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| [[File:Bioblast alert.jpg|240px|right]] | | {{Bioblast header page name}} |
| | [[Image:BB-Bioblast.jpg|left|30px|link=Bioblast:About|Bioblast wiki]] |
| | [[File:Bioblast alert.jpg|240px|right|link=Bioblast_alert|Bioblast alert]] |
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| '''Bioblast alert''' is prepared by the [[Bioblast editorial board]] to draw attention to specific topics on the '''Bioblast wiki'''. | | '''[[Bioblast alert|back to "Bioblast alert" main page]]''' |
| '''Bioblast alert''': for information purposes, not intended to be an advertisement.
| | __TOC__ |
| Contributions are welcome.
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| === [[Bioblast alert|Bioblast alert]] 2012(06): 2012-07-20 === | | === Bioblast alert 2015(01): 2015-01-01 === |
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| * '''MitoPedia news''': [[Myxothiazol]] is available again, ''but what about'' [[glycerophosphate|α-glycerophosphate]] ?
| | [[Image:BB-Bioblast.jpg|left|30px|link=Gentle Science|Gentle Science]] |
| :: >> More information on the '''MitoPedia list''' [[List of substrates and metabolites|'''Substrates and metabolites''']]
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| * '''MitoPedia terminology''': [[State 3]] - is [ADP] saturating? ''Not generally, but certainly in the'' [[OXPHOS capacity|OXPHOS state]] (''P'').
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| :: >> Update on the '''MitoPedia glossary:''' [[Glossary: Respiratory states|'''Respiratory states''']]
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| * '''Hypoxia and high altitude''': [[CH Zurich Lundby C]], [[DK Copenhagen Boushel RC]], [[AT Innsbruck Gnaiger E]]
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| :* [[Jacobs 2012 Exp Physiol|Jacobs RA, Boushel RC, Wright-Paradis C, Calbet JA, Robach P, Gnaiger E, Lundby C (2012) Mitochondrial function in human skeletal muscle following high altitude exposure. Exp Physiol [Epub ahead of print].]]
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| :* [[Robach 2012 Br J Sports Med|Robach P, Siebenmann C, Jacobs RA, Rasmussen P, Nordsborg N, Pesta D, Gnaiger E, Díaz V, Christ A, Fiedler J, Crivelli N, Secher NH, Pichon A, Maggiorini M, Lundby C (2012) The role of haemoglobin mass on VO<sub>2max</sub> following normobaric 'live high-train low' in endurance-trained athletes. Br J Sports Med Jul 12. [Epub ahead of print].]]
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| :* [[Pesta 2011 Am J Physiol Regul Integr Comp Physiol|Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301: R1078–R1087.]]
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| :* [[Scandurra 2010 Adv Exp Med Biol|Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: Facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662: 7-25.]]
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| | [[Gentle_Science#Bioenergetics_-_Arts_meets_Gentle_Science |'''Bioenergetics - Arts meets Gentle Science''']] - Bioenergetics Exhibition, March 15-June 17, 2015, Science Museum of Virginia, US - a Gentle Science project initiated by [[Iyer S |Shilpa Iyer]]. |
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| === Bioblast alert 2012(05): 2012-06-05 ===
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| * '''MitoPedia topic''': '''''Mitochondria and Genetics''''' - '''''[[Peroxisome proliferator-activated receptor gamma coactivator 1-alpha|the Coactivator PGC-1α]]'''''
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| * '''Bioblast announcement''': [http://www.umdf.org/site/c.piKYL1PHLtF/b.4864979/k.C003/Home.htm UMDF: Mitochondrial Medicine 2012] - 2012-June-13 to 16 - Capitol Hill, Bethesda, Washington DC, USA
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| * '''Comparison of common bioenergetic analysis methods''': [[Zhang_2012_Nat Protoc|Zhang J, Nuebel E, Wisidagama DR, Setoguchi K, Hong JS, Van Horn CM, Imam SS, Vergnes L, Malone CS, Koehler CM, Teitell MA (2012) Measuring energy metabolism in cultured cells, including human pluripotent stem cells and differentiated cells Nat Protoc 7: 1068-1085.]] >> ''Continue the [[O2k_versus_multiwell_respirometer|discussion]]''
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| == Bioblast alert 2012(04): 2012-04-29 ==
| | [[Image:Logo-MiP2014.jpg|left|55px|link=MiP2015|MiPArt by Odra Noel]] |
| * '''MitoPedia topic''': '''''Mitochondria''''' - can we decide on a common abbreviation? - or simply call them '''''[[Bioblasts]]'''''. --> '''[[Mitochondria]]''' and [[Talk:Mitochondria]]
| | » '''MiP''schools'' 2015''': »» [[MiPschool Cape Town 2015 |'''Cape Town ZA, March 24-28''']] »» [[MiPschool London 2015 |'''London UK, April 20-25''']] »» [[MiPschool Greenville 2015 |'''Greenville US, August 10-14''']]. |
| * '''Bioblast news''': '''Light in the powerhouse of the cells''' - [http://www.oroboros.at/?Houska-Award_2011 a mt-project is the winner of the Houska Award 2011].
