MiPNet23.01 IOC126 Innsbruck AT: Difference between revisions
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|Hands-on: Assembly and maintenance of TPP and reference electrode O2k-MultiSensor-Manual MiPNet15.03 | |Hands-on: Assembly and maintenance of TPP and reference electrode [[MiPNet15.03_O2k-MultiSensor-ISE O2k-MultiSensor-Manual|MiPNet15.03]] | ||
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|Hands-on: Set up of the instruments with TPP and reference electrodes + TPP calibration O2k-MultiSensor-Manual MiPNet15.03 | |Hands-on: Set up of the instruments with TPP and reference electrodes + TPP calibration [[MiPNet15.03_O2k-MultiSensor-ISE O2k-MultiSensor-Manual|MiPNet15.03]] | ||
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Revision as of 16:48, 11 December 2017
MiPNet23.01 IOC126 Innsbruck AT
Innsbruck AT, 2018 Jan 24-26. Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. IOC126. |
Oroboros (2018-01-24) Mitochondr Physiol Network
Abstract:
Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. Innsbruck, Austria; 2018 Jan 24-26.
β’ O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit
General information
- The Oroboros O2k-Workshop is an advanced course which covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (isolated mitochondria, homogenate) with potentiometric (TPP+/O2k-TPP+ ISE-Module) and amperometric method (TMRM, safranin/O2k-Fluo LED2-Module) Furthermore, data analysis - from TPP+ to mt-membrane potential - constitutes a major topic.
Venue
- Information on travel and venue:IOC Innsbruck.
- Β» Hotel suggestion: Hotel Weisses Kreuz
- Mention "Oroboros" when booking in order to receive a discount.
Programme
Wednesday 24th Topic Room 09:00 Get-together: Introduction of participants and their research interests MiPArt 09:30 O2k-MultiSensor overview, DatLab7, DatLab7 Protocols MiPArt 10:00 Coffee/Tea 10:30 Determination of mitochondrial (mt) membrane potential with potentiometric TPP+/O2k-TPP+ ISE-Module - Introduction MiPArt 11:15 Hands-on: Assembly and maintenance of TPP and reference electrode MiPNet15.03 MiPArt 12:30 Lunch break 14:00 Hands-on: Set up of the instruments with TPP and reference electrodes + TPP calibration MiPNet15.03 MitoFit ORO Lab 12:30 Coffee/Tea 16:30 Hands-on continued: TPP chemical background MiPNet14.05 MiPArt
Thursday 25th | Topic | Room |
09:00 | Introduction in Planning and Performing a TPP experiment; Instrumental background oxygen flux in the presence of the TPP electrode O2k-Background MiPNet14.06 Instrumental O2 background | MiPArt |
09:30 | Hands-on: Calibration of TPP electrode | MitoFit ORO Lab |
10:30 | Coffee/Tea | |
11:00 | Hands-on continue TPP: experiment with mouse brain mitochondria | MitoFit ORO Lab |
12:30 | Lunch break | |
14:00 | From the TPP signal to mt-membrane potential - Guide through the Excel templates. MiPNet14.05 mathematics | MiPArt |
14:30 | Analysis of TPP experiment - discussion on Mitochondrial membrane potential, MiPNet14.05 mathematics | MiPArt |
16:00 | Coffee/Tea | |
16:30 | Hands-on: O2k setup for air calibration | MitoFit ORO Lab |
17:00 | Determination of mitochondrial (mt) membrane potential with amperometric method (TMRM, safranin/O2k-Fluo LED2-Module) - introduction | MitoFit ORO Lab |
17:30 | Hands-on: Set up of the instruments with LED2-Module, LED2-Module MiPNet17.05,
Calibration of Safranin/TMRM, chemical background experiment |
MitoFit ORO Lab |
Friday 26th | Topic | Room |
09:00 | Introduction in planning and performing an experiment with Safranin/TMRM | MiPArt |
09:30 | Hands-on: O2k setup for air calibration | MitoFit ORO Lab |
10:30 | Coffee'/Tea | |
11:00 | Hands-on: Calibration of Safranin/TMRM, experiment with HEK cells | MitoFit ORO Lab |
12:30 | Lunch break | |
14:00 | Analysis of TMRM/Safranin experiment - discussion | MiPArt |
15:00 | Coffee/Tea | |
16:30 | Round table: questions | MiPArt |
17:00 | Other potentiometric, amperometric, and fluorometric O2k-MultiSensor applications, The Bioblast wiki, O2k-Network and MitoEAGLE, feedback and conclusions | MiPArt |
18:00 | Farewell activity |
Registration
Tutors and Lecturers
Tutors
Sumbalova Zuzana, PhD, Medical University of Innsbruck - Principal investigator
Chang Shao-Chiang MSc., Oroboros Instruments, Medical University of Innsbruck - PhD. student TRACT
Passrugger Manuela, BSc., Oroboros Instruments - Biomedical Assistant
Ana Carolina Bastos Sant'Anna Silva, MSc., Oroboros Instruments, Medical University of Innsbruck - PhD. student TRANSMIT
Lecturers and assistance
Doerrier Carolina,PhD, Oroboros Instruments - Chief Scientific Officer
Komlodi Timea,Dr., Oroboros Instruments - Scientific Researcher
Meszaros Andras, MD, PhD, Oroboros Instruments - Postdoctoral researcher
List of participants
Participant | Institution | |
---|---|---|
Kandel Sunil Mani | US WI Milwaukee Dash RK - Medical College of Wisconsin | |
Smirnova Irina | SE Stockholm Smirnova I - Stockholm University | |
Labieniec-Watala Magdalena | PL Lodz Watala C - University of Lodz | |
Janssen Connie | NL Leiden Lindeman JH - Leiden University Medical Center | |
de Kok MichΓ©le | NL Leiden Lindeman JH - Leiden University Medical Center | |
Steffen Anja | NL Leiden Lindeman JH - Leiden University Medical Center | |
Siewiera Karolina | PL Lodz Watala C - University of Lodz | |
Michalak Slawomir | PL Poznan Michalak S - Poznan University of Medical Sciences | |
Fernandez-Vizarra Erika | UK Cambridge Zeviani M - University of Cambridge |
Contact
- Oroboros Instruments
- High-Resolution FluoRespirometry
- SchΓΆpfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and cell research
IOC recommended reading
Project support
Labels:
ORO, IOC, MitoFit, Next, 2018
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.