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Difference between revisions of "MiPNet20.10 IOC106 Schroecken"

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{{Publication
{{Publication
|title=[[Image:OROBOROS-solo.png|right|60px|link=OROBOROS INSTRUMENTS]]
|title=[[Image:OROBOROS-solo.png|right|60px|link=Oroboros Instruments]]
'''Schroecken AT''', 2015 Oct 06-11. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, '''IOC106.''' [[Media:MiPNet20.10 IOC106 2015-10 Schroecken.pdf|»Bioblast pdf«]]
'''Schroecken AT''', 2015 Oct 06-11. Oroboros O2k-Workshop on HRR and O2k-Fluorometry, '''IOC106.'''  
|authors=OROBOROS
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/e/e0/MiPNet20.10_IOC106_2015-10_Schroecken.pdf |Bioblast pdf]]  
|authors=Oroboros
|year=2015-10-06
|year=2015-10-06
|journal=Mitochondr Physiol Network
|journal=Mitochondr Physiol Network
|abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops]]
|abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops]]
'''OROBOROS O2k-Workshop on HRR and O2k-Fluorometry'''. Schroecken, Vorarlberg, Austria; 2015 Oct 06-11.
'''Oroboros O2k-Workshop on HRR and O2k-Fluorometry'''. Schroecken, Vorarlberg, Austria; 2015 Oct 06-11.
|mipnetlab=AT_Innsbruck_OROBOROS
|mipnetlab=AT_Innsbruck_Oroboros
|mammals_and_models=
|mammals_and_models=
}}
}}
{{Labeling
{{Labeling
|additional=ORO, IOC, 2015, Next, MitoFit
|instruments=Oxygraph-2k
|additional=ORO, IOC, 2015, MitoFit
|mammals_and_models=
|mammals_and_models=
}}
}}
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* ''Thank you for the excellent seminar and your knowledgeable staff'' - '''[[Coker R| Robert Coker]]''', University of Alaska, Institute of Arctic Biology (US)
* ''Thank you for the excellent seminar and your knowledgeable staff'' - '''[[Coker R| Robert Coker]]''', University of Alaska, Institute of Arctic Biology (US)
== Programme ==
:» [[Media:MiPNet20.10 IOC106 2015-10 Schroecken.pdf |IOC106 Programme]]
== General information ==
This O2k-Workshop provides a detailed and practical introduction to the O2k and the O2k-Fluorometer, with real-time  analysis by DatLab and applications of the TIP2k. Demo experiments  illustrate the principle and show the unique advantages and limitations  of simultaneous monitoring of oxygen concentration, respiration,  hydrogen peroxide production or mt-membrane potential.
:» Information on travel and venue: '''[[IOC Schroecken]]'''.
== Registration ==
'''''FULLY BOOKED'''''
Please fill in the registration form and send it to [mailto:[email protected]| [email protected]]
'''Early Bird registration deadline:''' August 31 


   
   
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[[File:DoerrierC.JPG|80px|Carolina Doerrier]]
[[File:DoerrierC.JPG|80px|Carolina Doerrier]]
[[Doerrier Carolina]], Mitochondrial Application Specialist and PostDoc, OROBOROS INSTRUMENTS
[[Doerrier Carolina]], Mitochondrial Application Specialist and PostDoc, Oroboros Instruments


[[File:Gnaiger Erich.jpg|80px|Erich Gnaiger]]
[[File:Gnaiger Erich.jpg|80px|Erich Gnaiger]]
[[Gnaiger Erich]], CEO, OROBOROS INSTRUMENTS
[[Gnaiger Erich]], CEO, Oroboros Instruments


[[File:HoppelF.jpg|80px|Florian Hoppel]]
[[File:HoppelF.jpg|80px|Florian Hoppel]]
[[Hoppel Florian]], PhD Student, OROBOROS INSTRUMENTS
[[Hoppel Florian]], PhD Student, Oroboros Instruments