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| * '''Comparative mt-physiology - life style and training:'''
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| ::[[Votion 2012 PLoS One|Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7(4): e34890.]]
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| == Bioblast alert 2012(03): 2012-03-23 / 2012-04-03 ==
| | » [[MiP2015 |'''Mitochondrial Physiology Conference MiP2015, Sep 7-11''']], Luční Bouda, Czech Republic. |
| '''MitoPedia topic''': '''[[Permeabilized_muscle_fibres#Oxygen_dependence_of_ROS_production_-_are_permeabilized_fibres_a_valid_model.3F|Oxygen dependence of ROS production - are permeabilized fibres a valid model?]]''' | |
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| --> [[Talk:Permeabilized muscle fibres|'''''Discussion''''']]
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| '''''Drosophila'' vs. sandworm: High-resolution respirometry in permeabilized muscle fibres'''
| | [[Image:BB-Bioblast.jpg|20px|link=Bioblast:About|Bioblast wiki]] '''[[Bioblast track]]''' - evolving hot Bioblast topics: reflections at the beginning of the New Year. |
| # [[Pichaud 2012 Comp Biochem Physiol A Mol Integr Physiol|Pichaud N, Rioux P, Blier PU (2012) In situ quantification of mitochondrial respiration in permeabilized fibers of a marine invertebrate with low aerobic capacity. Comp Biochem Physiol A Mol Integr Physiol 161: 429-435.]]
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| # [[Pichaud 2011 Am J Physiol Regul Integr Comp Physiol|Pichaud N, Ballard JWO, Tanguay RM, Blier PU (2011) Thermal sensitivity of mitochondrial functions in permeabilized muscle fibers from two populations of Drosophila simulans with divergent mitotypes. Am J Physiol Regul Integr Comp Physiol 301: R48-R49.]]
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| ''New from the Ballard lab'': | |
| * [[Horan 2012 J Gerontol A Biol Sci Med Sci|Horan MP, Pichaud N, Ballard JW (2012) Review: Quantifying mitochondrial dysfunction in complex diseases of aging. J Gerontol A Biol Sci Med Sci [Epub ahead of print].]]
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| == Bioblast alert 2012(02): 2012-03-06 ==
| | '''<big><big><big>HRR</big></big></big> - high-resolution respirometry''' was established 20 years ago: [[Gnaiger 1995 J Bioenerg Biomembr |Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 1995.]] |
| * '''50 Years ago''': '''Nick Holonyak''', considered as the "father of the light-emitting diode", developed the first practical '''visible-spectrum LED in 1962'''. ''Which colour? What is a'' '''LED'''''?'' >>> see '''[[Light emitting diode|Glossary: Fluorescence]]'''
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| '''Mitochondrial dys/function in obesity vs. anorexia''':
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| # [[Lindfors 2011 Proc Natl Acad Sci U S A|Lindfors C, Nilsson IA, Garcia-Roves PM, Zuberi AR, Karimi M, Donahue LR, Roopenian DC, Mulder J, Uhlén M, Ekström TJ, Davisson MT, Hökfelt TG, Schalling M, Johansen JE (2011) Hypothalamic mitochondrial dysfunction associated with anorexia in the anx/anx mouse. Proc Natl Acad Sci U S A 108: 18108-18113.]]
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| # [[Holmstroem 2012 Am J Physiol Endocrinol Metab|Holmstroem MH, Iglesias-Gutierrez E, Zierath JR, Garcia-Roves PM (2012) Tissue-specific control of mitochondrial respiration in obesity-related insulin resistance and diabetes. Am J Physiol Endocrinol Metab 302: E731-E739.]]
