WG1 MitoEAGLE protocols, terminology, documentation

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Working Groups

Short-Term Scientific Missions

Management Committee

Members

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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping

WG1 MitoEAGLE protocols, terminology, documentation

WG1 Action

Standard operating procedures and user requirement document: Protocols, terminology, documentation

» WG1 Project application

Many analyses lack statistical power within a single study. Statistical combination of different studies is often difficult due to lack of methodological standardization and harmonization.

WG1 Action

WG Leader

Marko Vendelin, Dr. - [email protected]

WG Co-Leader

Dominik Pesta, Mag. Dr. - [[email protected] [email protected]]

Participants and discussion

» Talk:WG1 MitoEAGLE protocols, terminology, documentation

Task groups

TG leaders: suggestions discussed at the MitoFit Science Camp 2016 Kuehtai AT, and updated according to the MitoEAGLE_MC_Meeting_2016_Brussels_BE.

TG participants: please send us an Email to join: [email protected]

SUIT protocols and terminology

Task group 1.1

Coupling states and coupling control ratios: consensus statements

TG leaders: Gnaiger Erich AT / Hoppel Charles L US

» Gnaiger E, Aasander Frostner E, Abdul Karim N, Abumrad NA, Acuna-Castroviejo D, Adiele RC et al (2019) Mitochondrial respiratory states and rates. MitoFit Preprint Arch doi:10.26124/mitofit:190001. - »Bioblast link«

Task group 1.2

SUIT protocols: nomenclature consistent with concept of database

TG leader: Doerrier Velasco Carolina A AT, Gnaiger Erich AT

TG participants: Attimonelli Marcella IT, Krako Nina RS, Vendelin Marko EE

Task group 1.3

Experimental details in SUIT

TG leaders: Chicco Adam J US/ Hickey Anthony J NZ/ Doerrier Velasco Carolina A AT

TG participants: Casado Pinna Marta ES, Endlicher Rene CZ, Krako Nina RS, Kucera Otto CZ, Sobotka Ondrej CZ, Stankova Pavla CZ

» MitoPedia: Sample preparations

Action

» MitoPedia: SUIT

» MitoPedia: Media for respirometry

» MitoPedia: Substrates and metabolites

» MiPNet21.06 SUIT RP

References

1. Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. - »Bioblast link«
2. Gnaiger E (2017) Quality first: a paradigm shift in mt-respiratory control towards global connection. Abstract MitoEAGLE Barcelona 2017. - »Bioblast link«
3. Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840, DOI:10.1038/s41598-017-02789-8. - »Bioblast link«
4. Ost M, Doerrier C, Gama-Perez P, Moreno-Gomez S (2018) Analysis of mitochondrial respiratory function in tissue biopsies and blood cells. Curr Opin Clin Nutr Metab Care 21:336-42. - »Bioblast link«

Instrumental platforms: comparisons

Task group 1.4

Instrumental platforms: comparisons

TG leaders: Molina Anthony JA US

TG participants: Canto Carles CH (suggested), Genova Maria Luisa IT, Tronstad Karl Johan NO (suggested), Vendelin Marko EE, Zorzano Antonio ES (suggested)

Knowledge management system and proficiency test

Task group 1.5

Knowledge management system

TG participants: Vendelin Marko EE, Garcia-Roves Pablo ES

• Towards and beyond a mitochondrial physiology database: filter and amplify more signals from this data, making them more meaningful and valuable for research and medicine.

Task group 1.6

Proficiency test

TG leaders: Gnaiger Erich AT/ Doerrier Velasco Carolina A AT

TG participants: Genova Maria Luisa IT

Action

» MitoFit-KMP

» MitoFit Quality Control System

» MiPMap

» MitoFit proficiency test

» MiPNet21.07 MitoFit DatLab PT

References

1. Database of Mammalian Metabolic Enzymes ::::» MetabolicEnzymeDatabase

Documentation

Task group 1.7

Documentation

TG leaders: Arnould Thierry BE / Votion Dominique-Marie BE

TG participants: Gnaiger Erich AT

Action

» Preprints for Gentle Science

» What we can learn from strategies on clinical trials

Preprints for MitoEAGLE

1. Gnaiger E, Aasander Frostner E, Abdul Karim N, Abumrad NA, Acuna-Castroviejo D, Adiele RC et al (2019) Mitochondrial respiratory states and rates. MitoFit Preprint Arch doi:10.26124/mitofit:190001. - »Bioblast link«
2. Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of oxidative phosphorylation by temperature in the heart. bioRxiv doi10.1101/103457. - »Bioblast link«
3. Lemieux H, Subarsky P, Doblander C, Wurm M, Troppmair J, Gnaiger E (2017) Impairment of mitochondrial respiratory function as an early biomarker of apoptosis induced by growth factor removal. bioRxiv doi: https://doi.org/10.1101/151480. - »Bioblast link«

Cores of reproducibility, data availability

» Wagner P (2017) Cores of reproducibility in physiology (CORP): Advancing the corpus of physiological knowledge. J Appl Physiol 122:89-90.