[[File:VG.jpg|80px|Laner Verena]]
[[File:VG.jpg|80px|Laner Verena]]
[[Laner Verena]], Chief Operating Officer (COO), OROBOROS INSTRUMENTS
[[Laner Verena]], Chief Operating Officer (COO), Oroboros Instruments


[[File:Meagan McManus.png|80px| Meagan McManus]]
[[File:Meagan McManus.png|80px| Meagan McManus]]
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|-
|-
| [[Ball Darran]]
| [[Ball Darran]]
| '''[[UK Southampton Grocott MP]]:''' University of Southampton, Academic Unit of Cancer Sciences (UK)  
| '''[[UK Southampton Grocott MPW]]:''' University of Southampton, Academic Unit of Cancer Sciences (UK)  
|-
|-
| [[Bezuidenhout Nicole]]
| [[Bezuidenhout Nicole]]
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|-
|-
| [[Droescher Stephanie]]
| [[Droescher Stephanie]]
| '''[[AT Innsbruck OROBOROS]]:''' OROBOROS INSTRUMENTS (AT)
| '''[[AT Innsbruck Oroboros ]]:''' Oroboros Instruments (AT)
|-
|-
| [[Einer Claudia]]
| [[Einer Claudia]]
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|-
|-
| [[Sharaf Mahmoud]]
| [[Sharaf Mahmoud]]
| '''CA Charlottetown Kamunde C:''' University of Prince Edward Island, Department of Biomedical Sciences (CA)
| '''[[CA Charlottetown Kamunde C]]:''' University of Prince Edward Island, Department of Biomedical Sciences (CA)
|-
|-
| [[Smith James]]
| [[Smith James]]
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|-
|-
| [[Ward Marie Louise]]
| [[Ward Marie Louise]]
| '''NZ Auckland Ward ML:''' The University of Auckland, Department of Physiology, Faculty of Medical and Health Sciences (NZ)
| '''[[NZ Auckland Ward ML]]:''' The University of Auckland, Department of Physiology, Faculty of Medical and Health Sciences (NZ)
|-
|-
| [[Weiss Alexander]]
| [[Weiss Alexander]]
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|-
|-
| [[West Malcolm]]
| [[West Malcolm]]
| '''[[UK Southampton Grocott MP]]:''' University of Southampton, Academic Unit of Cancer Sciences (UK)  
| '''[[UK Southampton Grocott MPW]]:''' University of Southampton, Academic Unit of Cancer Sciences (UK)  
|}
|}
==IOC106 results ==
=== O2k-Demo experiment on fish heart homogenate (Shredder)===
* [[Media:2015-10-09 P1-02.DLD|2015-10-09 P1-02.DLD]] 
* [[Media:IOC106 O2k Shredder demo dilution.xls|IOC106 O2k Shredder demo dilution.xls]]
=== O2k-Demo experiment on intact cells ===
* [[Media:2015-10-08 P1-02.DLD|2015-10-08 P1-02.DLD]]
* [[Media:IOC106demo O2k Intact cells.xls|IOC106demo O2k Intact cells.xls]]   
=== Demo analysis of hands-on experiments on permeabilized cells ===
* [[Media:2015-10-08 P6-03.DLD|2015-10-08 P6-03.DLD]]
* [[Media:Demo analysis HRR&H2O2.xls|Demo analysis HRR&H2O2.xls]]
* [[Media:SUIT_perm_cells - H2O2.pdf|SUIT perm cells - H2O2.pdf]] 
== Impressions from the IOC106 ==
<gallery mode=default widths="300px" heights="300px" perrow=4>