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| == Bioblast alert 2012(01): 2012-02-27 == | | === Bioblast alert 2015(02): 2015-04-28 === |
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| Two exciting publications on '''mitochondrial [[p50|''p''<sub>50</sub>]]''', apparent [[excess capacity]] of cytochrome c oxidase, '''[[basal metabolic rate]]''', [[P/O ratio]]s and ergometric [[efficiency]]:
| | * Gentle Science: The '''Vancouver Protocol''' carries the spirit of Gentle Science. Do involved editors, authors and peer reviewers know that? »[[Gentle_Science#The_Vancouver_Protocol.2C_ICMJE.2C_and_Gentle_Science |''more details'']] |
| # [[Larsen_2011_FASEB_J|Larsen FJ, Schiffer TA, Sahlin K, Ekblom B, Weitzberg E, Lundberg JO (2011) Mitochondrial oxygen affinity predicts basal metabolic rate in humans. FASEB J 25: 2843-2852.]]
| | * [[MitoGlobal Events]]: '''[[UMDF2015 |Mitochondrial Medicine, UMDF2015]]: Washington DC US, 2015 Jun 17-20'''. |
| # [[Larsen_2011_Cell_Metab|Larsen FJ, Schiffer TA, Borniquel S, Sahlin K, Ekblom B, Lundberg JO, Weitzberg E (2011) Dietary inorganic nitrate improves mitochondrial efficiency in humans. Cell Metab 13: 149-159.]]
| | * [[MitoGlobal Organizations]]: Amy Goldstein ([[Mitochondrial_Medicine_Society|Mitochondrial Medicine Society]]) created the '''Mitochondrial Global Networks map'''. »[http://www.mitosoc.org/clinics ''see the map'']. |
| | * Weissig V, Edeas M, eds (2015) Mitochondrial medicine: probing mitochondrial function. [[Weissig_2015_Methods_Mol_Biol_I |Methods Mol Biol 1264:1-480]]. |
| | * MitoPedia: '''What is wrong with the RCR?''' »''Find out:'' [[Image:j--P.jpg|50 px|link=OXPHOS-coupling efficiency |OXPHOS-coupling efficiency]] [[OXPHOS-coupling efficiency]] |
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| == Bioblast alert 2011(04): 2011-12-24 == | | === Bioblast alert 2015(03): 2015-07-03 === |
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| * '''Congratulations''' to [[Pesta D|Dr. Dominik Pesta]], for receiving his degree of a PhD at the Medical University of Innsbruck! (Jan 2012)
| | [[Image:MitoPedia.jpg|40px|link=MitoPedia|MitoPedia]] » '''[[Melatonin |Melatonin beyond the clock]]''' |
| * [[Bioblast editorial board]] - [[Talk:Bioblast editorial board|Why a wiki editorial board?]]
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| * '''[[SUIT protocol]]s for HRR: from intact cells to permeabilized muscle fibres''':
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| ::[[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.]]
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| | [[Image:MitoPedia.jpg|40px|link=MitoPedia|MitoPedia]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] - [[Image:L.jpg|link=LEAK respiration|LEAK]] - [[Image:E.jpg|link=ET capacity|ETS]] - [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]: this is the 'phosphorylation control protocol' (PCP) for intact cells - or better '''[[coupling-control protocol]]''' (CCP)? |
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| == Bioblast alert 2011(03): 2011-11-24 ==
| | » Special Issue on [[ Media:Mitochondrial_Biology_Toxicology_and_Medicine.pdf | Mitochondrial Biology, Toxicology, and Medicine]] by Ryan J. Mailloux, University of Ottawa, Ottawa, Canada ( [mailto: [email protected] rmaillou@uottawa. ca] ) - deadline August 14, 2015. |
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| * '''Fluorometry combined with high-resolution respirometry''': Light guide inserted into the chamber through a black PEEK stopper<ref>[[Anderson 2011 Am J Physiol Heart Circ Physiol|Anderson EJ, Rodriguez E, Anderson CA, Thayne K, Chitwood WR, Kypson AP (2011) Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways. Am J Physiol Heart Circ Physiol 300: H118-124.]]</ref>, or optical probe operated through the O2k-glass window<ref>[[Hickey 2012 J Comp Physiol B|Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (''Hemiscyllum ocellatum''). J Comp Physiol B [Epub ahead of print] ]]</ref>.