» Curran-Everett D (2017) CORP: Minimizing the chances of false positives and false negatives. J Appl Physiol 122:91-5.

• Think less about a simple P value and more about the scientific importance of the confidence interval bounds for some experimental result. Even a convincing P value of 0.005 can be associated with a confidence interval whose bounds indicate an effect that is scientifically inconsequential.
• Rely on repeated studies to accumulate evidence for some biological phenomenon.

» Data availability statements in PLOS journals

Dissemination and training

Task group 1.8

Translation to community in general

TG leader: Makrecka-Kuka Marina LV (Dissemination Coordinator), Rattan Suresh DK

TG participants: Krako Nina RS, Irving Brian A US

Task group 1.9

Dissemination Scientific community

TG leaders: Makrecka-Kuka Marina LV / Gnaiger Erich AT

TG participants: Ali Sameh S EG, Cervinkova Zuzana CZ, Chicco Adam J US, Coen Paul M US, Eide Lars NO, Garcia-Roves Pablo M ES, Gasparre Giuseppe IT, Genova Maria Luisa IT, Haendeler Judith DE, Iyer Shilpa US, Krako Nina RS, Laner Verena AT, Mayan Maria D ES, Mishmar Dan IL, Nehlin Jan DK, Palmeira Carlos PT, Paterlini Serena IT, Porcelli Anna Maria IT, Rao Raj US, Schaefer Patrick DE, Tronstad Karl Johan NO, Trougakos Ioannis GR, Volani Chiara AT, Votion Dominique-Marie BE

The Project Repository Journal - https://www.europeandissemination.eu/the-project-repository-journal

In February 2019 the first edition of The Project Repository Journal will be launched. Fully open access and dedicated to EU funded projects it will become the first point of call for project dissemination. Containing information on future funding calls and open access guidelines it will feature projects from across all different funding mechanisms and allow them to publish results, annual reports or general updates about the progress of the project.

Task group 1.10

Training, Short Term Scientific Missions

TG leader: Labieniec-Watala Magdalena PL, Klaus Susanne DE

TG participants: Ali Sameh S EG, Cervinkova Zuzana CZ, Chicco Adam J US, Coen Paul M US, Eide Lars NO, Garcia-Roves Pablo M ES, Gasparre Giuseppe IT, Genova Maria Luisa IT, Haendeler Judith DE, Iyer Shilpa US, Krako Nina RS, Mayan Maria D ES, Mishmar Dan IL, Nehlin Jan DK, Palmeira Carlos PT, Paterlini Serena IT, Porcelli Anna Maria IT, Rao Raj US, Schaefer Patrick DE, Tronstad Karl Johan NO, Trougakos Ioannis GR, Volani Chiara AT, Votion Dominique-Marie BE

Action

» STSM cooperation NeuroVive and Oroboros Instruments Eleonor Åsander Frostner – guest tutor at international workshop

» MitoEAGLE on Facebook

» Media:Dissemination plan MITOEAGLE.docx

» Short-Term Scientific Missions MitoEAGLE

Progress

» Database

» WG1 summary - Belgrade

» MitoEAGLE WG1 Coimbra 2019 - WG1 and WG3 protocol development and liver

» COST Connect workshop on Data Sharing Brussel 2019 - “COST Connect” workshop on the topic of Data Sharing

» WG1 Q Meeting

» WG1 summary - Jurmala

WG1: MitoEAGLE protocols, terminology, documentation

• Final discussion of [[Gnaiger 2019 MitoFit Preprint Arch |MitoFit Preprint Arch doi:10.26124/mitofit:190001. - »Bioblast link«]] - All participants are invited to submit comments and improvements, and those who are not yet included as co-authors are invited to join.
• Mitochondrial respiratory control by fuel substrates and specific inhibitors of respiratory enzymes: Building blocks of mitochondrial physiology Part 2
• Training and knowledge management concept - MiPschool Tromso-Bergen 2018
• Towards a MitoEAGLE proficiency training workshop
• Report on ECI, ITC and gender balance in COST Action MitoEAGLE
• Gnaiger E, Aasander Frostner E, Abdul Karim N, Abumrad NA, Acuna-Castroviejo D, Adiele RC et al (2019) Mitochondrial respiratory states and rates. MitoFit Preprint Arch doi:10.26124/mitofit:190001. - »Bioblast link«

» WG1 summary - Hradec Kralove

» WG1 summary - Obergurgl

» Minutes with WG1 summary - Barcelona

» Minutes with WG1 summary - Verona

Open call

» Network discussion forum WG1: cytochrome c effect

References

» ISO/IEC 17043:2010 General requirements for proficiency testing

» ISO 13528:2015 Statistical methods for use in proficiency testing by interlaboratory comparison

» ISO 15189:2012 Medical laboratories — Particular requirements for quality and competence

» ISO 17511:2003 In vitro diagnostic medical devices

» ISO 9001:2015 Quality management systems - requirements

» ISO 10012:2003 Measurement management systems

General requirements for the competence of testing and calibration laboratories » ISO/IEC 17025:2005