Image:IOC106 (1).jpg|Hands-on experiment: Robert Coker, Carina Lindquist and Verena Laner.
Image:IOC106 (2).jpg|Eija Pirinen.
Image:IOC106 (4).jpg|In Action: Meagan McManus.
Image:IOC106 (5).jpg|Anthony J. Molina, Giorgia Lamberti and Peter Overby.
Image:IOC106 (9).jpg|Clarissa Haas working experimentally.
Image:IOC106 (10).jpg|Marie-Luise Ward, Darran Ball and Malcolm West.
Image:IOC106 (11).jpg|Demonstration of the POS-Service by Carolina Doerrier with Chiara Volani, Allan Hanson and Giorgia Lamberti.
Image:IOC106 (12).jpg|Are there any bubbles? Mahmoud Sharaf, Junichi Matsumot and Giselli Scaini.
Image:IOC106 (13).jpg|Claudia Einer, Alexander Weiss and Sabine Schmitt doing the POS-Service.
Image:IOC106 (14).jpg|Working in groups - running different experiments on 7 O2k.
Image:IOC106 (15).jpg|Discussions with Erich about protocols.
Image:IOC106 (17).jpg|Waiting for stabilization of the flux.
Image:IOC106 (18).jpg|Discussing some of the results with Erich.
Image:IOC106 (20).jpg|Florian Hoppel ‘helping-out’
Image:IOC106 (22).jpg|Data analysis by … and Chiara Volani
Image:IOC106 (24).jpg|Giselli Scaini presenting her work.
Image:IOC106 (25).jpg|Mahmoud Sharaf giving an overview of his research.
Image:IOC106 (26).jpg|Sunny weather in Schroecken – use it for a group picture.
Image:IOC106 (6).jpg|On the walk to the Alpmuseum …
Image:IOC106 (7).jpg|Having fun and trying to jump as high as possible for the picture.
Image:IOC106 (8).jpg|Group picture with Edwin (Alpmuseum’s director) in the front.
Image:IOC106 (27).jpg|You can never have enough good group pictures.
Image:IOC106 (28).jpg|Anthony Molina in the lead.
Image:IOC106 (29).jpg|The view to the hotel on the walk to the Alpmuseum.
Image:IOC106 (31).jpg|Vegetation changes already its colour. James Smith in the lead.
Image:IOC106 (37).jpg|Having a break.
Image:IOC106 (38).jpg|Claudia Einer, Carolina Doerrier, Sabine Schmitt and Carine Lindquist.
Image:IOC106 (39).jpg|Science and hiking collaboration: Anthony Molina and Zuzana Sumbalova.
Image:IOC106 (40).jpg|Enjoying the view.
Image:IOC106 (42).jpg|Chiara Volani and Daniel Tyrrell in the front …
Image:IOC106 (43).jpg|Not everybody is fully concentrated on the walk…
Image:IOC106 (44).jpg|A well-deserved break in the ‘Auenfelder Hütte’.
Image:IOC106 (45).jpg|A toast on a successful O2k-Workshop.
Image:IOC106 (46).jpg|The ORO-Team: Verena, Erich, Caro (left to right).
Image:IOC106 (48).jpg|Stopping for a picture is necessary in this picturesque-environment.
Image:IOC106 (49).jpg|Zuzana Sumbalova and Clarissa Haas.
Image:IOC106 (51).jpg|Erich and Edwin
Image:IOC106 (52).jpg|Live demonstration how to make wool by Anna.
Image:IOC106 (53).jpg|Guide Edwin is telling stories of the past.
Image:IOC106 (55).jpg|Robert Coker and Anna.
</gallery>




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== Contact ==
== IOC recommended reading ==
:»  [[IOC recommended reading |More details]]
: '''» [[MitoPedia: Respiratory states]]''' [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]]  [[Image:E.jpg|link=ET capacity|ETS]] [[Image:L.jpg|link=LEAK  respiration|LEAK]] [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]


:» [[OROBOROS_Contact |OROBOROS INSTRUMENTS]]


== Impressions from the IOC106 ==
== General information ==


<gallery mode=default perrow=4 widths="300px" heights="300px">
This O2k-Workshop provides a detailed and practical introduction to the O2k and the O2k-Fluorometer, with real-time  analysis by DatLab and applications of the TIP2k. Demo experiments  illustrate the principle and show the unique advantages and limitations  of simultaneous monitoring of oxygen concentration, respiration,  hydrogen peroxide production or mt-membrane potential.  
Image:IOC106 (1).jpg
:» Information on travel and venue: '''[[IOC Schroecken]]'''.
Image:IOC106 (2).jpg
Image:IOC106 (4).jpg
Image:IOC106 (5).jpg
Image:IOC106 (9).jpg
Image:IOC106 (10).jpg
Image:IOC106 (11).jpg
Image:IOC106 (12).jpg
Image:IOC106 (13).jpg
Image:IOC106 (14).jpg
Image:IOC106 (15).jpg
Image:IOC106 (17).jpg
Image:IOC106 (18).jpg
Image:IOC106 (20).jpg
Image:IOC106 (22).jpg
Image:IOC106 (24).jpg
Image:IOC106 (25).jpg
Image:IOC106 (26).jpg
Image:IOC106 (6).jpg
Image:IOC106 (7).jpg
Image:IOC106 (8).jpg
Image:IOC106 (27).jpg
Image:IOC106 (28).jpg
Image:IOC106 (29).jpg
Image:IOC106 (31).jpg
Image:IOC106 (37).jpg
Image:IOC106 (38).jpg
Image:IOC106 (39).jpg
Image:IOC106 (40).jpg
Image:IOC106 (42).jpg
Image:IOC106 (43).jpg
Image:IOC106 (44).jpg
Image:IOC106 (45).jpg
Image:IOC106 (46).jpg
Image:IOC106 (48).jpg
Image:IOC106 (49).jpg
Image:IOC106 (51).jpg
Image:IOC106 (52).jpg
Image:IOC106 (53).jpg
Image:IOC106 (55).jpg
</gallery>




== IOC recommended reading ==
== Contact ==
:»  [[IOC recommended reading |More details]]
: '''» [[MitoPedia: Respiratory states]]''' [[Image:P.jpg|link=OXPHOS  capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]]  [[Image:E.jpg|link=ETS capacity|ETS]] [[Image:L.jpg|link=LEAK  respiration|LEAK]] [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]


:» [[Oroboros Contact]]




Latest revision as of 15:15, 8 June 2020

                

MiPNet20.10 IOC106 Schroecken


Publications in the MiPMap
OROBOROS-solo.png

Schroecken AT, 2015 Oct 06-11. Oroboros O2k-Workshop on HRR and O2k-Fluorometry, IOC106.

» Bioblast pdf

Oroboros (2015-10-06) Mitochondr Physiol Network

Abstract:

O2k-Workshops.png

Oroboros O2k-Workshop on HRR and O2k-Fluorometry. Schroecken, Vorarlberg, Austria; 2015 Oct 06-11.


• O2k-Network Lab: AT_Innsbruck_Oroboros


Labels:





HRR: Oxygraph-2k 

ORO, IOC, 2015, MitoFit 


Feedback

  • Thank you for the excellent seminar and your knowledgeable staff - Robert Coker, University of Alaska, Institute of Arctic Biology (US)


Team and guest tutors

Carolina Doerrier Doerrier Carolina, Mitochondrial Application Specialist and PostDoc, Oroboros Instruments