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| *'''[[Talk:Fluorometry|Fluorometry versus fluorimetry]]''' - term of the week. >>>New '''[[Glossary: Fluorometry]]''' - high-resolution terminology.
| | » [[MitoGlobal Events]]: [[MiP2015]] - early registration deadline July 14! |
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| == Bioblast alert 2011(02): 2011-11-17 == | | === Bioblast alert 2015(04): 2015-08-24 === |
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| * '''[http://www.oroboros.at/index.php?ioc64 O2k-Workshop IOC64 - Centre for Cellular and Molecular Biology, Hyderabad, India]: 2011-12-08'''.
| | [[Image:Logo-MiP2014.jpg|40px|link=MiP2015|MiPArt by Odra Noel]] [[MitoGlobal Events]]: Programme '''Mitochondrial Physiology Conference, CZ, Sept 2015''' now online: '''»[[MiP2015]]«'''. Last-minute registrations are encouraged - a special opportunity for MiP''members'', students and young investigators - no surcharge for late registration. |
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| * '''Heart failure and mitochondrial dysfunction'''. Increased [[OXPHOS capacity]] after cold preservation of the human heart – a paradox resolved by a dyscoupling mechanism.
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| ::[[Lemieux 2011 Int J Biochem Cell Biol|Lemieux H, Semsroth S, Antretter H, Hoefer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43: 1729–1738.]] --> [[Talk:Lemieux 2011 Int J Biochem Cell Biol|Discussion: Complementary results on the PGC-1a pathway]].
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| * '''[[Tissue storage]]''' on ice is possible for limited periods of time (several hours) with preservation of mitochondrial respiratory function, as evaluated with [[SUIT protocol]]s. ([[Scientific service]])
| | [[Image:BB-Bioblast.jpg|30px|link=Bioblast:About|Bioblast wiki]] Send your favourite '''» [[Bioblast picture of the month]]'''. |
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| * '''[[Uncoupled]], [[non-coupled]], [[dyscoupled]]''' - term of the week: a hallmark of high-resolution respirometry is a matching high-resolution terminology.
| | [[Image:MitoGlobal.jpg|40px|link=Mitochondrial_Global_Network|MitoGlobal]] 5 years ago: the '''Bioblast Boost''' » [[Bioblast:About]] |
| ::[[Talk:Uncoupled respiration|Evaluate an example of low-resolution terminology]]: '''''[[Dyscoupled respiration|Uncoupling]]''' was studied in stressed cells by measurement of coupled respiration, evaluation of '''[[LEAK|uncoupled]]''' respiration after inhibition with oligomycin, and FCCP titration to quantify '''[[non-coupled|uncoupled]]''' respiration''.
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| | [[Image:MitoPedia.jpg|40px|link=MitoPedia|MitoPedia]] » [[MitoPedia: Respiratory states]]: apparent excess capacities of respiration [[Image:ExP.jpg|60 px|link=Excess E-P capacity|Excess ''E-P'' capacity]] [[Image:ExR.jpg|60 px|link=Excess E-R capacity|Excess ''E-R'' capacity]] |
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| == Bioblast alert 2011(01): 2011-11-11 ==
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| * A key question on the separation of '''qualitative versus quantitative change of mitochondrial function''' is addressed in a new O2k-Publication. Does training induce mild uncoupling? Are sedentary humans healthy controls?
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| ::[[Pesta_2011_Am J Physiol Regul Integr Comp Physiol|Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301: R1078–R1087.]]
| | == Bioblast alert - previous issues == |
| * [[ETS capacity]] plays a key role in such analyses, which cannot be achieved quantitatively without careful '''[[Optimum uncoupler concentration|uncoupler titrations]]'''. ([[Scientific service]]) | | * [[Bioblast alert 2015]] |
| * '''[[Basal respiration]]''' - term of the month: a hallmark of high-resolution respirometry is a matching high-resolution terminology. | | * [[Bioblast alert 2014]] |
| | * [[Bioblast alert 2013]] |
| | * [[Bioblast alert 2012]] |
| | * [[Bioblast alert 2011]] |
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| | [[Category:Gentle Science]] |
Bioblast alert 2015
Bioblast track - evolving hot Bioblast topics: reflections at the beginning of the New Year.
OXPHOS-coupling efficiency
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: this is the 'phosphorylation control protocol' (PCP) for intact cells - or better coupling-control protocol (CCP)?
MitoGlobal Events: Programme Mitochondrial Physiology Conference, CZ, Sept 2015 now online: »MiP2015«. Last-minute registrations are encouraged - a special opportunity for MiPmembers, students and young investigators - no surcharge for late registration.
5 years ago: the Bioblast Boost » Bioblast:About
