||
| 1SRot;2D | | 1SRot;2D | ||
{{Template:SUIT OXPHOS}} | {{Template:SUIT S}} {{Template:SUIT OXPHOS}} | ||
|- | |- | ||
| 3U | | 3U | ||
| S<sub>E</sub> | | [[S]]<sub>E</sub> | ||
| [[S]] | | [[S]] | ||
| CII | | CII | ||
| 1SRot;2D;3U | | 1SRot;2D;3U | ||
{{Template:SUIT U*}} | {{Template:SUIT S}} {{Template:SUIT U*}} | ||
|- | |- |
Revision as of 16:59, 21 January 2019
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1SRot | SL | S | CII | L, LEAK respiration
Succinate, S ( type S-pathway to Q). Succinate pathway control state (S-pathway) after inhibiting CI with rotenone, which also inhibits the F-pathway. Template:SUIT L n |
2D | SP | S | CII | 1SRot;2D
Succinate, S ( type S-pathway to Q). OXPHOS capacity P (with saturating [ADP]), active OXPHOS state. |
3U | SE | S | CII | 1SRot;2D;3U
Succinate, S ( type S-pathway to Q). Uncoupler titration (avoiding inhibition by high uncoupler concentrations) to obtain electron transfer (ET) capacity E (noncoupled ET-state). Test for limitation of OXPHOS capacity P by the phosphorylation system (ANT, ATP synthase, phosphate transporter) relative to ET capacity E in mt-preparations: E-P control efficiency and E-L coupling efficiency. In living cells: E-R control efficiency and E-L coupling efficiency. |
4Ama | ROX | Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt). |
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary