From Bioblast
| Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenerg Commun 2021.1. https://doi.org/10.26124/bec:2021-0001 |
» Bioenerg Commun 2021.1. 
published online 2021-06-30
Cardoso Luiza HD, Doerrier Carolina, Gnaiger Erich (2021) Bioenerg Commun
Abstract: 
doi:10.26124/bec:2021-0001
For the advanced study of mitochondrial function, high-resolution respirometry is extended by fluorometric measurement of ATP production using the fluorophore Magnesium Green™ (MgG). A common problem with several fluorescent dyes is the inhibition of mitochondrial respiration. In the present study, a coupling control protocol was applied in combination with MgG to measure ATP production simultaneously with respiration for calculation of P»/O2 ratios. MgG at 1.1 µM did not affect respiration through the NADH-linked and succinate-linked pathways. Respiration was not inhibited in any of the coupling control states, hence coupling control efficiencies were not affected by MgG.
• Keywords: ATP, ATP production, high-resolution respirometry, Magnesium Green, mitochondria, oxidative phosphorylation, fluorometry, FluoRespirometry
• Bioblast editor: Gnaiger E
• O2k-Network Lab: AT Innsbruck Oroboros
ORCID: 
Cardoso Luiza HD, Doerrier Carolina, Gnaiger Erich
Data availability
- Original files are available Open Access at Zenodo repository: 10.5281/zenodo.4916141
Support
- Supported by project NextGen-O2k which has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 859770. An initiative of the MitoEAGLE Task Group of the Mitochondrial Physiology Society.
Keywords
- Bioblast links: ATP production - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
Phosphorylation pathway
- Phosphorylation pathway substrates
- » ADP
- » ATP
- » Inorganic phosphate
- Phosphorylation pathway substrates
- Phosphorylation pathway inhibitors
Coupling control
Respiratory complexes and coupling
ATP production measurement
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Cited by
- Gnaiger E (2021) Beyond counting papers – a mission and vision for scientific publication. Bioenerg Commun 2021.5. https://doi:10.26124/BEC:2021-0005
Preprint
Labels: MiParea: Respiration, Instruments;methods
Organism: Mouse
Tissue;cell: Heart
Preparation: Isolated mitochondria
Regulation: ATP production, Coupling efficiency;uncoupling Coupling state: LEAK, OXPHOS, ET Pathway: N, S, ROX HRR: Oxygraph-2k, O2k-Fluorometer, NextGen-O2k, O2k-Protocol
SUIT-006, MgG, MitoEAGLEPublication, BEC2021.5, MitoFit 2022 NADH, O2k-Demo, ATP