Erich Gnaiger Gnaiger Erich, CEO, Oroboros Instruments

Florian Hoppel Hoppel Florian, PhD Student, Oroboros Instruments

Laner Verena Laner Verena, Chief Operating Officer (COO), Oroboros Instruments

Meagan McManus McManus Meagan, O2k-Network Lab: US PA Philadelphia Wallace DC

Zuzana Sumbalova Sumbalova Zuzana, O2k-Network Lab: SK Bratislava Sumbalova Z


Participants

Participant Institution
Ball Darran UK Southampton Grocott MPW: University of Southampton, Academic Unit of Cancer Sciences (UK)
Bezuidenhout Nicole ZA Cape Town Ojuka EO: University of Cape Town, Institute of South Africa Newlands, ESSM UCT Dept of Human Biology Sports Science (ZA)
Coker Robert US AK Fairbanks Coker R: University of Alaska, Institute of Arctic Biology (US)
Droescher Stephanie AT Innsbruck Oroboros : Oroboros Instruments (AT)
Einer Claudia DE Munich Zischka H: Helmholtz Zentrum München, Institute of Molecular Toxicology and Pharmacology (DE)
Garnham Jack UK Leeds Peers C: LIGHT, University of Leeds, Cardiovascular & Neuronal Remodelling (UK)
Gudiksen Anders DK Copenhagen Pilegaard H: University of Copenhagen (DK)
Haas Clarissa BR Porto Alegre Souza DOG: Universidade Federal do Rio Grande do Sul, Biochemistry Department (BR)
Halling Jens F DK Copenhagen Pilegaard H: University of Copenhagen (DK)
Hansen Allan DK Copenhagen Poulsen HE: Rigshospitalet Copenhagen, Clinical Pharmacology (DK)
Jackson Christopher FI Helsinki Wartiovaara A: Biomedicum Helsinki, Research Program for Molecular Neurology (FI)
Lamberti Giorgia AT Innsbruck Huber L: Medical University of Innsbruck, Biocenter (AT)
Lindquist Carine NO Bergen Berge RK: University of Bergen, Department of Clinical Science (NO)
Matsumoto Junichi JP Sapporo Yokota T : Hokkaido University, Department of Cardiovascular Medicine, Hokkaido University Graduate School of Medicine (JP)
Molina Anthony JA US NC Winston-Salem Molina AJA: Department of Internal Medicine, Section on Gerontology and Geriatric Medicine, Wake Forest School of Medicine (US, NC)
Nakajima Takayuki JP Sapporo Yokota T : Hokkaido University, Department of Cardiovascular Medicine, Hokkaido University Graduate School of Medicine (JP)
Overby Peter DK Copenhagen Pilegaard H: University of Copenhagen (DK)
Pirinen Eija FI Helsinki Pirinen E: University of Helsinki, Research Program for Molecular Neurology (FI)
Scaini Giselli US TX Houston Reus GZ: Departmentof Psychiatry and Behavioral Sciences, Center for Experimental Models in Psychiatry, UT Health Science Center at Houston (TX, US)
Schmitt Sabine DE Munich Zischka H: Helmholtz Zentrum München, Institute of Molecular Toxicology and Pharmacology (DE)
Sharaf Mahmoud CA Charlottetown Kamunde C: University of Prince Edward Island, Department of Biomedical Sciences (CA)
Smith James ZA Cape Town Ojuka EO: University of Cape Town, Institute of South Africa Newlands, ESSM UCT Dept of Human Biology Sports Science (ZA)
Takada Shingo JP Sapporo Yokota T : Hokkaido University, Department of Cardiovascular Medicine, Hokkaido University Graduate School of Medicine (JP)
Tyrrell Daniel US NC Winston-Salem Molina AJA: Department of Internal Medicine, Section on Gerontology and Geriatric Medicine, Wake Forest School of Medicine (US, NC)
Volani Chiara AT Innsbruck Weiss G: Medical University of Innsbruck, Department of Inner Medicine (AT)
Ward Marie Louise NZ Auckland Ward ML: The University of Auckland, Department of Physiology, Faculty of Medical and Health Sciences (NZ)
Weiss Alexander AT Innsbruck Jansen-Duerr P: Medical University of Innsbruck, Research Institute for Biomedical Aging Research (AT)
West Malcolm UK Southampton Grocott MPW: University of Southampton, Academic Unit of Cancer Sciences (UK)


IOC106 results

O2k-Demo experiment on fish heart homogenate (Shredder)

O2k-Demo experiment on intact cells

Demo analysis of hands-on experiments on permeabilized cells


Impressions from the IOC106

  • Hands-on experiment: Robert Coker, Carina Lindquist and Verena Laner.

  • Eija Pirinen.

  • In Action: Meagan McManus.

  • Anthony J. Molina, Giorgia Lamberti and Peter Overby.

  • Clarissa Haas working experimentally.

  • Marie-Luise Ward, Darran Ball and Malcolm West.

  • Demonstration of the POS-Service by Carolina Doerrier with Chiara Volani, Allan Hanson and Giorgia Lamberti.

  • Are there any bubbles? Mahmoud Sharaf, Junichi Matsumot and Giselli Scaini.

  • Claudia Einer, Alexander Weiss and Sabine Schmitt doing the POS-Service.

  • Working in groups - running different experiments on 7 O2k.

  • Discussions with Erich about protocols.

  • Waiting for stabilization of the flux.

  • Discussing some of the results with Erich.

  • Florian Hoppel ‘helping-out’

  • Data analysis by … and Chiara Volani

  • Giselli Scaini presenting her work.

  • Mahmoud Sharaf giving an overview of his research.

  • Sunny weather in Schroecken – use it for a group picture.

  • On the walk to the Alpmuseum …

  • Having fun and trying to jump as high as possible for the picture.

  • Group picture with Edwin (Alpmuseum’s director) in the front.

  • You can never have enough good group pictures.

  • Anthony Molina in the lead.

  • The view to the hotel on the walk to the Alpmuseum.

  • Vegetation changes already its colour. James Smith in the lead.

  • Having a break.

  • Claudia Einer, Carolina Doerrier, Sabine Schmitt and Carine Lindquist.

  • Science and hiking collaboration: Anthony Molina and Zuzana Sumbalova.

  • Enjoying the view.

  • Chiara Volani and Daniel Tyrrell in the front …

  • Not everybody is fully concentrated on the walk…

  • A well-deserved break in the ‘Auenfelder Hütte’.

  • A toast on a successful O2k-Workshop.

  • The ORO-Team: Verena, Erich, Caro (left to right).

  • Stopping for a picture is necessary in this picturesque-environment.

  • Zuzana Sumbalova and Clarissa Haas.

  • Erich and Edwin

  • Live demonstration how to make wool by Anna.

  • Guide Edwin is telling stories of the past.

  • Robert Coker and Anna.


O2k perspectives - abstracts

Abstracts for the O2k perspectives evenings provide participants an opportunity to present and discuss their work, including projects in progress, methodological details and conceptual perspectives.

» Abstract Guidelines - Abstract Deadline: 2015-Sep-24


 Has title
Lindquist 2015 Abstract IOC106Alteraas EK, Lindquist C, Berge RK, Bjorndal B (2015) OXPHOS capacity in mouse liver altered by modified fatty acids that inhibit or stimulate beta-oxidation. Mitochondr Physiol Network 20.10.
Scaini 2015 Abstract IOC106Scaini G, Gomes LM, Carvalho-Silva M, Arent CO, Mariot E, Quevedo J, Streck EL (2015) Co-administration of omega-3 fatty acids and mood stabilizers reverses the impairment of bioenergetic parameters induced by fenproporex administration in hippocampus of rats. Mitochondr Physiol Network 20.10.
Ward 2015 Abstract IOC106Power A, Crossman D, Hickey AJ, Ward M (2015) Investigating mitochondrial function in a rat model of right ventricular hypertrophy. Mitochondr Physiol Network 20.10.
Volani 2015 Abstract IOC106Volani C, Haschka D, Demetz E, Doerrier C, Gnaiger E, Weiss G (2015) Determination of mitochondrial respiration in peripheral blood mononuclear cells. Mitochondr Physiol Network 20.10.
Sharaf 2015 Abstract IOC106Sharaf MS, Van den Heuvel MR, Stevens D, Kamunde C (2015) Mechanisms of mitochondrial import and interactions of zinc and calcium. Mitochondr Physiol Network 20.10.


IOC recommended reading

» More details
» MitoPedia: Respiratory states OXPHOS ROUTINE ETS LEAK ROX


General information

This O2k-Workshop provides a detailed and practical introduction to the O2k and the O2k-Fluorometer, with real-time analysis by DatLab and applications of the TIP2k. Demo experiments illustrate the principle and show the unique advantages and limitations of simultaneous monitoring of oxygen concentration, respiration, hydrogen peroxide production or mt-membrane potential.

» Information on travel and venue: IOC Schroecken.


Contact

» [email protected]
» Oroboros Contact


MitoGlobal
O2k-Workshops are listed as MitoGlobal Events.
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